[Hemoglobin C Variant Affecting Glycated Hemoglobin Test Results: A Rare Case Report].
Capillary electrophoresis
Glycated hemoglobin
Hemoglobin variant
High-performance liquid Chromatography
Journal
Sichuan da xue xue bao. Yi xue ban = Journal of Sichuan University. Medical science edition
ISSN: 1672-173X
Titre abrégé: Sichuan Da Xue Xue Bao Yi Xue Ban
Pays: China
ID NLM: 101162609
Informations de publication
Date de publication:
May 2023
May 2023
Historique:
medline:
31
5
2023
pubmed:
30
5
2023
entrez:
29
5
2023
Statut:
ppublish
Résumé
Hemoglobin (Hb) variants are common factors that affect the results of glycosylated hemoglobin (A1C) tests. Hemoglobin variants react differently to different testing methods. Herein, we presented the first ever report of the effect of hemoglobin C (Hb C) on the test results of A1C in the Chinese population. High performance liquid chromatography (HPLC) and capillary electrophoresis were performed to measure A1C. Hemoglobin electrophoresis was conducted to identify the hemoglobin variants. Hb sequencing was performed to determine the mutation sites on the β chain. HPLC showed decreased A1C results, which could be corrected by electrophoresis, but the electrophoresis graph still showed abnormal peaks. The hemoglobin electrophoresis results suggested that there were hemoglobin variants, which hemoglobin sequencing results revealed to be Hb C. Uncommon variations in a specific population tend to be overlooked. To avoid clinical decision-making being affected by the results of a single test, we recommend that an explanatory reporting model be routinely adopted for A1C tests so that all reports always contain explanatory notes for the testing methodology and analysis of the graphs.
Identifiants
pubmed: 37248601
doi: 10.12182/20230560209
pmc: PMC10475414
doi:
Substances chimiques
Hemoglobin C
9008-00-8
Glycated Hemoglobin
0
Hemoglobins, Abnormal
0
Types de publication
Case Reports
English Abstract
Journal Article
Langues
chi
Sous-ensembles de citation
IM
Pagination
659-662Informations de copyright
Copyright© by Editorial Board of Journal of Sichuan University (Medical Sciences).
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