Stable isotope labeling as a promising tool for rapid drug susceptibility testing in Neisseria gonorrhoeae.
Ciprofloxacin
MALDI-TOF
Resistance detection
SILAC
Sexually transmitted infection
Journal
Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology]
ISSN: 1678-4405
Titre abrégé: Braz J Microbiol
Pays: Brazil
ID NLM: 101095924
Informations de publication
Date de publication:
Sep 2023
Sep 2023
Historique:
received:
02
11
2022
accepted:
07
02
2023
pmc-release:
01
06
2024
medline:
11
9
2023
pubmed:
1
6
2023
entrez:
31
5
2023
Statut:
ppublish
Résumé
The world is heading towards an era of intractable and impending untreatable N. gonorrhoeae, thereby underlining the significance of rapid and accurate prediction of drug resistance as an indispensable need of the hour. In the present study, we optimized and evaluated a stable isotope labeling-based approach using the MALDI-TOF MS (Matrix-Assisted Laser Desorption/Ionization-Time of Flight Mass Spectrometry) for rapid and reliable detection of ciprofloxacin and azithromycin resistance in N. gonorrhoeae. All the isolates were cultured under three varied condition setups viz. medium supplemented with normal lysine, heavy lysine (isotope), and heavy lysine along with the antibiotics (ciprofloxacin/azithromycin), respectively. After incubation, spectra were acquired using the MALDI-TOF MS which were further screened for unique patterns (media-specific spectra) to differentiate drug-susceptible and resistant isolates. The results of the stable isotope labeling assay were comparable to the results of phenotypic methods used for susceptibility testing.
Identifiants
pubmed: 37258877
doi: 10.1007/s42770-023-00996-2
pii: 10.1007/s42770-023-00996-2
pmc: PMC10485193
doi:
Substances chimiques
Azithromycin
83905-01-5
Lysine
K3Z4F929H6
Anti-Bacterial Agents
0
Ciprofloxacin
5E8K9I0O4U
Culture Media, Conditioned
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
1819-1825Informations de copyright
© 2023. The Author(s) under exclusive licence to Sociedade Brasileira de Microbiologia.
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