A Programmable CRISPR/Cas9 Toolkit Improves Lycopene Production in Bacillus subtilis.
Bacillus subtilis
CRISPR
MEP pathway
genome editing
lycopene
microbial fermentation
Journal
Applied and environmental microbiology
ISSN: 1098-5336
Titre abrégé: Appl Environ Microbiol
Pays: United States
ID NLM: 7605801
Informations de publication
Date de publication:
28 06 2023
28 06 2023
Historique:
medline:
30
6
2023
pubmed:
5
6
2023
entrez:
5
6
2023
Statut:
ppublish
Résumé
Bacillus subtilis has been widely used and generally recognized as a safe host for the production of recombinant proteins, high-value chemicals, and pharmaceuticals. Thus, its metabolic engineering attracts significant attention. Nevertheless, the limited availability of selective markers makes this process difficult and time-consuming, especially in the case of multistep biosynthetic pathways. Here, we employ CRISPR/Cas9 technology to build an easy cloning toolkit that addresses commonly encountered obstacles in the metabolic engineering of B. subtilis, including the chromosomal integration locus, promoter, terminator, and guide RNA (gRNA) target. Six promoters were characterized, and the promoter strengths ranged from 0.9- to 23-fold that of the commonly used strong promoter P
Identifiants
pubmed: 37272803
doi: 10.1128/aem.00230-23
pmc: PMC10305015
doi:
Substances chimiques
Lycopene
SB0N2N0WV6
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e0023023Déclaration de conflit d'intérêts
The authors declare no conflict of interest.
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