RNA polymerase II depletion from the inactive X chromosome territory is not mediated by physical compartmentalization.
Journal
Nature structural & molecular biology
ISSN: 1545-9985
Titre abrégé: Nat Struct Mol Biol
Pays: United States
ID NLM: 101186374
Informations de publication
Date de publication:
08 2023
08 2023
Historique:
received:
15
04
2021
accepted:
28
04
2023
medline:
23
8
2023
pubmed:
9
6
2023
entrez:
8
6
2023
Statut:
ppublish
Résumé
Subnuclear compartmentalization has been proposed to play an important role in gene regulation by segregating active and inactive parts of the genome in distinct physical and biochemical environments. During X chromosome inactivation (XCI), the noncoding Xist RNA coats the X chromosome, triggers gene silencing and forms a dense body of heterochromatin from which the transcription machinery appears to be excluded. Phase separation has been proposed to be involved in XCI, and might explain the exclusion of the transcription machinery by preventing its diffusion into the Xist-coated territory. Here, using quantitative fluorescence microscopy and single-particle tracking, we show that RNA polymerase II (RNAPII) freely accesses the Xist territory during the initiation of XCI. Instead, the apparent depletion of RNAPII is due to the loss of its chromatin stably bound fraction. These findings indicate that initial exclusion of RNAPII from the inactive X reflects the absence of actively transcribing RNAPII, rather than a consequence of putative physical compartmentalization of the inactive X heterochromatin domain.
Identifiants
pubmed: 37291424
doi: 10.1038/s41594-023-01008-5
pii: 10.1038/s41594-023-01008-5
pmc: PMC10442225
doi:
Substances chimiques
RNA Polymerase II
EC 2.7.7.-
Heterochromatin
0
Chromatin
0
RNA, Untranslated
0
RNA, Long Noncoding
0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
1216-1223Subventions
Organisme : NCI NIH HHS
ID : U54 CA231641
Pays : United States
Informations de copyright
© 2023. The Author(s).
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