Diversity of B Cell Populations and Ig Repertoire in Human Lungs.
Journal
Journal of immunology (Baltimore, Md. : 1950)
ISSN: 1550-6606
Titre abrégé: J Immunol
Pays: United States
ID NLM: 2985117R
Informations de publication
Date de publication:
01 08 2023
01 08 2023
Historique:
received:
12
05
2022
accepted:
25
05
2023
medline:
19
7
2023
pubmed:
14
6
2023
entrez:
14
6
2023
Statut:
ppublish
Résumé
The human lung carries a unique microbiome adapted to the air-filled, mucous-lined environment, the presence of which requires an immune system capable of recognizing harmful populations while preventing reactions toward commensals. B cells in the lung play a key role in pulmonary immunity, generating Ag-specific Abs, as well as cytokine secretion for immune activation and regulation. In this study, we compared B cell subsets in human lungs versus circulating cells by analyzing patient-paired lung and blood samples. We found a significantly smaller pool of CD19+, CD20+ B cells in the lung relative to the blood. CD27+, IgD-, class-switched memory B cells (Bmems) composed a larger proportion of the pool of pulmonary B cells. The residency marker CD69 was also significantly higher in the lung. We also sequenced the Ig V region genes (IgVRGs) of class-switched Bmems that do, or do not, express CD69. We observed the IgVRGs of pulmonary Bmems to be as heavily mutated from the unmutated common ancestor as those in circulation. Furthermore, we found progenies within a quasi-clone can gain or lose CD69 expression, regardless of whether the parent clone expressed the residency marker. Overall, our results show that despite its vascularized nature, human lungs carry a unique proportion of B cell subsets. The IgVRGs of pulmonary Bmems are as diverse as those in blood, and progenies of Bmems retain the ability to gain or lose residency.
Identifiants
pubmed: 37314411
pii: 265706
doi: 10.4049/jimmunol.2200340
pmc: PMC10352589
mid: NIHMS1904911
doi:
Substances chimiques
Antigens, CD19
0
Tumor Necrosis Factor Receptor Superfamily, Member 7
0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Langues
eng
Sous-ensembles de citation
IM
Pagination
486-496Subventions
Organisme : NHLBI NIH HHS
ID : R33 HL137081
Pays : United States
Informations de copyright
Copyright © 2023 by The American Association of Immunologists, Inc.
Références
J Immunol Methods. 1987 Dec 4;105(1):9-14
pubmed: 3680965
Nat Immunol. 2019 Jan;20(1):97-108
pubmed: 30510223
Nat Rev Immunol. 2021 Jun;21(6):347-362
pubmed: 33442032
J Immunol. 2009 Aug 1;183(3):1983-9
pubmed: 19620319
Cell Rep. 2021 Jun 22;35(12):109286
pubmed: 34161770
J Immunol. 2016 Mar 15;196(6):2514-2525
pubmed: 26851219
Viral Immunol. 2020 May;33(4):282-293
pubmed: 32023188
Nature. 2004 Jan 22;427(6972):355-60
pubmed: 14737169
Annu Rev Physiol. 2016;78:481-504
pubmed: 26527186
J Exp Med. 2014 Jun 2;211(6):1243-56
pubmed: 24821911
Cell Res. 2014 Feb;24(2):135-6
pubmed: 24343577
J Exp Med. 2019 Jul 1;216(7):1487-1496
pubmed: 31160320
F1000Res. 2013 Apr 03;2:103
pubmed: 24555054
Sci Immunol. 2022 Jan 28;7(67):eabf5314
pubmed: 35089815
J Clin Invest. 2021 Jun 1;131(11):
pubmed: 34060477
Clin Microbiol Rev. 2006 Apr;19(2):315-37
pubmed: 16614252
Adv Immunol. 2010;107:187-241
pubmed: 21034975
Blood. 2020 Dec 10;136(24):2774-2785
pubmed: 32750113
Mucosal Immunol. 2012 Jul;5(4):444-54
pubmed: 22472773
Cell. 2015 Jul 2;162(1):184-97
pubmed: 26095251
Immunity. 2014 Dec 18;41(6):886-97
pubmed: 25526304
Nature. 2014 Mar 20;507(7492):366-370
pubmed: 24572363
Mucosal Immunol. 2017 Mar;10(2):299-306
pubmed: 27966551
J Immunol. 1994 Feb 1;152(3):1228-36
pubmed: 8301128
J Allergy Clin Immunol. 2013 Apr;131(4):933-57; quiz 958
pubmed: 23540615
J Cell Mol Med. 2021 Mar;25(5):2621-2632
pubmed: 33481318
Blood. 2000 Apr 1;95(7):2312-20
pubmed: 10733501
Comp Biochem Physiol A Mol Integr Physiol. 2001 May;129(1):151-61
pubmed: 11369540
Nat Med. 2004 Sep;10(9):927-34
pubmed: 15311275