Vacuum differential mobility spectrometry combined with column-switching liquid chromatography- mass spectrometry for the analysis of pyrrolizidine alkaloids in tea samples.
Column switching LC-MS
Differential mobility spectrometry
Pyrrolizidine alkaloids
Supercritical fluid Chromatography-MS
Tea
Journal
Journal of chromatography. A
ISSN: 1873-3778
Titre abrégé: J Chromatogr A
Pays: Netherlands
ID NLM: 9318488
Informations de publication
Date de publication:
30 Aug 2023
30 Aug 2023
Historique:
received:
08
03
2023
revised:
15
06
2023
accepted:
18
06
2023
medline:
9
8
2023
pubmed:
23
6
2023
entrez:
22
6
2023
Statut:
ppublish
Résumé
The benefit of combining liquid chromatography (LC), supercritical fluid chromatography (SFC) and vacuum Differential Mobility Spectrometry - Mass Spectrometry (vDMS-MS) was investigated for the analysis of fourteen diastereomeric pyrrolizidine alkaloids (PA); intermedine, echinatine, lycopsamine, indicine, intermedine-N-oxide, echinatine-N-oxide, indicine-N-oxide, lycopsamine-N-oxide, senecivernine, senecionine, jacobine, senecivernine-N-oxide, senecionine-N-oxide, retrorsine. The mobile phase composition (15-100% MeOH and ACN), flow rate (8-100 µL/min), vDMS cell pressure, and F value showed an effect on the mobility behavior of the analytes. At 15% MeOH with a flow rate of 100 µL/min and 33 mbar vDMS pressure, 8 out 14 PA could be partially or totally separated by vDMS-MS. As well as providing an additional separation dimension vDMS improved the selectivity and a 5-minute assay method was developed for the quantification of 10 out of 14 single diastereomeric PA in tea samples, using a short LC column-switching and hyphenated to vDMS-MS in the selected ion monitoring mode. The performance of the method was found to be comparable with a 12-minute standard LC-MS/MS method using detection in the selected reaction monitoring mode. Additionally, the combination of vDMS and SFC-MS was investigated and suggests that the mixture of CO
Identifiants
pubmed: 37348223
pii: S0021-9673(23)00400-4
doi: 10.1016/j.chroma.2023.464174
pii:
doi:
Substances chimiques
Pyrrolizidine Alkaloids
0
Oxides
0
Tea
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
464174Informations de copyright
Copyright © 2023 The Author(s). Published by Elsevier B.V. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of Competing Interest The authors declare the following financial interests/personal relationships which may be considered as potential competing interests: Hopfgartner, G reports equipment, drugs, or supplies was provided by Shimadzu Research Laboratory Europe Ltd. Patrick Knight reports a relationship with Shimadzu Research Laboratory Europe Ltd that includes: employment. Patrick Knight is employee of Shimadzu Research Laboratory (Europe) Ltd. which developed and supplied the vDMS