PagMYB151 facilitates proline accumulation to enhance salt tolerance of poplar.
Genetic transformation
PagMYB151
Poplar
Proline
Salt stress
Journal
BMC genomics
ISSN: 1471-2164
Titre abrégé: BMC Genomics
Pays: England
ID NLM: 100965258
Informations de publication
Date de publication:
22 Jun 2023
22 Jun 2023
Historique:
received:
23
03
2023
accepted:
16
06
2023
medline:
26
6
2023
pubmed:
23
6
2023
entrez:
22
6
2023
Statut:
epublish
Résumé
Poplar is one of the main urban and rural greening and shade tree species in the northern hemisphere, but its growth and development is always restricted by salt stress. R2R3-MYB transcription factor family is commonly involved in many biological processes during plant growth and stress endurance. In this study, PagMYB151 (Potri.014G035100) one of R2R3-MYB members related to salt stress and expressed in both nucleus and cell membrane was cloned from Populus alba × P. glandulosa to perfect the salt tolerance mechanism. Morphological and physiological indexes regulated by PagMYB151 were detected using the PagMYB151 overexpression (OX) and RNA interference (RNAi) transgenic poplar lines. Under salt stress conditions, compared with RNAi and the non-transgenic wild-type (WT) plants, the plant height, both aboveground and underground part fresh weight of OX was significantly increased. In addition, OX has a longer and finer root structure and a larger root surface area. The root activity of OX was also enhanced, which was significantly different from RNAi but not from WT under salt treatment. Under normal conditions, the stomatal aperture of OX was larger than WT, whereas this phenotype was not obvious after salt stress treatment. In terms of physiological indices, OX enhanced the accumulation of proline but reduced the toxicity of malondialdehyde to plants under salt stress. Combing with the transcriptome sequencing data, 6 transcription factors induced by salt stress and co-expressed with PagMYB151 were identified that may cooperate with PagMYB151 to function in salt stress responding process. This study provides a basis for further exploring the molecular mechanism of poplar PagMYB151 transcription factor under abiotic stress.
Identifiants
pubmed: 37349699
doi: 10.1186/s12864-023-09459-2
pii: 10.1186/s12864-023-09459-2
pmc: PMC10286439
doi:
Substances chimiques
Plant Proteins
0
Proline
9DLQ4CIU6V
Transcription Factors
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
345Subventions
Organisme : Innovation and Entrepreneurship Key Incubation Project of College Students in 2022
ID : No.15, No30
Organisme : Natural Science Foundation of Shanxi Province
ID : 20210302123425, 202103021223150
Organisme : Biobreeding Project of Shanxi Agricultural University
ID : YZGC140
Organisme : Scientific and Technological Innovation Programs of Higher Education Institutions in Shanxi
ID : 2021L099
Organisme : Opening Project of State Key Laboratory of Tree Genetics and Breeding
ID : K2021104
Informations de copyright
© 2023. The Author(s).
Références
Plant Physiol Biochem. 2019 Feb;135:385-394
pubmed: 30616113
Front Plant Sci. 2020 Oct 16;11:571881
pubmed: 33178243
Trends Plant Sci. 2010 Oct;15(10):573-81
pubmed: 20674465
New Phytol. 2018 Jan;217(2):523-539
pubmed: 29205383
Int J Mol Sci. 2023 Jan 02;24(1):
pubmed: 36614232
Plant Cell Environ. 2014 Sep;37(9):2064-76
pubmed: 24506578
Plants (Basel). 2013 Dec 05;2(4):769-85
pubmed: 27137403
Plant Physiol. 2009 Feb;149(2):981-93
pubmed: 19091872
Front Plant Sci. 2020 Jan 15;10:1722
pubmed: 32010174
Tree Physiol. 2022 Aug 6;42(8):1628-1645
pubmed: 35225347
Plant Signal Behav. 2011 Oct;6(10):1499-502
pubmed: 21904117
Plant Cell Rep. 2014 Oct;33(10):1687-96
pubmed: 24969399
Plant Cell. 2014 Jul;26(7):2920-38
pubmed: 25035403
Amino Acids. 2008 Nov;35(4):753-9
pubmed: 18379856
Tree Physiol. 2016 Jul;36(7):896-908
pubmed: 26941290
Plants (Basel). 2020 Apr 10;9(4):
pubmed: 32290272
Plant Physiol. 2002 Oct;130(2):639-48
pubmed: 12376631
Plant Sci. 2019 Dec;289:110259
pubmed: 31623781
BMC Plant Biol. 2022 Apr 9;22(1):186
pubmed: 35395726
Plant Mol Biol. 2021 May;106(1-2):157-172
pubmed: 33704646
Plant Cell. 2019 Mar;31(3):663-686
pubmed: 30538157
Nat Genet. 2000 May;25(1):25-9
pubmed: 10802651
Tree Physiol. 2022 Aug 6;42(8):1678-1692
pubmed: 35220440
Front Plant Sci. 2015 Jul 20;6:544
pubmed: 26257754
Plant Biotechnol J. 2016 Mar;14(3):849-60
pubmed: 26228739
J Exp Bot. 2021 May 28;72(12):4319-4332
pubmed: 33831169
Plant Biotechnol J. 2018 Aug;16(8):1514-1528
pubmed: 29406575
Plant Physiol. 2020 Nov;184(3):1273-1290
pubmed: 32958560
Plant Physiol Biochem. 2017 May;114:100-110
pubmed: 28285084
Proteomes. 2014 Mar 04;2(1):85-106
pubmed: 28250372
Plant Mol Biol. 2016 Feb;90(3):267-79
pubmed: 26646286
Tree Physiol. 2012 Oct;32(10):1313-20
pubmed: 22971569
Plant Biol (Stuttg). 2022 Mar;24(2):344-355
pubmed: 34921493
Trends Plant Sci. 2020 Nov;25(11):1117-1130
pubmed: 32675014
Plants (Basel). 2022 Nov 09;11(22):
pubmed: 36432761
Nat Commun. 2021 Nov 18;12(1):6663
pubmed: 34795219
Plant Sci. 2020 Jan;290:110301
pubmed: 31779889
Dent Mater. 2010 Nov;26(11):1059-67
pubmed: 20688380
Tree Physiol. 2020 Jan 1;40(1):46-59
pubmed: 31728530
Trends Plant Sci. 2022 Mar;27(3):287-300
pubmed: 34580024
Hortic Res. 2020 Jul 1;7:96
pubmed: 32637124
Plant Cell Rep. 2015 May;34(5):831-41
pubmed: 25627252
Environ Sci Pollut Res Int. 2015 Mar;22(6):4056-75
pubmed: 25398215
Front Plant Sci. 2021 Jun 04;12:669143
pubmed: 34149765
Plant Physiol. 2012 Dec;160(4):1996-2006
pubmed: 23077242
Nucleic Acids Res. 2000 Jan 1;28(1):27-30
pubmed: 10592173
Mol Biol Evol. 2016 Jul;33(7):1870-4
pubmed: 27004904
Plant Cell Rep. 2018 Feb;37(2):225-237
pubmed: 29079898
Virology. 2014 Jan 20;449:207-14
pubmed: 24418554
New Phytol. 2022 Apr;234(1):179-196
pubmed: 35023174
New Phytol. 2023 Jan;237(1):232-250
pubmed: 36264565
EMBO J. 1987 Dec 1;6(12):3553-8
pubmed: 3428265
Clin Chim Acta. 1993 Jan 31;214(1):103-4
pubmed: 8453769