Extracting nanoscale membrane morphology from single-molecule localizations.
Journal
Biophysical journal
ISSN: 1542-0086
Titre abrégé: Biophys J
Pays: United States
ID NLM: 0370626
Informations de publication
Date de publication:
08 08 2023
08 08 2023
Historique:
received:
08
03
2023
revised:
17
05
2023
accepted:
15
06
2023
pmc-release:
08
08
2024
medline:
11
8
2023
pubmed:
25
6
2023
entrez:
25
6
2023
Statut:
ppublish
Résumé
Membrane surface reconstruction at the nanometer scale is required for understanding mechanisms of subcellular shape change. This historically has been the domain of electron microscopy, but extraction of surfaces from specific labels is a difficult task in this imaging modality. Existing methods for extracting surfaces from fluorescence microscopy have poor resolution or require high-quality super-resolution data that are manually cleaned and curated. Here, we present NanoWrap, a new method for extracting surfaces from generalized single-molecule localization microscopy data. This makes it possible to study the shape of specifically labeled membranous structures inside cells. We validate NanoWrap using simulations and demonstrate its reconstruction capabilities on single-molecule localization microscopy data of the endoplasmic reticulum and mitochondria. NanoWrap is implemented in the open-source Python Microscopy Environment.
Identifiants
pubmed: 37355772
pii: S0006-3495(23)00398-3
doi: 10.1016/j.bpj.2023.06.010
pmc: PMC10432223
pii:
doi:
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
3022-3030Subventions
Organisme : Wellcome Trust
ID : 203285/B/16/Z
Pays : United Kingdom
Organisme : NIGMS NIH HHS
ID : R01 GM118486
Pays : United States
Organisme : NIGMS NIH HHS
ID : T32 GM007223
Pays : United States
Organisme : NIBIB NIH HHS
ID : T32 EB019941
Pays : United States
Commentaires et corrections
Type : UpdateOf
Informations de copyright
Copyright © 2023 Biophysical Society. Published by Elsevier Inc. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of interests J.B. discloses a significant financial interest in Bruker Corp., Hamamatsu Photonics, and panluminate Inc.
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