Biocatalytic Foams from Microdroplet-Formulated Self-Assembling Enzymes.
enzymes
flow biocatalysis
microreactors
monodisperse foams
porous materials
Journal
Advanced materials (Deerfield Beach, Fla.)
ISSN: 1521-4095
Titre abrégé: Adv Mater
Pays: Germany
ID NLM: 9885358
Informations de publication
Date de publication:
Sep 2023
Sep 2023
Historique:
revised:
16
06
2023
received:
27
04
2023
medline:
28
9
2023
pubmed:
26
6
2023
entrez:
26
6
2023
Statut:
ppublish
Résumé
Industrial biocatalysis plays an important role in the development of a sustainable economy, as enzymes can be used to synthesize an enormous range of complex molecules under environmentally friendly conditions. To further develop the field, intensive research is being conducted on process technologies for continuous flow biocatalysis in order to immobilize large quantities of enzyme biocatalysts in microstructured flow reactors under conditions that are as gentle as possible in order to realize efficient material conversions. Here, monodisperse foams consisting almost entirely of enzymes covalently linked via SpyCatcher/SpyTag conjugation are reported. The biocatalytic foams are readily available from recombinant enzymes via microfluidic air-in-water droplet formation, can be directly integrated into microreactors, and can be used for biocatalytic conversions after drying. Reactors prepared by this method show surprisingly high stability and biocatalytic activity. The physicochemical characterization of the new materials is described and exemplary applications in biocatalysis are shown using two-enzyme cascades for the stereoselective synthesis of chiral alcohols and the rare sugar tagatose.
Identifiants
pubmed: 37358068
doi: 10.1002/adma.202303952
doi:
Substances chimiques
Alcohols
0
Enzymes, Immobilized
0
Enzymes
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
e2303952Subventions
Organisme : Helmholtz association : Materials Systems Engineering
Organisme : Helmholtz Enterprise project
ID : HE-2020-26
Organisme : Fonds der Chemischen Industrie
Informations de copyright
© 2023 The Authors. Advanced Materials published by Wiley-VCH GmbH.
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