Phylogenetic analyses of antimicrobial resistant Corynebacterium striatum strains isolated from a nosocomial outbreak in a tertiary hospital in China.
Humans
Phylogeny
Cross Infection
/ epidemiology
Tertiary Care Centers
Gentian Violet
RNA, Ribosomal, 16S
/ genetics
Corynebacterium Infections
/ epidemiology
Microbial Sensitivity Tests
Anti-Bacterial Agents
/ pharmacology
Anti-Infective Agents
Disease Outbreaks
Drug Resistance, Multiple, Bacterial
/ genetics
16S rRNA
Antibiotic resistance
Biofilms
Corynebacterium striatum
Phylogenetic analysis
Journal
Antonie van Leeuwenhoek
ISSN: 1572-9699
Titre abrégé: Antonie Van Leeuwenhoek
Pays: Netherlands
ID NLM: 0372625
Informations de publication
Date de publication:
Sep 2023
Sep 2023
Historique:
received:
23
04
2022
accepted:
19
06
2023
medline:
28
7
2023
pubmed:
27
6
2023
entrez:
27
6
2023
Statut:
ppublish
Résumé
Corynebacterium striatum is an emerging, multidrug-resistant pathogen that frequently causes nosocomial infections worldwide. This study aimed to investigate phylogenetic relationship and presence of genes responsible for antimicrobial resistance among C. striatum strains associated with an outbreak at the Shanxi Bethune Hospital, China, in 2021. Fecal samples were collected from 65 patients with C. striatum infection at Shanxi Bethune Hospital between February 12, 2021 and April 12, 2021. C. striatum isolates were identified by 16S rRNA and rpoB gene sequencing. E-test strips were used to examine the antimicrobial susceptibility of the isolates. Whole-genome sequencing and bioinformatics analysis were employed to assess the genomic features and identify antimicrobial resistance genes of the isolates. Crystal violet staining was conducted to determine the ability of biofilm formation of each isolate. A total of 64 C. striatum isolates were identified and categorized into 4 clades based on single nucleotide polymorphisms. All isolates were resistant to penicillin, meropenem, ceftriaxone, and ciprofloxacin but susceptible to vancomycin and linezolid. Most isolates were also resistant to tetracycline, clindamycin, and erythromycin, with susceptibility rates of 10.77, 4.62, and 7.69%, respectively. Genomic analysis revealed 14 antimicrobial resistance genes in the isolates, including tetW, ermX, and sul1. Crystal violet staining showed that all isolates formed biofilms on the abiotic surface. Four clades of multidrug-resistant C. striatum spread in our hospitals possibly due to the acquisition of antimicrobial resistance genes.
Identifiants
pubmed: 37368178
doi: 10.1007/s10482-023-01855-8
pii: 10.1007/s10482-023-01855-8
pmc: PMC10371919
doi:
Substances chimiques
Gentian Violet
J4Z741D6O5
RNA, Ribosomal, 16S
0
Anti-Bacterial Agents
0
Anti-Infective Agents
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
907-918Subventions
Organisme : Financial Aid Project of Shanxi Province
ID : 2021RC0329
Informations de copyright
© 2023. The Author(s).
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