Evolutionary origin of B family DNA-dependent DNA polymerases from retrotranscriptases.
Ancestor
DNA origin
DNA polymerases
Journal
Bio Systems
ISSN: 1872-8324
Titre abrégé: Biosystems
Pays: Ireland
ID NLM: 0430773
Informations de publication
Date de publication:
Aug 2023
Aug 2023
Historique:
received:
17
06
2023
revised:
20
06
2023
accepted:
21
06
2023
medline:
11
7
2023
pubmed:
30
6
2023
entrez:
29
6
2023
Statut:
ppublish
Résumé
The evolution of DNA and DNA polymerases was a crucial step in the evolution of life on Earth. In the present work, we reconstruct the ancestral sequence and structure for the B family polymerases. Using comparative analyses, we infer the transient state between the ancestor retrotranscriptase and the contemporary B family DNA polymerases. Exonuclease motif was detected in the primary ancestral sequence, as well as an elongation-functioning motif. It is remarkable that the ancestral molecule is more comparable to the retrotranscriptases in terms of structural domains, even though we discovered similarities in the primary sequence with proteins from the B family of DNA polymerases. The present B family proteins differ structurally from retrotranscriptases the most, although the reconstruction of the ancestor protein was able to capture the transitional steps between these two families of polymerases.
Identifiants
pubmed: 37385536
pii: S0303-2647(23)00138-7
doi: 10.1016/j.biosystems.2023.104963
pii:
doi:
Substances chimiques
DNA-Directed DNA Polymerase
EC 2.7.7.7
DNA
9007-49-2
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
104963Informations de copyright
Copyright © 2023 Elsevier B.V. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of competing interest The authors declare that there is no conflict of interest in this manuscript.