Investigation of osmotic shock effect on pulsed electric field treated S. cerevisiae yeast cells.

Saccharomyces cerevisiae Osmotic Pressure Yeast, Dried Osmolar Concentration Cell Membrane Glycerol Kinase

Journal

Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288

Informations de publication

Date de publication:
29 06 2023
Historique:
received: 16 05 2023
accepted: 26 06 2023
medline: 3 7 2023
pubmed: 30 6 2023
entrez: 29 6 2023
Statut: epublish

Résumé

Pulsed electric field (PEF) treatment is known to cause plasma membrane permeabilization of microorganisms, an effect known as electroporation. PEF treatment is very attractive since it can achieve permeabilization with or without lethal damage in accordance with desired results. This study aimed to expand the accomplishment of electroporation outcomes by applying sudden post-PEF osmotic composition change of the media. Changes in yeast cells' viability, size and plasma membrane regeneration rate were evaluated. However, we still have questions about the intracellular biochemical processes responsible for plasma membrane recovery after electroporation. Our suggested candidate is the high osmolarity glycerol (HOG) kinase pathway. The HOG pathway in Saccharomyces cerevisiae yeasts is responsible for volume recovery after dangerous shape modifications and intracellular water disbalance caused by environmental osmotic pressure changes. Thus, we evaluated the HOG pathway inactivation effect on S. cerevisiae's reaction to PEF treatment. Results showed that Hog1 deficient S. cerevisiae cells were considerably more sensitive to electric field treatment, confirming a link between the HOG pathway and S. cerevisiae recovery process after electroporation. By suddenly changing the osmolarity of the media after PEF we influenced the cells' plasma membrane recovery rate, severity of permeabilization and survivability of yeast cells. Studies of electroporation in combination with various treatments might improve electric field application range, efficiency, and optimization of the process.

Identifiants

DOI: 10.1038/s41598-023-37719-4 PMID: 37386124 PMC: PMC10310692
pubmed: 37386124
doi: 10.1038/s41598-023-37719-4
pii: 10.1038/s41598-023-37719-4
pmc: PMC10310692
doi:

Substances chimiques

Glycerol Kinase EC 2.7.1.30

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

10573

Informations de copyright

© 2023. The Author(s).

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Auteurs

Greta Gančytė (G)

Laboratory of Bioelectrics, Center for Physical Sciences and Technology, State Research Institute, Sauletekio Ave. 3, 10257, Vilnius, Lithuania. greta.gancyte@ftmc.lt.

Povilas Šimonis (P)

Laboratory of Bioelectrics, Center for Physical Sciences and Technology, State Research Institute, Sauletekio Ave. 3, 10257, Vilnius, Lithuania.

Arūnas Stirkė (A)

Laboratory of Bioelectrics, Center for Physical Sciences and Technology, State Research Institute, Sauletekio Ave. 3, 10257, Vilnius, Lithuania.
Micro and Nanodevices Laboratory, Institute of Solid State Physics, University of Latvia, Kengaraga Str. 8, Riga, 1063, Latvia.

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Classifications MeSH

questionsmedicales.fr › Eucaryotes › Champignons › Levures › Saccharomyces › Saccharomyces cerevisiae › Investigation of osmotic shock effect on...
questionsmedicales.fr › Phénomènes chimiques › Perméabilité › Pression osmotique › Investigation of osmotic shock effect on...
questionsmedicales.fr › Aliments et boissons › Aliments › Compléments alimentaires › Levure sèche › Investigation of osmotic shock effect on...
questionsmedicales.fr › Phénomènes chimiques › Concentration osmolaire › Investigation of osmotic shock effect on...
questionsmedicales.fr › Cellules › Structures cellulaires › Membrane cellulaire › Investigation of osmotic shock effect on...
questionsmedicales.fr › Enzymes et coenzymes › Enzymes › Transferases › Phosphotransferases › Phosphotransferases (Alcohol Group Acceptor) › Glycerol kinase › Investigation of osmotic shock effect on...