A liquid chromatography tandem mass spectrometry method for plasma glucagon.

Glucagon Tandem Mass Spectrometry / methods Chromatography, Liquid / methods Immunoassay Solid Phase Extraction / methods Chromatography, High Pressure Liquid / methods
Glucagon LC-MSMS Peptide

Journal

Analytical biochemistry
ISSN: 1096-0309
Titre abrégé: Anal Biochem
Pays: United States
ID NLM: 0370535

Informations de publication

Date de publication:
15 08 2023
Historique:
received: 23 02 2023
revised: 29 06 2023
accepted: 30 06 2023
medline: 12 7 2023
pubmed: 3 7 2023
entrez: 2 7 2023
Statut: ppublish

Résumé

Glucagon is a peptide involved in controlling the body's blood glucose levels. The majority of analytical methods used for its quantitation are based on immunoassays that suffer from cross-reactivity with other peptides. For accurate routine analysis a liquid chromatography tandem mass spectrometry (LC-MSMS) was developed. Glucagon was extracted from plasma samples by a combination of protein precipitation using ethanol and mixed anion exchange solid phase extraction. Linearity for glucagon was above 0.99 (r

Identifiants

DOI: 10.1016/j.ab.2023.115229 PMID: 37393974
pubmed: 37393974
pii: S0003-2697(23)00194-X
doi: 10.1016/j.ab.2023.115229
pii:
doi:

Substances chimiques

Glucagon 9007-92-5

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

115229

Informations de copyright

Crown Copyright © 2023. Published by Elsevier Inc. All rights reserved.

Déclaration de conflit d'intérêts

Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.

Auteurs

Van Long Nguyen (VL)

NSW Health Pathology, Department of Chemical Pathology, Royal Prince Alfred Hospital, Camperdown, NSW, 2050, Australia; Natural and Gentle Edge Company, Emu Plains, NSW, 2750, Australia. Electronic address: vanlong.nguyen@health.nsw.gov.au.

Phillip Tuckwell (P)

NSW Health Pathology, Department of Chemical Pathology, Royal Prince Alfred Hospital, Camperdown, NSW, 2050, Australia; Natural and Gentle Edge Company, Emu Plains, NSW, 2750, Australia.

Andrea Ireland (A)

NSW Health Pathology, Department of Chemical Pathology, Royal Prince Alfred Hospital, Camperdown, NSW, 2050, Australia.

Michael Fitzpatrick (M)

NSW Health Pathology, Department of Chemical Pathology, Royal Prince Alfred Hospital, Camperdown, NSW, 2050, Australia.

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Classifications MeSH

questionsmedicales.fr › Acides aminés, peptides et protéines › Peptides › Hormones peptidiques › Hormones pancréatiques › Glucagon › A liquid chromatography tandem mass...
questionsmedicales.fr › Techniques d'investigation › Techniques de chimie analytique › Spectrométrie de masse › Spectrométrie de masse en tandem › A liquid chromatography tandem mass...
questionsmedicales.fr › Techniques d'investigation › Techniques de chimie analytique › Chromatographie › Chromatographie en phase liquide › A liquid chromatography tandem mass...
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questionsmedicales.fr › Techniques d'investigation › Techniques de chimie analytique › Fractionnement chimique › Extraction en phase solide › A liquid chromatography tandem mass...
questionsmedicales.fr › Techniques d'investigation › Techniques de chimie analytique › Chromatographie › Chromatographie en phase liquide › Chromatographie en phase liquide à haute performance › A liquid chromatography tandem mass...