Systems Analysis of Highly Multiplexed CRISPR-Base Editing in Streptomycetes.

Gene Editing CRISPR-Cas Systems / genetics Clustered Regularly Interspaced Short Palindromic Repeats / genetics RNA, Guide, CRISPR-Cas Systems Actinomycetales / genetics Systems Analysis
CRISPR CRISPR-BEST Streptomyces base editor cytidine deaminase multiplexed genome engineering

Journal

ACS synthetic biology
ISSN: 2161-5063
Titre abrégé: ACS Synth Biol
Pays: United States
ID NLM: 101575075

Informations de publication

Date de publication:
18 08 2023
Historique:
medline: 21 8 2023
pubmed: 4 7 2023
entrez: 4 7 2023
Statut: ppublish

Résumé

CRISPR tools, especially Cas9n-sgRNA guided cytidine deaminase base editors such as CRISPR-BEST, have dramatically simplified genetic manipulation of streptomycetes. One major advantage of CRISPR base editing technology is the possibility to multiplex experiments in genomically instable species. Here, we demonstrate scaled up Csy4 based multiplexed genome editing using CRISPR-mcBEST in

Identifiants

DOI: 10.1021/acssynbio.3c00188 PMID: 37402223
pubmed: 37402223
doi: 10.1021/acssynbio.3c00188
doi:

Substances chimiques

RNA, Guide, CRISPR-Cas Systems 0

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

2353-2366

Auteurs

Christopher M Whitford (CM)

The Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark, 2800 Kgs. Lyngby, Denmark.

Tetiana Gren (T)

The Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark, 2800 Kgs. Lyngby, Denmark.

Emilia Palazzotto (E)

The Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark, 2800 Kgs. Lyngby, Denmark.

Sang Yup Lee (SY)

Metabolic and Biomolecular Engineering National Research Laboratory, Department of Chemical and Biomolecular Engineering (BK21 Plus Program), Center for Systems and Synthetic Biotechnology, Institute for the BioCentury, Korea Advanced Institute of Science and Technology (KAIST), Daejeon 305-701, Republic of Korea.
ORCID: 0000-0003-0599-3091

Yaojun Tong (Y)

State Key Laboratory of Microbial Metabolism, Joint International Research Laboratory of Metabolic and Developmental Sciences, School of Life Sciences and Technology, Shanghai Jiao Tong University, Shanghai 200240, China.
ORCID: 0000-0003-4042-2462

Tilmann Weber (T)

The Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark, 2800 Kgs. Lyngby, Denmark.
ORCID: 0000-0002-8260-5120

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Classifications MeSH

questionsmedicales.fr › Techniques d'investigation › Techniques génétiques › Génie génétique › Édition de gène › Systems Analysis of Highly Multiplexed...
questionsmedicales.fr › Phénomènes génétiques › Régulation de l'expression des gènes › Épigenèse génétique › Extinction de l'expression des gènes › Systèmes CRISPR-Cas › Systems Analysis of Highly Multiplexed...
questionsmedicales.fr › Phénomènes génétiques › Structures génétiques › Génome › Composants de génome › Séquences répétées en tandem › Clustered regularly interspaced short palindromic repeats › Systems Analysis of Highly Multiplexed...
questionsmedicales.fr › Acides nucléiques, nucléotides et nucléosides › Acides nucléiques › ARN › ARN non traduit › Petit ARN non traduit › Systèmes CRISPR-Cas, ARN, guide › Systems Analysis of Highly Multiplexed...
questionsmedicales.fr › Bactéries › Bactéries à Gram positif › Actinomycetales › Systems Analysis of Highly Multiplexed...
questionsmedicales.fr › Sciences de l'information › Analyse des systèmes › Systems Analysis of Highly Multiplexed...