Beet mosaic virus expression of a betalain transcription factor allows visual virus tracking in Beta vulgaris.
BvMYB1
Caryophyllales
beet mosaic virus
betalain biosynthesis
infectious cDNA clone
virus tracking
Journal
Molecular plant pathology
ISSN: 1364-3703
Titre abrégé: Mol Plant Pathol
Pays: England
ID NLM: 100954969
Informations de publication
Date de publication:
10 2023
10 2023
Historique:
revised:
19
06
2023
received:
13
03
2023
accepted:
19
06
2023
medline:
18
9
2023
pubmed:
6
7
2023
entrez:
6
7
2023
Statut:
ppublish
Résumé
In the field of plant virology, the usage of reverse genetic systems has been reported for multiple purposes. One is understanding virus-host interaction by labelling viral cDNA clones with fluorescent protein genes to allow visual virus tracking throughout a plant, albeit this visualization depends on technical devices. Here we report the first construction of an infectious cDNA full-length clone of beet mosaic virus (BtMV) that can be efficiently used for Agrobacterium-mediated leaf inoculation with high infection rate in Beta vulgaris, being indistinguishable from the natural virus isolate regarding symptom development and vector transmission. Furthermore, the BtMV clone was tagged with the genes for the monomeric red fluorescent protein or the Beta vulgaris BvMYB1 transcription factor, which activates the betalain biosynthesis pathway. The heterologous expression of BvMYB1 results in activation of betalain biosynthesis genes in planta, allowing visualization of the systemic BtMV spread with the naked eye as red pigmentation emerging throughout beet leaves. In the case of BtMV, the BvMYB1 marker system is stable over multiple mechanical host passages, allows qualitative as well as quantitative virus detection and offers an excellent opportunity to label viruses in plants of the order Caryophyllales, allowing an in-depth investigation of virus-host interactions on the whole plant level.
Identifiants
pubmed: 37410356
doi: 10.1111/mpp.13372
pmc: PMC10502864
doi:
Substances chimiques
Transcription Factors
0
Betalains
37279-84-8
DNA, Complementary
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
1319-1329Informations de copyright
© 2023 The Authors. Molecular Plant Pathology published by British Society for Plant Pathology and John Wiley & Sons Ltd.
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