Collision-Induced Dissociation of Citrullinated Peptide Anions.
CID
MS/MS
citrullination
citrulline
deamidation
deprotonated peptides
negative mode
Journal
Journal of the American Society for Mass Spectrometry
ISSN: 1879-1123
Titre abrégé: J Am Soc Mass Spectrom
Pays: United States
ID NLM: 9010412
Informations de publication
Date de publication:
02 Aug 2023
02 Aug 2023
Historique:
pmc-release:
06
07
2024
medline:
3
8
2023
pubmed:
7
7
2023
entrez:
6
7
2023
Statut:
ppublish
Résumé
Peptide identification by positive electrospray ionization (ES+) tandem mass spectrometry (MS/MS) is a well-established strategy in proteomics. Several research groups reported the usefulness of negative electrospray ionization (ES-) for gaining complementary structural information on peptides and their post-translational modifications (PTM) compared to ES+. Fragmentation of citrullinated peptides has not been previously explored in ES-. In this study, 9 peptides containing citrulline residues were investigated in ES- by stepwise collision energy-dependent measurements on a QTOF instrument and a Q-Orbitrap instrument. Our results of high resolution and mass accuracy show the favored citrulline-selective loss of HNCO from these peptide precursors and their fragments─similarly to that in ES+─along with y-NH
Identifiants
pubmed: 37414397
doi: 10.1021/jasms.3c00044
pmc: PMC10402709
doi:
Substances chimiques
Citrulline
29VT07BGDA
Peptides
0
Anions
0
Ions
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
1569-1575Références
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