Selective Proteolysis of Activated Transcriptional Factor by NIR-Responsive Palindromic DNA Thalidomide Conjugate Inhibits the Canonical Smad Pathway.

Transcription Factors / metabolism Thalidomide / pharmacology Proteolysis Gene Expression Regulation DNA / metabolism Transforming Growth Factor beta / metabolism
TGF-β/Smad aptamer optical control protein degradation transcription factor upconversion nanoparticles (UCNPs)

Journal

Small (Weinheim an der Bergstrasse, Germany)
ISSN: 1613-6829
Titre abrégé: Small
Pays: Germany
ID NLM: 101235338

Informations de publication

Date de publication:
Nov 2023
Historique:
revised: 26 06 2023
received: 24 03 2023
medline: 2 11 2023
pubmed: 7 7 2023
entrez: 7 7 2023
Statut: ppublish

Résumé

Dysfunctional transcription factors that activate abnormal expressions of specific proteins are often associated with the progression of various diseases. Despite being attractive drug targets, the lack of druggable sites has dramatically hindered their drug development. The emergence of proteolysis targeting chimeras (PROTACs) has revitalized the drug development of many conventional hard-to-drug protein targets. Here, the use of a palindromic double-strand DNA thalidomide conjugate (PASTE) to selectively bind and induce proteolysis of targeted activated transcription factor (PROTAF) is reported. The selective proteolysis of the dimerized phosphorylated receptor-regulated Smad2/3 and inhibition of the canonical Smad pathway validates PASTE-mediated PROTAF. Further aptamer-guided active delivery of PASTE and near-infrared light-triggered PROTAF are demonstrated. Great potential in using PASTE for the selective degradation of the activated transcription factor is seen, providing a powerful tool for studying signaling pathways and developing precision medicines.

Identifiants

DOI: 10.1002/smll.202302525 PMID: 37415558
pubmed: 37415558
doi: 10.1002/smll.202302525
doi:

Substances chimiques

Transcription Factors 0
Thalidomide 4Z8R6ORS6L
DNA 9007-49-2
Transforming Growth Factor beta 0

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

e2302525

Subventions

Organisme : NSFC
ID : 22204046
Organisme : Excellent Young Scholar Program of Natural Science Foundation of Hunan Province
ID : 2023JJ20002
Organisme : National Key Research Program
ID : 2019YFA0905800
Organisme : Scientific Research Fund of Hunan Provincial Education Department
ID : 21B0815
Organisme : Scientific Research Fund of Hunan Provincial Education Department
ID : 22B0874

Informations de copyright

© 2023 Wiley-VCH GmbH.

Références

A. C. Lai, C. M. Crews, Nat. Rev. Drug Discovery 2017, 16, 101.
D. A. Nalawansha, C. M. Crews, Cell Chem. Biol. 2020, 27, 998.
J. J. Zhuang, Q. Liu, D. L. Wu, L. Tie, Acta Pharmacol. Sin. 2022, 43, 2474.
a) T. I. Lee, R. A. Young, Cell 2013, 152, 1237;
b) S. A. Lambert, A. Jolma, L. F. Campitelli, P. K. Das, Y. Yin, M. Albu, X. Chen, J. Taipale, T. R. Hughes, M. T. Weirauch, Cell 2018, 172, 650.
Y. Li, J. Song, P. Zhou, J. Zhou, S. Xie, J. Med. Chem. 2022, 65, 10183.
M. Bekes, D. R. Langley, C. M. Crews, Nat. Rev. Drug Discovery 2022, 21, 181.
Y. Yu, X. H. Feng, Curr. Opin. Cell Biol. 2019, 61, 56.
B. Schmierer, C. S. Hill, Nat. Rev. Mol. Cell Biol. 2007, 8, 970.
a) H. Yadav, C. Quijano, A. K. Kamaraju, O. Gavrilova, R. Malek, W. Chen, P. Zerfas, D. Zhigang, E. C. Wright, C. Stuelten, P. Sun, S. Lonning, M. Skarulis, A. E. Sumner, T. Finkel, S. G. Rane, Cell Metab. 2011, 14, 67;
b) X. M. Meng, P. M. Tang, J. Li, H. Y. Lan, Front. Physiol. 2015, 6, 82.
R. J. Akhurst, A. Hata, Nat. Rev. Drug Discovery 2012, 11, 790.
J. H. Kang, M. Y. Jung, X. Yin, M. Andrianifahanana, D. M. Hernandez, E. B. Leof, J. Clin. Invest. 2017, 127, 2541.
A. Hata, Y. G. Chen, Cold Spring Harbor Perspect. Biol. 2016, 8, a022061.
a) J. Shao, Y. Yan, D. Ding, D. Wang, Y. He, Y. Pan, W. Yan, A. Kharbanda, H. Y. Li, H. Huang, Adv. Sci. (Weinheim, Ger.) 2021, 8, 2102555;
b) A. Ghidini, A. Clery, F. Halloy, F. H. T. Allain, J. Hall, Angew. Chem., Int. Ed. Engl. 2021, 60, 3163.
J. Liu, H. Chen, H. U. Kaniskan, L. Xie, X. Chen, J. Jin, W. Wei, J. Am. Chem. Soc. 2021, 143, 8902.
A. Jolma, J. Yan, T. Whitington, J. Toivonen, K. R. Nitta, P. Rastas, E. Morgunova, M. Enge, M. Taipale, G. Wei, K. Palin, J. M. Vaquerizas, R. Vincentelli, N. M. Luscombe, T. R. Hughes, P. Lemaire, E. Ukkonen, T. Kivioja, J. Taipale, Cell 2013, 152, 327.
J. Yamamoto, T. Ito, Y. Yamaguchi, H. Handa, Chem. Soc. Rev. 2022, 51, 6234.
E. Aragon, Q. Wang, Y. Zou, S. M. Morgani, L. Ruiz, Z. Kaczmarska, J. Su, C. Torner, L. Tian, J. Hu, W. Shu, S. Agrawal, T. Gomes, J. A. Marquez, A. K. Hadjantonakis, M. J. Macias, J. Massague, Genes Dev. 2019, 33, 1506.
E. R. Neptune, P. A. Frischmeyer, D. E. Arking, L. Myers, T. E. Bunton, B. Gayraud, F. Ramirez, L. Y. Sakai, H. C. Dietz, Nat. Genet. 2003, 33, 407.
a) X. Zhang, Y. Li, Y. Zhang, J. Song, Q. Wang, L. Zheng, D. Liu, PLoS One 2013, 8, 58719;
b) J. Zhou, Y. Dabiri, R. A. Gama-Brambila, S. Ghafoory, M. Altinbay, A. Mehrabi, M. Golriz, B. Blagojevic, S. Reuter, K. Han, A. Seidel, I. Dikic, S. Wolfl, X. Cheng, J. Cell Biol. 2022, 221, 202012097.
Z. X. Chong, S. K. Yeap, W. Y. Ho, PeerJ 2021, 9, 11165.
a) Y. Wu, B. Lin, Y. Lu, L. Li, K. Deng, S. Zhang, H. Zhang, C. Yang, Z. Zhu, Angew. Chem., Int. Ed. Engl. 2023, 62, 202218106;
b) S. Shigdar, B. Schrand, P. H. Giangrande, V. de Franciscis, Mol. Ther. 2021, 29, 2396.
a) M. Xiao, W. Lai, T. Man, B. Chang, L. Li, A. R. Chandrasekaran, H. Pei, Chem. Rev. 2019, 119, 11631;
b) L. Li, H. Xing, J. Zhang, Y. Lu, Acc. Chem. Res. 2019, 52, 2415.
a) S. He, F. Gao, J. Ma, H. Ma, G. Dong, C. Sheng, Angew. Chem., Int. Ed. Engl. 2021, 60, 23299;
b) R. Jia, Y. Wang, W. Ma, J. Huang, H. Sun, B. Chen, H. Cheng, X. He, K. Wang, Anal. Chem. 2022, 94, 5937.
P. A. Humphreys, S. Woods, C. A. Smith, N. Bates, S. A. Cain, R. Lucas, S. J. Kimber, ACS Synth. Biol. 2020, 9, 3067.
a) K. T. G. Samarasinghe, S. Jaime-Figueroa, M. Burgess, D. A. Nalawansha, K. Dai, Z. Hu, A. Bebenek, S. A. Holley, C. M. Crews, Cell Chem. Biol. 2021, 28, 648;
b) X. Zhang, Z. Zhang, X. Xue, T. Fan, C. Tan, F. Liu, Y. Tan, Y. Jiang, ACS Pharmacol. Transl. Sci. 2022, 5, 1109.
J. Zhao, Z. Di, L. Li, Angew. Chem., Int. Ed. Engl. 2022, 61, 202204277.
J. Zhang, P. Zhao, W. Li, L. Ye, L. Li, Z. Li, M. Li, Angew. Chem., Int. Ed. Engl. 2022, 61, 202117562.

Auteurs

Min Hou (M)

State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Biomedical Sciences, College of Chemistry and Chemical Engineering, Hunan University, Changsha, 410082, China.
School of Physics and Chemistry, Hunan First Normal University, Changsha, 410205, China.

Rui Guo (R)

State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Biomedical Sciences, College of Chemistry and Chemical Engineering, Hunan University, Changsha, 410082, China.

Tianyu Ren (T)

State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Biomedical Sciences, College of Chemistry and Chemical Engineering, Hunan University, Changsha, 410082, China.

Tao Wang (T)

State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Biomedical Sciences, College of Chemistry and Chemical Engineering, Hunan University, Changsha, 410082, China.

Jian-Hui Jiang (JH)

State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Biomedical Sciences, College of Chemistry and Chemical Engineering, Hunan University, Changsha, 410082, China.
ORCID: 0000-0003-1594-4023

Jianjun He (J)

State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Biomedical Sciences, College of Chemistry and Chemical Engineering, Hunan University, Changsha, 410082, China.
ORCID: 0000-0001-9779-2662

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Classifications MeSH

questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Facteurs de transcription › Selective Proteolysis of Activated...
questionsmedicales.fr › Composés hétérocycliques › Composés hétérocycliques à cycles fusionnés › Composés hétérobicycliques › Isoindoles › Phtalimides › Thalidomide › Selective Proteolysis of Activated...
questionsmedicales.fr › Métabolisme › Protéolyse › Selective Proteolysis of Activated...
questionsmedicales.fr › Phénomènes génétiques › Régulation de l'expression des gènes › Selective Proteolysis of Activated...
questionsmedicales.fr › Acides nucléiques, nucléotides et nucléosides › Acides nucléiques › ADN › Selective Proteolysis of Activated...
questionsmedicales.fr › Facteurs biologiques › Protéines et peptides de signalisation intercellulaire › Protéines de la superfamille du TGF bêta › Facteur de croissance transformant bêta › Selective Proteolysis of Activated...