Cyclin E/CDK2 and feedback from soluble histone protein regulate the S phase burst of histone biosynthesis.
3′hExo
CDK2
CP: Cell biology
CP: Molecular biology
FLASH
Lsm11
NASP
NPAT
SLBP
histone locus body
replication-dependent histone
restriction point
Journal
Cell reports
ISSN: 2211-1247
Titre abrégé: Cell Rep
Pays: United States
ID NLM: 101573691
Informations de publication
Date de publication:
25 07 2023
25 07 2023
Historique:
received:
20
11
2022
revised:
17
04
2023
accepted:
23
06
2023
medline:
31
7
2023
pubmed:
10
7
2023
entrez:
10
7
2023
Statut:
ppublish
Résumé
Faithful DNA replication requires that cells fine-tune their histone pool in coordination with cell-cycle progression. Replication-dependent histone biosynthesis is initiated at a low level upon cell-cycle commitment, followed by a burst at the G1/S transition, but it remains unclear how exactly the cell regulates this burst in histone biosynthesis as DNA replication begins. Here, we use single-cell time-lapse imaging to elucidate the mechanisms by which cells modulate histone production during different phases of the cell cycle. We find that CDK2-mediated phosphorylation of NPAT at the restriction point triggers histone transcription, which results in a burst of histone mRNA precisely at the G1/S phase boundary. Excess soluble histone protein further modulates histone abundance by promoting the degradation of histone mRNA for the duration of S phase. Thus, cells regulate their histone production in strict coordination with cell-cycle progression by two distinct mechanisms acting in concert.
Identifiants
pubmed: 37428633
pii: S2211-1247(23)00779-9
doi: 10.1016/j.celrep.2023.112768
pmc: PMC10440735
mid: NIHMS1920500
pii:
doi:
Substances chimiques
Histones
0
Cyclin E
0
Nuclear Proteins
0
Cell Cycle Proteins
0
Cyclin-Dependent Kinase 2
EC 2.7.11.22
RNA, Messenger
0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
112768Subventions
Organisme : NCI NIH HHS
ID : DP2 CA238330
Pays : United States
Organisme : NCRR NIH HHS
ID : S10 RR026680
Pays : United States
Organisme : NIGMS NIH HHS
ID : T32 GM065103
Pays : United States
Commentaires et corrections
Type : UpdateOf
Informations de copyright
Copyright © 2023 The Author(s). Published by Elsevier Inc. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of interests The authors declare no competing interests.
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