Type IV pili facilitated natural competence in Fusobacterium nucleatum.
Bacterial genetics
DNA methyltransferase
Fusobacterium
Fusobacterium nucleatum
Natural competence
Transformation
Type IV pili
Journal
Anaerobe
ISSN: 1095-8274
Titre abrégé: Anaerobe
Pays: England
ID NLM: 9505216
Informations de publication
Date de publication:
Aug 2023
Aug 2023
Historique:
received:
10
06
2023
revised:
06
07
2023
accepted:
11
07
2023
medline:
4
10
2023
pubmed:
15
7
2023
entrez:
14
7
2023
Statut:
ppublish
Résumé
Many bacterial species naturally take up DNA from their surroundings and recombine it into their chromosome through homologous gene transfer (HGT) to aid in survival and gain advantageous functions. Herein we present the first characterization of Type IV pili facilitated natural competence in Fusobacterium nucleatum, which is a Gram-negative, anaerobic bacterium that participates in a range of infections and diseases including periodontitis, preterm birth, and cancer. Here we used bioinformatics on multiple Fusobacterium species, as well as molecular genetics to characterize natural competence in strain F. nucleatum subsp. nucleatum ATCC 23726. We bioinformatically identified components of the Type IV conjugal pilus machinery and show this is a conserved system within the Fusobacterium genus. We next validate Type IV pili in natural competence in F. nucleatum ATCC 23726 and show that gene deletions in key components of pilus deployment (pilQ) and cytoplasmic DNA import (comEC) abolish DNA uptake and chromosomal incorporation. We next show that natural competence may require native F. nucleatum DNA methylation to bypass restriction modification systems and allow subsequent genomic homologous recombination. In summary, this proof of principle study provides the first characterization of natural competence in Fusobacterium nucleatum and highlights the potential to exploit this DNA import mechanism as a genetic tool to characterize virulence mechanisms of an opportunistic oral pathogen.
Identifiants
pubmed: 37451427
pii: S1075-9964(23)00069-0
doi: 10.1016/j.anaerobe.2023.102760
pii:
doi:
Substances chimiques
RNA, Ribosomal, 16S
0
DNA, Bacterial
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
102760Informations de copyright
Copyright © 2023 Elsevier Ltd. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. Given his role as Associate Editor, Daniel Slade had no involvement in the peer review of this article and has no access to information regarding its peer review. Given his role as Editorial Board member, Blake Sanders had no involvement in the peer review of this article.