An efficient protocol for preparing linear β-manno-oligosaccharides.

Hydrolysis Oligosaccharides / chemistry beta-Mannosidase Mannans / chemistry
Cassia gum Enzymatic hydrolysis Manno-oligosaccharides Purification

Journal

Carbohydrate research
ISSN: 1873-426X
Titre abrégé: Carbohydr Res
Pays: Netherlands
ID NLM: 0043535

Informations de publication

Date de publication:
Oct 2023
Historique:
received: 27 04 2023
revised: 03 07 2023
accepted: 05 07 2023
medline: 28 8 2023
pubmed: 19 7 2023
entrez: 18 7 2023
Statut: ppublish

Résumé

Linear β-manno-oligosaccharides (l-β-MOS) are widely used to investigate oligo- and poly-saccharide structures and mannanolytic enzyme activities. l-β-MOS are also being used as prebiotic agents with potential bio-active properties. In this study, we developed an efficient protocol to prepare a series of l-β-MOS by hydrolyzing cassia gum (CG) using mannanolytic enzymes (endo-1,4-β-mannanase, α-galactosidases and β-glucosidases). By using medium pressure liquid chromatography (MPLC), we purified l-β-MOS with different degrees of polymerization (DPs). HPAEC-PAD, MALDI-TOF-MS and NMR studies confirmed that these l-β-MOS species ranged from 1,4-β-d-mannobiose to 1,4-β-d-mannononaose (DP 2-9) with >95% purity. Our results provide a robust approach to preparing l-β-MOS, thus enabling l-β-MOS to be further used in the fields of chemistry, life science, and nutritional food.

Identifiants

DOI: 10.1016/j.carres.2023.108895 PMID: 37463551
pubmed: 37463551
pii: S0008-6215(23)00157-X
doi: 10.1016/j.carres.2023.108895
pii:
doi:

Substances chimiques

Oligosaccharides 0
beta-Mannosidase EC 3.2.1.25
Mannans 0

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

108895

Informations de copyright

Copyright © 2023 Elsevier Ltd. All rights reserved.

Déclaration de conflit d'intérêts

Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.

Auteurs

Liangnan Cui (L)

Engineering Research Center of Glycoconjugates, Ministry of Education, Jilin Provincial Key Laboratory on Chemistry and Biology of Changbai Mountain Natural Drugs, School of Life Sciences, Northeast Normal University, Changchun, 130024, China. Electronic address: cuiln802@nenu.edu.cn.

Xiang Wang (X)

Engineering Research Center of Glycoconjugates, Ministry of Education, Jilin Provincial Key Laboratory on Chemistry and Biology of Changbai Mountain Natural Drugs, School of Life Sciences, Northeast Normal University, Changchun, 130024, China. Electronic address: wangx004@nenu.edu.cn.

Chao Wang (C)

Engineering Research Center of Glycoconjugates, Ministry of Education, Jilin Provincial Key Laboratory on Chemistry and Biology of Changbai Mountain Natural Drugs, School of Life Sciences, Northeast Normal University, Changchun, 130024, China. Electronic address: wangc351@nenu.com.

Yue Yan (Y)

Engineering Research Center of Glycoconjugates, Ministry of Education, Jilin Provincial Key Laboratory on Chemistry and Biology of Changbai Mountain Natural Drugs, School of Life Sciences, Northeast Normal University, Changchun, 130024, China. Electronic address: yany273@nenu.edu.cn.

Mengshan Zhang (M)

Engineering Research Center of Glycoconjugates, Ministry of Education, Jilin Provincial Key Laboratory on Chemistry and Biology of Changbai Mountain Natural Drugs, School of Life Sciences, Northeast Normal University, Changchun, 130024, China. Electronic address: zhangms253@nenu.edu.cn.

Kevin H Mayo (KH)

Department of Biochemistry, Molecular Biology and Biophysics, University of Minnesota, 6-155 Jackson Hall, Minneapolis, MN, 55455, USA. Electronic address: mayox001@umn.edu.

Lin Sun (L)

Engineering Research Center of Glycoconjugates, Ministry of Education, Jilin Provincial Key Laboratory on Chemistry and Biology of Changbai Mountain Natural Drugs, School of Life Sciences, Northeast Normal University, Changchun, 130024, China. Electronic address: sunl925@nenu.edu.cn.

Yifa Zhou (Y)

Engineering Research Center of Glycoconjugates, Ministry of Education, Jilin Provincial Key Laboratory on Chemistry and Biology of Changbai Mountain Natural Drugs, School of Life Sciences, Northeast Normal University, Changchun, 130024, China. Electronic address: zhouyf383@nenu.edu.cn.

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Classifications MeSH

questionsmedicales.fr › Phénomènes chimiques › Hydrolyse › An efficient protocol for preparing linear...
questionsmedicales.fr › Glucides › Polyosides › Oligosaccharides › An efficient protocol for preparing linear...
questionsmedicales.fr › Enzymes et coenzymes › Enzymes › Hydrolases › Glycosidases › Mannosidases › beta-Mannosidase › An efficient protocol for preparing linear...
questionsmedicales.fr › Glucides › Polyosides › Mannanes › An efficient protocol for preparing linear...