Determination of Ligand-Binding Affinity (
Journal
Journal of medicinal chemistry
ISSN: 1520-4804
Titre abrégé: J Med Chem
Pays: United States
ID NLM: 9716531
Informations de publication
Date de publication:
10 08 2023
10 08 2023
Historique:
medline:
11
8
2023
pubmed:
19
7
2023
entrez:
19
7
2023
Statut:
ppublish
Résumé
High hit rates from initial ligand-observed NMR screening can make it challenging to prioritize which hits to follow up, especially in cases where there are no available crystal structures of these hits bound to the target proteins or other strategies to provide affinity ranking. Here, we report a reproducible, accurate, and versatile quantitative ligand-observed NMR assay, which can determine
Identifiants
pubmed: 37467168
doi: 10.1021/acs.jmedchem.3c00758
pmc: PMC10424183
doi:
Substances chimiques
Ligands
0
Small Molecule Libraries
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
10617-10627Subventions
Organisme : Cancer Research UK
ID : C309/A11566
Pays : United Kingdom
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