PIF3 is phosphorylated by MAPK to modulate plant immunity.
Arabidopsis Proteins
/ genetics
Pathogen-Associated Molecular Pattern Molecules
/ metabolism
Mitogen-Activated Protein Kinases
/ metabolism
Arabidopsis
/ metabolism
Plant Immunity
/ genetics
Pseudomonas syringae
/ physiology
Plant Diseases
Gene Expression Regulation, Plant
Basic Helix-Loop-Helix Transcription Factors
/ metabolism
MAPK cascades
gene regulation
phytochrome-interacting factors
plant immunity
signal transduction
Journal
The New phytologist
ISSN: 1469-8137
Titre abrégé: New Phytol
Pays: England
ID NLM: 9882884
Informations de publication
Date de publication:
10 2023
10 2023
Historique:
received:
15
12
2022
accepted:
27
06
2023
medline:
8
9
2023
pubmed:
21
7
2023
entrez:
21
7
2023
Statut:
ppublish
Résumé
Surface-localized pattern recognition receptors perceive pathogen-associated molecular patterns (PAMPs) to activate pattern-triggered immunity (PTI). Activation of mitogen-activated protein kinases (MAPKs) represents a major PTI response. Here, we report that Arabidopsis thaliana PIF3 negatively regulates plant defense gene expression and resistance to Pseudomonas syringae DC3000. PAMPs trigger phosphorylation of PIF3. Further study reveals that PIF3 interacts with and is phosphorylated by MPK3/6. By mass spectrometry and site-directed mutagenesis, we identified the corresponding phosphorylation sites which fit for SP motif. We further show that a phospho-mimicking PIF3 variant (PIF3
Substances chimiques
Arabidopsis Proteins
0
Pathogen-Associated Molecular Pattern Molecules
0
Mitogen-Activated Protein Kinases
EC 2.7.11.24
PIF3 protein, Arabidopsis
0
Basic Helix-Loop-Helix Transcription Factors
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
372-381Informations de copyright
© 2023 The Authors New Phytologist © 2023 New Phytologist Foundation.
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