Plasma Neurofilament Light Chain Is Elevated in Adaptor Protein Complex 4-Related Hereditary Spastic Paraplegia.

SPG47 SPG50 SPG51 SPG52 adaptor protein complex-4 biomarker hereditary spastic paraplegia neurofilament light phenotypic clustering plasma

Journal

Movement disorders : official journal of the Movement Disorder Society
ISSN: 1531-8257
Titre abrégé: Mov Disord
Pays: United States
ID NLM: 8610688

Informations de publication

Date de publication:
09 2023
Historique:
revised: 15 05 2023
received: 12 02 2023
accepted: 09 06 2023
pmc-release: 01 09 2024
medline: 19 9 2023
pubmed: 24 7 2023
entrez: 24 7 2023
Statut: ppublish

Résumé

Adaptor protein complex 4-associated hereditary spastic paraplegia (AP-4-HSP) is caused by pathogenic biallelic variants in AP4B1, AP4M1, AP4E1, and AP4S1. The aim was to explore blood markers of neuroaxonal damage in AP-4-HSP. Plasma neurofilament light chain (pNfL) and glial fibrillary acidic protein (GFAP) levels were measured in samples from patients and age- and sex-matched controls (NfL: n = 46 vs. n = 46; GFAP: n = 14 vs. n = 21) using single-molecule array assays. Patients' phenotypes were systematically assessed using the AP-4-HSP natural history study questionnaires, the Spastic Paraplegia Rating Scale, and the SPATAX disability score. pNfL levels increased in AP-4-HSP patients, allowing differentiation from controls (Mann-Whitney U test: P = 3.0e-10; area under the curve = 0.87 with a 95% confidence interval of 0.80-0.94). Phenotypic cluster analyses revealed a subgroup of individuals with severe generalized-onset seizures and developmental stagnation, who showed differentially higher pNfL levels (Mann-Whitney U test between two identified clusters: P = 2.5e-6). Plasma GFAP levels were unchanged in patients with AP-4-HSP. pNfL is a potential disease marker in AP-4-HSP and can help differentiate between phenotypic subgroups. © 2023 International Parkinson and Movement Disorder Society.

Sections du résumé

BACKGROUND
Adaptor protein complex 4-associated hereditary spastic paraplegia (AP-4-HSP) is caused by pathogenic biallelic variants in AP4B1, AP4M1, AP4E1, and AP4S1.
OBJECTIVE
The aim was to explore blood markers of neuroaxonal damage in AP-4-HSP.
METHODS
Plasma neurofilament light chain (pNfL) and glial fibrillary acidic protein (GFAP) levels were measured in samples from patients and age- and sex-matched controls (NfL: n = 46 vs. n = 46; GFAP: n = 14 vs. n = 21) using single-molecule array assays. Patients' phenotypes were systematically assessed using the AP-4-HSP natural history study questionnaires, the Spastic Paraplegia Rating Scale, and the SPATAX disability score.
RESULTS
pNfL levels increased in AP-4-HSP patients, allowing differentiation from controls (Mann-Whitney U test: P = 3.0e-10; area under the curve = 0.87 with a 95% confidence interval of 0.80-0.94). Phenotypic cluster analyses revealed a subgroup of individuals with severe generalized-onset seizures and developmental stagnation, who showed differentially higher pNfL levels (Mann-Whitney U test between two identified clusters: P = 2.5e-6). Plasma GFAP levels were unchanged in patients with AP-4-HSP.
CONCLUSIONS
pNfL is a potential disease marker in AP-4-HSP and can help differentiate between phenotypic subgroups. © 2023 International Parkinson and Movement Disorder Society.

Identifiants

pubmed: 37482941
doi: 10.1002/mds.29524
pmc: PMC10529494
mid: NIHMS1914767
doi:

Substances chimiques

Adaptor Protein Complex 4 0

Types de publication

Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

1742-1750

Subventions

Organisme : NINDS NIH HHS
ID : K08 NS123552
Pays : United States
Organisme : NICHD NIH HHS
ID : P50 HD105351
Pays : United States

Informations de copyright

© 2023 International Parkinson and Movement Disorder Society.

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Auteurs

Julian E Alecu (JE)

Department of Neurology and F.M. Kirby Neurobiology Center, Boston Children's Hospital, Harvard Medical School, Boston, Massachusetts, USA.
Friedrich-Alexander-University Erlangen-Nuremberg, Erlangen, Germany.

Afshin Saffari (A)

Department of Neurology and F.M. Kirby Neurobiology Center, Boston Children's Hospital, Harvard Medical School, Boston, Massachusetts, USA.

Marvin Ziegler (M)

Department of Neurology and F.M. Kirby Neurobiology Center, Boston Children's Hospital, Harvard Medical School, Boston, Massachusetts, USA.

Catherine Jordan (C)

Department of Neurology and F.M. Kirby Neurobiology Center, Boston Children's Hospital, Harvard Medical School, Boston, Massachusetts, USA.

Amy Tam (A)

Department of Neurology and F.M. Kirby Neurobiology Center, Boston Children's Hospital, Harvard Medical School, Boston, Massachusetts, USA.

Soyoung Kim (S)

Sozialpaediatrisches Zentrum Frankfurt Mitte, Frankfurt am Main, Germany.

Edward Leung (E)

Department of Pediatrics and Child Health, University of Manitoba, Winnipeg, Manitoba, Canada.

Krzysztof Szczaluba (K)

Department of Medical Genetics, Medical University of Warsaw, Warsw, Poland.

Hanna Mierzewska (H)

Department of Neurology, Institute of Mother and Child, Warsaw, Poland.

Staci D King (SD)

Department of Neurology, Texas Children's Hospital, Houston, Texas, USA.

Filippo M Santorelli (FM)

Molecular Medicine, Neurogenetics, IRCCS Fondazione Stella Maris, Pisa, Italy.

Grace Yoon (G)

Divisions of Clinical and Metabolic Genetics and Neurology, Department of Pediatrics, The Hospital for Sick Children, University of Toronto, Toronto, Ontario, Canada.

Bianca Trombetta (B)

Alzheimer's Clinical and Translational Research Unit, Department of Neurology, Massachusetts General Hospital, Boston, Massachusetts, USA.

Pia Kivisäkk (P)

Alzheimer's Clinical and Translational Research Unit, Department of Neurology, Massachusetts General Hospital, Boston, Massachusetts, USA.

Bo Zhang (B)

Department of Neurology and F.M. Kirby Neurobiology Center, Boston Children's Hospital, Harvard Medical School, Boston, Massachusetts, USA.
ICCTR Biostatistics and Research Design Center, Boston Children's Hospital, Harvard Medical School, Boston, Massachusetts, USA.

Mustafa Sahin (M)

Department of Neurology and F.M. Kirby Neurobiology Center, Boston Children's Hospital, Harvard Medical School, Boston, Massachusetts, USA.
Rosamund Stone Zander Translational Neuroscience Center, Boston Children's Hospital, Boston, Massachusetts, USA.
Intellectual and Developmental Disabilities Research Center, Boston Children's Hospital, Boston, Massachusetts, USA.

Darius Ebrahimi-Fakhari (D)

Department of Neurology and F.M. Kirby Neurobiology Center, Boston Children's Hospital, Harvard Medical School, Boston, Massachusetts, USA.
Rosamund Stone Zander Translational Neuroscience Center, Boston Children's Hospital, Boston, Massachusetts, USA.
Intellectual and Developmental Disabilities Research Center, Boston Children's Hospital, Boston, Massachusetts, USA.
Movement Disorders Program, Department of Neurology, Boston Children's Hospital, Harvard Medical School, Boston, Massachusetts, USA.

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