Spectroscopically Orthogonal Spin Labels in Structural Biology at Physiological Temperatures.
Journal
The journal of physical chemistry. B
ISSN: 1520-5207
Titre abrégé: J Phys Chem B
Pays: United States
ID NLM: 101157530
Informations de publication
Date de publication:
03 08 2023
03 08 2023
Historique:
medline:
4
8
2023
pubmed:
25
7
2023
entrez:
25
7
2023
Statut:
ppublish
Résumé
Electron paramagnetic resonance spectroscopy (EPR) is mostly used in structural biology in conjunction with pulsed dipolar spectroscopy (PDS) methods to monitor interspin distances in biomacromolecules at cryogenic temperatures both in vitro and in cells. In this context, spectroscopically orthogonal spin labels were shown to increase the information content that can be gained per sample. Here, we exploit the characteristic properties of gadolinium and nitroxide spin labels at physiological temperatures to study side chain dynamics via continuous wave (cw) EPR at X band, surface water dynamics via Overhauser dynamic nuclear polarization at X band and short-range distances via cw EPR at high fields. The presented approaches further increase the accessible information content on biomolecules tagged with orthogonal labels providing insights into molecular interactions and dynamic equilibria that are only revealed under physiological conditions.
Identifiants
pubmed: 37490415
doi: 10.1021/acs.jpcb.3c04497
pmc: PMC10405217
doi:
Substances chimiques
Spin Labels
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.
Langues
eng
Sous-ensembles de citation
IM
Pagination
6668-6674Références
J Magn Reson. 2012 Sep;222:34-43
pubmed: 22820007
Prog Nucl Magn Reson Spectrosc. 2013 Oct;74:33-56
pubmed: 24083461
J Biol Chem. 2010 Feb 26;285(9):6636-47
pubmed: 20008353
Structure. 2021 Feb 4;29(2):114-124.e3
pubmed: 32966763
Curr Opin Struct Biol. 2022 Aug;75:102398
pubmed: 35667279
Phys Chem Chem Phys. 2012 Apr 7;14(13):4355-8
pubmed: 22362220
Cell Death Differ. 2021 Jun;28(6):1910-1925
pubmed: 33462413
Phys Chem Chem Phys. 2018 Apr 18;20(15):10470-10492
pubmed: 29617015
Nat Commun. 2022 Jul 29;13(1):4396
pubmed: 35906222
Biophys J. 2021 Nov 2;120(21):4842-4858
pubmed: 34536387
Mol Cell. 2014 Nov 20;56(4):496-505
pubmed: 25458844
Phys Chem Chem Phys. 2017 Feb 15;19(7):5127-5136
pubmed: 28139788
Phys Chem Chem Phys. 2020 Jun 24;22(24):13358-13362
pubmed: 32478770
Chemistry. 2021 Mar 17;27(16):5292-5297
pubmed: 33404074
J Phys Chem B. 2021 May 27;125(20):5358-5364
pubmed: 33998795
Contrast Media Mol Imaging. 2013 May-Jun;8(3):252-64
pubmed: 23606429
Methods Enzymol. 2022;666:79-119
pubmed: 35465930
Curr Opin Struct Biol. 2013 Oct;23(5):725-33
pubmed: 23850140
Angew Chem Int Ed Engl. 2023 Mar 20;62(13):e202212832
pubmed: 36638360
Nature. 2016 Feb 4;530(7588):45-50
pubmed: 26808899
Cell Death Differ. 2018 Jan;25(1):65-80
pubmed: 29149100
Sci Adv. 2022 Oct 14;8(41):eabn6845
pubmed: 36223470
J Phys Chem Lett. 2017 Nov 2;8(21):5277-5282
pubmed: 28990781
Angew Chem Int Ed Engl. 2019 Aug 19;58(34):11681-11685
pubmed: 31218813
Chembiochem. 2020 Feb 17;21(4):451-460
pubmed: 31245902
Phys Chem Chem Phys. 2015 May 7;17(17):11144-9
pubmed: 25855020
Angew Chem Int Ed Engl. 2011 May 23;50(22):5070-4
pubmed: 21506223
J Phys Chem Lett. 2021 Apr 15;12(14):3679-3684
pubmed: 33829785
Sci Rep. 2019 Sep 10;9(1):13013
pubmed: 31506457
Angew Chem Int Ed Engl. 2020 Jun 8;59(24):9767-9772
pubmed: 32329172
J Magn Reson. 2017 Jul;280:117-126
pubmed: 28579097
J Magn Reson. 2020 Apr;313:106719
pubmed: 32217425