The nuclear lamina is required for proper development and nuclear shape distortion in tomato.
Leaf morphology
nuclear lamina
nuclear shape
nuclear size
root growth
tomato
Journal
Journal of experimental botany
ISSN: 1460-2431
Titre abrégé: J Exp Bot
Pays: England
ID NLM: 9882906
Informations de publication
Date de publication:
29 09 2023
29 09 2023
Historique:
received:
11
03
2023
accepted:
27
07
2023
medline:
5
10
2023
pubmed:
28
7
2023
entrez:
28
7
2023
Statut:
ppublish
Résumé
The nuclear lamina in plant cells is composed of plant-specific proteins, including nuclear matrix constituent proteins (NMCPs), which have been postulated to be functional analogs of lamin proteins that provide structural integrity to the organelle and help stabilize the three-dimensional organization of the genome. Using genomic editing, we generated alleles for the three genes encoding NMCPs in cultivated tomato (Solanum lycopersicum) to determine if the consequences of perturbing the nuclear lamina in this crop species were similar to or distinct from those observed in the model Arabidopsis thaliana. Loss of the sole NMCP2-class protein was lethal in tomato but is tolerated in Arabidopsis. Moreover, depletion of NMCP1-type nuclear lamina proteins leads to distinct developmental phenotypes in tomato, including leaf morphology defects and reduced root growth rate (in nmcp1b mutants), compared with cognate mutants in Arabidopsis. These findings suggest that the nuclear lamina interfaces with different developmental and signaling pathways in tomato compared with Arabidopsis. At the subcellular level, however, tomato nmcp mutants resembled their Arabidopsis counterparts in displaying smaller and more spherical nuclei in differentiated cells. This result argues that the plant nuclear lamina facilitates nuclear shape distortion in response to forces exerted on the organelle within the cell.
Identifiants
pubmed: 37503569
pii: 7232751
doi: 10.1093/jxb/erad294
pmc: PMC10540737
doi:
Substances chimiques
Plant Proteins
0
Nuclear Proteins
0
Nuclear Matrix-Associated Proteins
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.
Langues
eng
Sous-ensembles de citation
IM
Pagination
5500-5513Informations de copyright
© The Author(s) 2023. Published by Oxford University Press on behalf of the Society for Experimental Biology.
Références
Genome Biol. 2019 Apr 30;20(1):87
pubmed: 31039799
Plant Physiol. 2019 Apr;179(4):1315-1329
pubmed: 30696746
J Exp Bot. 2021 Sep 30;72(18):6190-6204
pubmed: 34086868
J Exp Bot. 2013 Apr;64(6):1553-64
pubmed: 23378381
Curr Opin Cell Biol. 2015 Feb;32:7-12
pubmed: 25460776
Science. 2012 Aug 17;337(6096):816-21
pubmed: 22745249
Dev Cell. 2022 Jan 10;57(1):19-31.e6
pubmed: 34822788
Trends Cell Biol. 2018 Jan;28(1):34-45
pubmed: 28893461
Nucleus. 2019 Dec;10(1):55-66
pubmed: 30879391
J Plant Physiol. 2019 Feb;233:20-30
pubmed: 30576929
Plant J. 2017 Nov;92(4):727-735
pubmed: 28873253
PLoS Biol. 2022 Oct 21;20(10):e3001831
pubmed: 36269771
Bioinformatics. 2015 Mar 1;31(5):767-9
pubmed: 25338722
BMC Evol Biol. 2013 Sep 30;13:214
pubmed: 24283922
Plant J. 2011 Mar;65(5):724-36
pubmed: 21251100
Development. 2008 Apr;135(7):1315-24
pubmed: 18305008
Dev Cell. 2013 Nov 11;27(3):345-52
pubmed: 24229646
Mol Biol Cell. 2000 Aug;11(8):2733-41
pubmed: 10930466
Methods Mol Biol. 2019;1917:171-182
pubmed: 30610636
Chromosome Res. 2014 Jun;22(2):241-52
pubmed: 24801343
J Clin Invest. 2004 Feb;113(3):370-8
pubmed: 14755334
Plant Physiol. 2014 Nov;166(3):1292-7
pubmed: 25225186
J Cell Biol. 2015 Jan 5;208(1):11-22
pubmed: 25559183
Front Plant Sci. 2021 Feb 17;12:645218
pubmed: 33679862
EMBO J. 1994 Jul 15;13(14):3378-88
pubmed: 7913892
Development. 2008 Apr;135(7):1325-34
pubmed: 18305007
Front Plant Sci. 2021 May 07;12:670306
pubmed: 34025705
Front Plant Sci. 2014 Feb 26;5:62
pubmed: 24616728
Nat Commun. 2020 Nov 20;11(1):5914
pubmed: 33219233
Genetics. 1993 Oct;135(2):575-88
pubmed: 7694886
Nucleus. 2017 Jan 2;8(1):46-59
pubmed: 27644504
Plant Cell. 2007 Sep;19(9):2793-803
pubmed: 17873096
Nat Plants. 2020 Jul;6(7):838-847
pubmed: 32601417
Chromosoma. 2010 Apr;119(2):195-204
pubmed: 19997923
Nature. 2003 May 15;423(6937):298-301
pubmed: 12748643
Methods Mol Biol. 2019;1864:225-234
pubmed: 30415340
Plant Physiol. 2001 Feb;125(2):615-26
pubmed: 11161019
Science. 2006 May 19;312(5776):1059-63
pubmed: 16645051
J Integr Plant Biol. 2016 Jul;58(7):669-78
pubmed: 26564029
Plant Cell Physiol. 2013 Apr;54(4):622-33
pubmed: 23396599
Mol Plant. 2017 Oct 9;10(10):1334-1348
pubmed: 28943325
Nucleus. 2018 Jan 1;9(1):119-124
pubmed: 29227210
Genomics Proteomics Bioinformatics. 2022 Feb;20(1):4-13
pubmed: 34487862
Plant Cell Rep. 1989 Mar;7(8):662-4
pubmed: 24240456
Plant Cell. 2000 Dec;12(12):2425-2440
pubmed: 11148288
J Biol Chem. 2007 Jul 6;282(27):20015-26
pubmed: 17488709
Nature. 2012 May 30;485(7400):635-41
pubmed: 22660326
Plant Cell. 2014 May 13;26(5):2143-2155
pubmed: 24824484
Science. 2013 Aug 30;341(6149):1240104
pubmed: 23990565
Ann Bot. 2019 Feb 15;123(3):521-532
pubmed: 30346473
BMC Plant Biol. 2013 Dec 05;13:200
pubmed: 24308514
Mol Biol Cell. 2018 Jan 15;29(2):220-233
pubmed: 29142071
Plant J. 2000 May;22(3):257-64
pubmed: 10849343
Plant J. 2009 Aug;59(3):499-508
pubmed: 19392710
Exp Cell Res. 1997 Apr 10;232(1):173-81
pubmed: 9141634
Proc Natl Acad Sci U S A. 2004 Jul 13;101(28):10428-33
pubmed: 15232008
Annu Rev Plant Biol. 2017 Apr 28;68:139-172
pubmed: 28226231
Mol Plant. 2017 Mar 6;10(3):530-532
pubmed: 28089950
Genes Genet Syst. 2005 Oct;80(5):345-50
pubmed: 16394585
Curr Opin Cell Biol. 2004 Feb;16(1):68-72
pubmed: 15037307
New Phytol. 2020 Jul;227(1):65-83
pubmed: 32129897
PLoS One. 2014 Mar 25;9(3):e93406
pubmed: 24667841
Annu Rev Biochem. 2015;84:131-64
pubmed: 25747401
Curr Biol. 2013 Sep 23;23(18):1776-81
pubmed: 23973298
Plant Physiol. 2008 May;147(1):128-42
pubmed: 18322146
Front Plant Sci. 2020 Jan 09;10:1639
pubmed: 31998332
Plant Physiol. 2011 Sep;157(1):29-39
pubmed: 21771915
Elife. 2013 Jan 29;2:e00471
pubmed: 23386978