Isothermal self-assembly of multicomponent and evolutive DNA nanostructures.
Journal
Nature nanotechnology
ISSN: 1748-3395
Titre abrégé: Nat Nanotechnol
Pays: England
ID NLM: 101283273
Informations de publication
Date de publication:
Nov 2023
Nov 2023
Historique:
received:
29
09
2022
accepted:
26
06
2023
medline:
20
11
2023
pubmed:
1
8
2023
entrez:
31
7
2023
Statut:
ppublish
Résumé
Thermal annealing is usually needed to direct the assembly of multiple complementary DNA strands into desired entities. We show that, with a magnesium-free buffer containing NaCl, complex cocktails of DNA strands and proteins can self-assemble isothermally, at room or physiological temperature, into user-defined nanostructures, such as DNA origamis, single-stranded tile assemblies and nanogrids. In situ, time-resolved observation reveals that this self-assembly is thermodynamically controlled, proceeds through multiple folding pathways and leads to highly reconfigurable nanostructures. It allows a given system to self-select its most stable shape in a large pool of competitive DNA strands. Strikingly, upon the appearance of a new energy minimum, DNA origamis isothermally shift from one initially stable shape to a radically different one, by massive exchange of their constitutive staple strands. This method expands the repertoire of shapes and functions attainable by isothermal self-assembly and creates a basis for adaptive nanomachines and nanostructure discovery by evolution.
Identifiants
pubmed: 37524905
doi: 10.1038/s41565-023-01468-2
pii: 10.1038/s41565-023-01468-2
pmc: PMC10656289
doi:
Substances chimiques
DNA
9007-49-2
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
1311-1318Subventions
Organisme : EC | EC Seventh Framework Programm | FP7 Ideas: European Research Council (FP7-IDEAS-ERC - Specific Programme: "Ideas" Implementing the Seventh Framework Programme of the European Community for Research, Technological Development and Demonstration Activities (2007 to 2013))
ID : 258782
Organisme : Agence Nationale de la Recherche (French National Research Agency)
ID : ANR-18-CE06-0019
Organisme : Agence Nationale de la Recherche (French National Research Agency)
ID : ANR-18-CE07-0001
Organisme : MEXT | Japan Society for the Promotion of Science (JSPS)
ID : Summer Program
Organisme : MEXT | Japan Society for the Promotion of Science (JSPS)
ID : Overseas Research Fellowship
Informations de copyright
© 2023. The Author(s).
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