Extracellular matrix remodelling in dental pulp tissue of carious human teeth through the prism of single-cell RNA sequencing.
Journal
International journal of oral science
ISSN: 2049-3169
Titre abrégé: Int J Oral Sci
Pays: India
ID NLM: 101504351
Informations de publication
Date de publication:
02 08 2023
02 08 2023
Historique:
received:
16
02
2023
accepted:
06
07
2023
revised:
27
06
2023
medline:
4
8
2023
pubmed:
3
8
2023
entrez:
2
8
2023
Statut:
epublish
Résumé
Carious lesions are bacteria-caused destructions of the mineralised dental tissues, marked by the simultaneous activation of immune responses and regenerative events within the soft dental pulp tissue. While major molecular players in tooth decay have been uncovered during the past years, a detailed map of the molecular and cellular landscape of the diseased pulp is still missing. In this study we used single-cell RNA sequencing analysis, supplemented with immunostaining, to generate a comprehensive single-cell atlas of the pulp of carious human teeth. Our data demonstrated modifications in the various cell clusters within the pulp of carious teeth, such as immune cells, mesenchymal stem cells (MSC) and fibroblasts, when compared to the pulp of healthy human teeth. Active immune response in the carious pulp tissue is accompanied by specific changes in the fibroblast and MSC clusters. These changes include the upregulation of genes encoding extracellular matrix (ECM) components, including COL1A1 and Fibronectin (FN1), and the enrichment of the fibroblast cluster with myofibroblasts. The incremental changes in the ECM composition of carious pulp tissues were further confirmed by immunostaining analyses. Assessment of the Fibronectin fibres under mechanical strain conditions showed a significant tension reduction in carious pulp tissues, compared to the healthy ones. The present data demonstrate molecular, cellular and biomechanical alterations in the pulp of human carious teeth, indicative of extensive ECM remodelling, reminiscent of fibrosis observed in other organs. This comprehensive atlas of carious human teeth can facilitate future studies of dental pathologies and enable comparative analyses across diseased organs.
Identifiants
pubmed: 37532703
doi: 10.1038/s41368-023-00238-z
pii: 10.1038/s41368-023-00238-z
pmc: PMC10397277
doi:
Substances chimiques
Fibronectins
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
30Informations de copyright
© 2023. The Author(s).
Références
Histochem J. 2000 Apr;32(4):247-52
pubmed: 10872890
Nat Rev Immunol. 2011 May;11(5):330-42
pubmed: 21508983
Am J Respir Crit Care Med. 2008 Mar 15;177(6):638-45
pubmed: 18096707
Eur Cell Mater. 2021 May 10;41:502-516
pubmed: 33970477
Am J Pathol. 2020 Oct;190(10):2123-2135
pubmed: 32650003
Nat Commun. 2021 Jul 19;12(1):4384
pubmed: 34282151
Cell. 2019 Jun 13;177(7):1888-1902.e21
pubmed: 31178118
Mol Aspects Med. 2019 Feb;65:2-15
pubmed: 29958900
iScience. 2020 Feb 21;23(2):100841
pubmed: 32058955
J Endod. 2017 Sep;43(9S):S17-S24
pubmed: 28778507
Cell Mol Life Sci. 2009 Jan;66(1):9-26
pubmed: 18726069
J Endod. 2020 Sep;46(9S):S26-S32
pubmed: 32950192
J Endod. 2014 Apr;40(4 Suppl):S46-51
pubmed: 24698693
Int Endod J. 2019 Mar;52(3):261-266
pubmed: 30724394
Am J Physiol Cell Physiol. 2007 Dec;293(6):C1934-46
pubmed: 17928541
Infect Immun. 2004 Jul;72(7):4102-8
pubmed: 15213155
Annu Rev Physiol. 2018 Feb 10;80:353-387
pubmed: 29433414
J Cell Sci. 2018 Mar 1;131(5):
pubmed: 29361522
Biochem Biophys Res Commun. 2003 Feb 7;301(2):495-501
pubmed: 12565889
J Endod. 2020 Sep;46(9S):S10-S18
pubmed: 32950180
J Cell Biol. 1994 Dec;127(6 Pt 2):2037-48
pubmed: 7806580
Nucleic Acids Res. 2019 Jul 2;47(W1):W191-W198
pubmed: 31066453
Genome Biol. 2019 Dec 23;20(1):296
pubmed: 31870423
Cell. 2021 Jun 24;184(13):3573-3587.e29
pubmed: 34062119
Mediators Inflamm. 2015;2015:230251
pubmed: 26538821
Semin Cell Dev Biol. 2022 Aug;128:130-136
pubmed: 35400564
Cell. 1999 Nov 12;99(4):351-4
pubmed: 10571177
Front Physiol. 2013 Nov 01;4:308
pubmed: 24198787
Exp Eye Res. 2016 Jan;142:56-70
pubmed: 26192991
Circulation. 2020 Nov 10;142(19):1831-1847
pubmed: 32972203
FASEB J. 2016 Aug;30(8):2651-61
pubmed: 27080639
Sci Rep. 2020 Sep 25;10(1):15808
pubmed: 32978500
J Endod. 2020 Sep;46(9S):S135-S142
pubmed: 32950185
Nat Commun. 2016 Jun 03;7:11703
pubmed: 27256716
J Cell Sci. 2016 Dec 1;129(23):4321-4327
pubmed: 27875272
Arch Oral Biol. 1989;34(5):307-13
pubmed: 2688611
Cell Adh Migr. 2015;9(1-2):48-82
pubmed: 25482829
J Leukoc Biol. 2003 Jun;73(6):689-701
pubmed: 12773501
J Am Soc Nephrol. 2017 Mar;28(3):785-801
pubmed: 27612995
Proc Natl Acad Sci U S A. 2022 Mar 29;119(13):e2120336119
pubmed: 35320046
Ann Rheum Dis. 2019 Oct;78(10):1379-1387
pubmed: 31405848
Matrix Biol Plus. 2020 Jul 17;8:100046
pubmed: 33543039
Nat Rev Immunol. 2009 Oct;9(10):692-703
pubmed: 19859064
Curr Biol. 2004 Nov 23;14(22):2011-8
pubmed: 15556863
Infect Immun. 2000 Dec;68(12):6785-9
pubmed: 11083796
J Biol Chem. 2005 Mar 4;280(9):7624-33
pubmed: 15611085
Am J Physiol Cell Physiol. 2020 Nov 1;319(5):C781-C796
pubmed: 32845719
Mol Biol Cell. 2004 Sep;15(9):4310-20
pubmed: 15240821
Commun Biol. 2021 Sep 2;4(1):1029
pubmed: 34475520
iScience. 2021 Apr 23;24(5):102405
pubmed: 33997688
Science. 2009 Nov 27;326(5957):1216-9
pubmed: 19965464
Sci Adv. 2021 Jul 28;7(31):
pubmed: 34321199
Methods Mol Biol. 2012;887:111-21
pubmed: 22566051
Arch Med Res. 2002 Sep-Oct;33(5):482-4
pubmed: 12459320
Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2002 Apr;93(4):474-82
pubmed: 12029288
BMC Immunol. 2011 Jan 24;12:9
pubmed: 21261944
J Endod. 2004 Feb;30(2):88-91
pubmed: 14977303
J Cell Physiol. 2007 Dec;213(3):565-73
pubmed: 17680633
Cytokine. 2003 May;22(3-4):84-8
pubmed: 12849707
Nat Commun. 2010;1:135
pubmed: 21139580
Cell Tissue Res. 2008 May;332(2):289-98
pubmed: 18305959
STAR Protoc. 2021 Nov 17;2(4):100953
pubmed: 34825216
Am J Med Genet A. 2006 Dec 1;140(23):2536-46
pubmed: 16955410
J Endod. 2007 Jun;33(6):643-51
pubmed: 17509400
Front Cell Dev Biol. 2020 Nov 30;8:605996
pubmed: 33330507
J Endod. 2020 Sep;46(9S):S46-S55
pubmed: 32950195