Long-term memory in Staphylococcus aureus α-hemolysin ion channel kinetics.
Approximate entropy
Detrended fluctuation analysis
Ion channel
Long-term correlation
α-Hemolysin
Journal
European biophysics journal : EBJ
ISSN: 1432-1017
Titre abrégé: Eur Biophys J
Pays: Germany
ID NLM: 8409413
Informations de publication
Date de publication:
Nov 2023
Nov 2023
Historique:
received:
20
06
2022
accepted:
20
07
2023
revised:
03
07
2023
medline:
28
11
2023
pubmed:
6
8
2023
entrez:
5
8
2023
Statut:
ppublish
Résumé
The kinetics of an ion channel are classically understood as a random process. However, studies have shown that in complex ion channels, formed by multiple subunits, this process can be deterministic, presenting long-term memory. Staphylococcus aureus α-hemolysin (α-HL) is a toxin that acts as the major factor in Staphylococcus aureus virulence. α-HL is a water-soluble protein capable of forming ion channels into lipid bilayers, by insertion of an amphipathic β-barrel. Here, the α-HL was used as an experimental model to study memory in ion channel kinetics. We applied the approximate entropy (ApEn) approach to analyze randomness and the Detrended Fluctuation Analysis (DFA) to investigate the existence of long memory in α-HL channel kinetics. Single-channel currents were measured through experiments with α-HL channels incorporated in planar lipid bilayers. All experiments were carried out under the following conditions: 1 M NaCl solution, pH 4.5; transmembrane potential of + 40 mV and temperature 25 ± 1 °C. Single-channel currents were recorded in real-time in the memory of a microcomputer coupled to an A/D converter and a patch-clamp amplifier. The conductance value of the α-HL channels was 0.82 ± 0.0025 nS (n = 128). The DFA analysis showed that the kinetics of α-HL channels presents long-term memory ([Formula: see text] = 0.63 ± 0.04). The ApEn outcomes showed low complexity to dwell times when open (ApEn
Identifiants
pubmed: 37542583
doi: 10.1007/s00249-023-01675-8
pii: 10.1007/s00249-023-01675-8
doi:
Substances chimiques
Hemolysin Proteins
0
Ion Channels
0
Lipid Bilayers
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
661-671Informations de copyright
© 2023. European Biophysical Societies' Association.
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