Endocytic myosin-1 is a force-insensitive, power-generating motor.
Journal
The Journal of cell biology
ISSN: 1540-8140
Titre abrégé: J Cell Biol
Pays: United States
ID NLM: 0375356
Informations de publication
Date de publication:
02 10 2023
02 10 2023
Historique:
received:
23
03
2023
revised:
17
05
2023
accepted:
24
07
2023
medline:
9
8
2023
pubmed:
7
8
2023
entrez:
7
8
2023
Statut:
ppublish
Résumé
Myosins are required for clathrin-mediated endocytosis, but their precise molecular roles in this process are not known. This is, in part, because the biophysical properties of the relevant motors have not been investigated. Myosins have diverse mechanochemical activities, ranging from powerful contractility against mechanical loads to force-sensitive anchoring. To better understand the essential molecular contribution of myosin to endocytosis, we studied the in vitro force-dependent kinetics of the Saccharomyces cerevisiae endocytic type I myosin called Myo5, a motor whose role in clathrin-mediated endocytosis has been meticulously studied in vivo. We report that Myo5 is a low-duty-ratio motor that is activated ∼10-fold by phosphorylation and that its working stroke and actin-detachment kinetics are relatively force-insensitive. Strikingly, the in vitro mechanochemistry of Myo5 is more like that of cardiac myosin than that of slow anchoring myosin-1s found on endosomal membranes. We, therefore, propose that Myo5 generates power to augment actin assembly-based forces during endocytosis in cells.
Identifiants
pubmed: 37549220
pii: 276159
doi: 10.1083/jcb.202303095
pmc: PMC10406613
pii:
doi:
Substances chimiques
Actins
0
Clathrin
0
Myosin Type I
EC 3.6.1.-
Myosins
EC 3.6.4.1
MYO5 protein, S cerevisiae
0
Saccharomyces cerevisiae Proteins
0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : NIGMS NIH HHS
ID : F32 GM142145
Pays : United States
Organisme : NIGMS NIH HHS
ID : R35 GM118149
Pays : United States
Organisme : NIGMS NIH HHS
ID : R37 GM057247
Pays : United States
Commentaires et corrections
Type : UpdateOf
Informations de copyright
© 2023 Pedersen et al.
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