Single-cell RNA sequencing in dissecting microenvironment of age-related macular degeneration: Challenges and perspectives.
Age-related macular degeneration
Microenvironment
Retina
Single-cell RNA sequencing
Transcriptome
Journal
Ageing research reviews
ISSN: 1872-9649
Titre abrégé: Ageing Res Rev
Pays: England
ID NLM: 101128963
Informations de publication
Date de publication:
09 2023
09 2023
Historique:
received:
16
09
2022
revised:
29
04
2023
accepted:
04
08
2023
medline:
7
9
2023
pubmed:
8
8
2023
entrez:
7
8
2023
Statut:
ppublish
Résumé
Age-related macular degeneration (AMD) is the leading cause of blindness in individuals over the age of 50 years, yet its etiology and pathogenesis largely remain uncovered. Single-cell RNA sequencing (scRNA-seq) technologies are recently developed and have a number of advantages over conventional bulk RNA sequencing techniques in uncovering the heterogeneity of complex microenvironments containing numerous cell types and cell communications during various biological processes. In this review, we summarize the latest discovered cellular components and regulatory mechanisms during AMD development revealed by scRNA-seq. In addition, we discuss the main challenges and future directions in exploring the pathophysiology of AMD equipped with single-cell technologies. Our review underscores the importance of multimodal single-cell platforms (such as single-cell spatiotemporal multi-omics and single-cell exosome omics) as new approaches for basic and clinical AMD research in identifying biomarker, characterizing cellular responses to drug treatment and environmental stimulation.
Identifiants
pubmed: 37549871
pii: S1568-1637(23)00189-7
doi: 10.1016/j.arr.2023.102030
pii:
doi:
Substances chimiques
Biomarkers
0
Types de publication
Journal Article
Review
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
102030Informations de copyright
Copyright © 2023 Elsevier B.V. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of Competing Interest The authors declare the following financial interests/personal relationships which may be considered as potential competing interests: Bo Qin reports financial support was provided by Natural Science Foundation of Guangdong Province, China. Bo Qin reports financial support was provided by Hunan Provincial Natural Science Foundation of China. Bo Qin reports financial support was provided by Shenzhen Science and Technology Innovation Committee, China. Bo Qin reports financial support was provided by Scientific research program of Xiangjiang Philanthropy Foundation. Bo Qin reports financial support was provided by Science Research Grant of Aier Eye Hospital Group. Dongcheng Liu reports financial support was provided by Shenzhen Science and Technology Innovation Committee, China. Dongcheng Liu reports financial support was provided by Science Research Grant of Aier Eye Hospital Group.