Identification and Quantification of Monosaccharides from Fungal Cell Walls and Exopolysaccharides by Gas Chromatography Coupled to Mass Spectrometry.
GC-MS
biofilm
cell wall
exopolysaccharide
monosaccharide
Journal
Current protocols
ISSN: 2691-1299
Titre abrégé: Curr Protoc
Pays: United States
ID NLM: 101773894
Informations de publication
Date de publication:
Aug 2023
Aug 2023
Historique:
medline:
10
8
2023
pubmed:
9
8
2023
entrez:
9
8
2023
Statut:
ppublish
Résumé
The fungal cell wall and secreted exopolysaccharides play an important role in the interactions between fungi and their environment. Despite their central role in fungal biology, ecology, and host-pathogen interactions, the composition of these polymers and their synthetic pathways are not well understood. The protocols presented in this article describe an approach to isolate fungal cell wall polysaccharides and to identify and quantify the monosaccharide composition of these polymers by gas chromatography-mass spectrometry (GC-MS). © 2023 The Authors. Current Protocols published by Wiley Periodicals LLC. Basic Protocol: O-methyl trimethylsilyl monosaccharide derivatives composition analysis by GC-MS Support Protocol: Fungal cell wall extraction.
Substances chimiques
Biopolymers
0
Fungal Polysaccharides
0
Monosaccharides
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
e853Informations de copyright
© 2023 The Authors. Current Protocols published by Wiley Periodicals LLC.
Références
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Gow, N. A. R., Latge, J. P., & Munro, C. A. (2017). The fungal cell wall: Structure, biosynthesis, and function. Microbiology Spectrum, 5(3), https://doi.org/10.1128/microbiolspec.funk-0035-2016
Gow, N. A. R., & Lenardon, M. D. (2023). Architecture of the dynamic fungal cell wall. Nature Reviews Microbiology, 21, 248-259. https://doi.org/10.1038/s41579-022-00796-9
Latgé, J.-P. (2020). The fungal cell wall: An armour and a weapon for human fungal pathogens (Vol. 425). Springer Nature.
le Mauff, F., & Sheppard, D. C. (2023). Understanding Aspergillus fumigatus galactosaminogalactan biosynthesis: A few questions remain. The Cell Surface, 9, 100095. https://doi.org/10.1016/j.tcsw.2023.100095
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