Genome-wide association study of resistance to anthracnose in pepper (Capsicum chinense) germplasm.
Anthracnose
Capsicum chinense
Colletotrichum spp.
GWAS
SNPs
Journal
BMC plant biology
ISSN: 1471-2229
Titre abrégé: BMC Plant Biol
Pays: England
ID NLM: 100967807
Informations de publication
Date de publication:
10 Aug 2023
10 Aug 2023
Historique:
received:
17
03
2023
accepted:
21
07
2023
medline:
14
8
2023
pubmed:
11
8
2023
entrez:
10
8
2023
Statut:
epublish
Résumé
Anthracnose is a fungal disease caused by Colletotrichum spp. that has a significant impact on worldwide pepper production. Colletotrichum scovillei is the most common pathogenic anthracnose-causing species in the Republic of Korea. The resistances of 197 pepper (Capsicum chinense) accessions deposited in Korea's National Agrobiodiversity Center were evaluated for their response against the virulent pathogens Colletotrichum acutatum isolate 'KSCa-1' and C. scovillei isolate 'Hana') in the field and in vitro methods for three consecutive years (2018 to 2020). The severity of the disease was recorded and compared between inoculation methods. Six phenotypically resistant pepper accessions were selected based on three years of disease data. All of the selected resistant pepper accessions outperformed the control resistant pepper in terms of resistance (PI 594,137). A genome-wide association study (GWAS) was carried out to identify single nucleotide polymorphisms (SNPs) associated with anthracnose resistance. An association analysis was performed using 53,518 SNPs and the disease score of the 2020 field and in vitro experiment results. Both field and in vitro experiments revealed 25 and 32 significantly associated SNPs, respectively. These SNPs were found on all chromosomes except Ch06 and Ch07 in the field experiment, whereas in the in vitro experiment they were found on all chromosomes except Ch04 and Ch11. In this study, six resistant C. chinense accessions were selected. Additionally, in this study, significantly associated SNPs were found in a gene that codes for a protein kinase receptor, such as serine/threonine-protein kinase, and other genes that are known to be involved in disease resistance. This may strengthen the role of these genes in the development of anthracnose resistance in Capsicum spp. As a result, the SNPs discovered to be strongly linked in this study can be used to identify a potential marker for selecting pepper material resistant to anthracnose, which will assist in the development of resistant varieties.
Sections du résumé
BACKGROUND
BACKGROUND
Anthracnose is a fungal disease caused by Colletotrichum spp. that has a significant impact on worldwide pepper production. Colletotrichum scovillei is the most common pathogenic anthracnose-causing species in the Republic of Korea.
RESULTS
RESULTS
The resistances of 197 pepper (Capsicum chinense) accessions deposited in Korea's National Agrobiodiversity Center were evaluated for their response against the virulent pathogens Colletotrichum acutatum isolate 'KSCa-1' and C. scovillei isolate 'Hana') in the field and in vitro methods for three consecutive years (2018 to 2020). The severity of the disease was recorded and compared between inoculation methods. Six phenotypically resistant pepper accessions were selected based on three years of disease data. All of the selected resistant pepper accessions outperformed the control resistant pepper in terms of resistance (PI 594,137). A genome-wide association study (GWAS) was carried out to identify single nucleotide polymorphisms (SNPs) associated with anthracnose resistance. An association analysis was performed using 53,518 SNPs and the disease score of the 2020 field and in vitro experiment results. Both field and in vitro experiments revealed 25 and 32 significantly associated SNPs, respectively. These SNPs were found on all chromosomes except Ch06 and Ch07 in the field experiment, whereas in the in vitro experiment they were found on all chromosomes except Ch04 and Ch11.
CONCLUSION
CONCLUSIONS
In this study, six resistant C. chinense accessions were selected. Additionally, in this study, significantly associated SNPs were found in a gene that codes for a protein kinase receptor, such as serine/threonine-protein kinase, and other genes that are known to be involved in disease resistance. This may strengthen the role of these genes in the development of anthracnose resistance in Capsicum spp. As a result, the SNPs discovered to be strongly linked in this study can be used to identify a potential marker for selecting pepper material resistant to anthracnose, which will assist in the development of resistant varieties.
Identifiants
pubmed: 37563545
doi: 10.1186/s12870-023-04388-4
pii: 10.1186/s12870-023-04388-4
pmc: PMC10413807
doi:
Substances chimiques
Protein Kinases
EC 2.7.-
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
389Subventions
Organisme : National Institute of Agricultural Sciences, RDA, Republic of Korea.
ID : PJ01604012023 and PJ013251022020
Organisme : National Institute of Agricultural Sciences, RDA, Republic of Korea.
ID : PJ01604012023 and PJ013251022020
Organisme : National Institute of Agricultural Sciences, RDA, Republic of Korea.
ID : PJ01604012023 and PJ013251022020
Organisme : National Institute of Agricultural Sciences, RDA, Republic of Korea.
ID : PJ01604012023 and PJ013251022020
Organisme : National Institute of Agricultural Sciences, RDA, Republic of Korea.
ID : PJ01604012023 and PJ013251022020
Organisme : National Institute of Agricultural Sciences, RDA, Republic of Korea.
ID : PJ01604012023 and PJ013251022020
Organisme : National Institute of Agricultural Sciences, RDA, Republic of Korea.
ID : PJ01604012023 and PJ013251022020
Organisme : National Institute of Agricultural Sciences, RDA, Republic of Korea.
ID : PJ01604012023 and PJ013251022020
Organisme : National Institute of Agricultural Sciences, RDA, Republic of Korea.
ID : PJ01604012023 and PJ013251022020
Organisme : National Institute of Agricultural Sciences, RDA, Republic of Korea.
ID : PJ01604012023 and PJ013251022020
Organisme : National Institute of Agricultural Sciences, RDA, Republic of Korea.
ID : PJ01604012023 and PJ013251022020
Organisme : National Institute of Agricultural Sciences, RDA, Republic of Korea.
ID : PJ01604012023 and PJ013251022020
Organisme : National Institute of Agricultural Sciences, RDA, Republic of Korea.
ID : PJ01604012023 and PJ013251022020
Organisme : National Institute of Agricultural Sciences, RDA, Republic of Korea.
ID : PJ01604012023 and PJ013251022020
Informations de copyright
© 2023. BioMed Central Ltd., part of Springer Nature.
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