Engineered coumarin accumulation reduces mycotoxin-induced oxidative stress and disease susceptibility.
coumarin
disease resistance
mycotoxin
reactive oxygen species
soybean
stress tolerance
Journal
Plant biotechnology journal
ISSN: 1467-7652
Titre abrégé: Plant Biotechnol J
Pays: England
ID NLM: 101201889
Informations de publication
Date de publication:
Dec 2023
Dec 2023
Historique:
revised:
23
06
2023
received:
20
09
2022
accepted:
23
07
2023
medline:
17
11
2023
pubmed:
14
8
2023
entrez:
14
8
2023
Statut:
ppublish
Résumé
Coumarins can fight pathogens and are thus promising for crop protection. Their biosynthesis, however, has not yet been engineered in crops. We tailored the constitutive accumulation of coumarins in transgenic Nicotiana benthamiana, Glycine max and Arabidopsis thaliana plants, as well as in Nicotiana tabacum BY-2 suspension cells. We did so by overexpressing A. thaliana feruloyl-CoA 6-hydroxylase 1 (AtF6'H1), encoding the key enzyme of scopoletin biosynthesis. Besides scopoletin and its glucoside scopolin, esculin at low level was the only other coumarin detected in transgenic cells. Mechanical damage of scopolin-accumulating tissue led to a swift release of scopoletin, presumably from the scopolin pool. High scopolin levels in A. thaliana roots coincided with reduced susceptibility to the root-parasitic nematode Heterodera schachtii. In addition, transgenic soybean plants were more tolerant to the soil-borne pathogenic fungus Fusarium virguliforme. Because mycotoxin-induced accumulation of reactive oxygen species and cell death were reduced in the AtF6'H1-overexpressors, the weaker sensitivity to F. virguliforme may be caused by attenuated oxidative damage of coumarin-hyperaccumulating cells. Together, engineered coumarin accumulation is promising for enhanced disease resilience of crops.
Identifiants
pubmed: 37578146
doi: 10.1111/pbi.14144
pmc: PMC10651151
doi:
Substances chimiques
coumarin
A4VZ22K1WT
Scopoletin
KLF1HS0SXJ
Mycotoxins
0
Coumarins
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
2490-2506Subventions
Organisme : BASF Plant Science Company GmbH
Organisme : Deutsche Forschungsgemeinschaft
ID : CO 186/10-1
Informations de copyright
© 2023 The Authors. Plant Biotechnology Journal published by Society for Experimental Biology and The Association of Applied Biologists and John Wiley & Sons Ltd.
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