Simulation of Microgravity and Coculturing with Hematopoietic Cells Oppositely Modulate Wnt Signaling in Mesenchymal Stromal Cells.
Wnt signaling
mesenchymal stromal cells (MSCs)
simulated microgravity
Journal
Doklady. Biochemistry and biophysics
ISSN: 1608-3091
Titre abrégé: Dokl Biochem Biophys
Pays: United States
ID NLM: 101126895
Informations de publication
Date de publication:
Jun 2023
Jun 2023
Historique:
received:
15
12
2022
accepted:
15
01
2023
revised:
11
01
2023
medline:
17
8
2023
pubmed:
16
8
2023
entrez:
15
8
2023
Statut:
ppublish
Résumé
The osteogenic potential of mesenchymal stromal cells (MSCs) can determine bone homeostasis and the physical characteristics of bones. Microgravity reduces the ability of these cells to differentiate in osteogenic direction. It has been shown that the addition of hematopoietic stem and progenitor cells (HSPCs) to MSC culture in vitro can have the opposite effect. The aim of this study was to identify transcriptional changes in 84 genes associated with Wnt signaling in MSCs during microgravity simulation and interaction with HSPCs. The results indicate an increase in the non-canonical Wnt signaling activity during coculturing of MSCs and HSPCs, while simulated microgravity enhances the canonical component of this signaling pathway. These changes may underlie the modulation of osteogenic potential of MSCs in hematopoietic niche under microgravity.
Identifiants
pubmed: 37582870
doi: 10.1134/S1607672923700187
pii: 10.1134/S1607672923700187
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
95-98Informations de copyright
© 2023. Pleiades Publishing, Ltd.
Références
Buravkova, L.B., Gershovich, P.M., Gershovich, J.G., et al., Mechanisms of gravitational sensitivity of osteogenic precursor cells, Acta Nat., 2010, vol. 2, no. 1 (4), pp. 28–35.
Man, J., Graham, T., Squires-Donelly, G., et al., The effects of microgravity on bone structure and function, npj Microgravity, 2022, vol. 8, no. 1, pp. 1–15.
Andreeva, E.R., Ezdakova, M.I., Bobyleva, P.I., et al., Osteogenic commitment of MSC is enhanced after interaction with umbilical cord blood mononuclear cells in vitro, Bull Exp. Biol. Med., 2021, vol. 171, no. 4, pp. 541–546.
doi: 10.1007/s10517-021-05266-5
pubmed: 34542768
Jia, Y., Zhang, C., Zheng, X., et al., Co-cultivation of progenitor cells enhanced osteogenic gene expression and angiogenesis potential in vitro, J. Int. Med. Res., 2021, vol. 49, no. 4, p. 03000605211004024.
Yang, X., Sun, L.W., Liang, M., et al., The response of Wnt/β-catenin signaling pathway in osteocytes under simulated microgravity, Microgravity Sci. Technol., 2015, vol. 27, no. 6, pp. 473–483.
doi: 10.1007/s12217-015-9439-8
Houschyar, K.S., Tapking, C., Borrelli, M.R., et al., Wnt pathway in bone repair and regeneration—what do we know so far, Front. Cell Dev. Biol., 2019, vol. 6, p. 170.
doi: 10.3389/fcell.2018.00170
pubmed: 30666305
pmcid: 6330281
Takam, KamgaP., Bazzoni, R., Dal, ColloG., et al., The role of notch and Wnt signaling in msc communication in Normal and leukemic bone marrow niche, Front. Cell Dev. Biol., 2021, vol. 8, p. 599276.
Dominici, M., Le Blanc, K., Mueller, I., et al., Minimal criteria for defining multipotent mesenchymal stromal cells. The International Society for Cellular Therapy position statement, Cytotherapy, 2006, vol. 8, no. 4, pp. 315–317.
doi: 10.1080/14653240600855905
pubmed: 16923606
Livak, K.J. and Schmittgen, T.D., Analysis of relative gene expression data using real-time quantitative PCR and the 2
doi: 10.1006/meth.2001.1262
pubmed: 11846609
Gu, Q., Tian, H., Zhang, K., et al., Wnt5a/FZD4 mediates the mechanical stretch-induced osteogenic differentiation of bone mesenchymal stem cells, Cell. Physiol. Biochem., 2018, vol. 48, no. 1, pp. 215–226.
doi: 10.1159/000491721
pubmed: 30007964
Yang, X., Sun, L.W., Liang, M., et al., The response of Wnt/β-catenin signaling pathway in osteocytes under simulated microgravity, Microgravity Sci. Technol., 2015, vol. 27, no. 6, pp. 473–483.
doi: 10.1007/s12217-015-9439-8
Jothimani, G., Di Liddo, R., Pathak, S., et al., Wnt signaling regulates the proliferation potential and lineage commitment of human umbilical cord derived mesenchymal stem cells, Mol. Biol. Rep., 2020, vol. 47, no. 2, pp. 1293–1308.
doi: 10.1007/s11033-019-05232-5
pubmed: 31853765
Albers, J., Keller, J., Baranowsky, A., et al., Canonical Wnt signaling inhibits osteoclastogenesis independent of osteoprotegerin, J. Cell Biol., 2013, vol. 200, no. 4, pp. 537–549.
doi: 10.1083/jcb.201207142
pubmed: 23401003
pmcid: 3575535
Ono, M., Inkson, C.A., Kilts, T.M., et al., WISP-1/CCN4 regulates osteogenesis by enhancing BMP-2 activity, J. Bone Miner. Res., 2011, vol. 26, no. 1, pp. 193–208.
doi: 10.1002/jbmr.205
pubmed: 20684029