Yeast lacking the sterol C-5 desaturase Erg3 are tolerant to the anti-inflammatory triterpenoid saponin escin.
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
21 08 2023
21 08 2023
Historique:
received:
10
05
2023
accepted:
08
08
2023
medline:
23
8
2023
pubmed:
22
8
2023
entrez:
21
8
2023
Statut:
epublish
Résumé
Escin is a mixture of over 30 glycosylated triterpenoid (saponin) structures, extracted from the dried fruit of horse chestnuts. Escin is currently used as an anti-inflammatory, and has potential applications in the treatment of arthritis and cancer. Engineered yeast would enable production of specific bioactive components of escin at industrial scale, however many saponins have been shown to be toxic to yeast. Here we report that a Saccharomyces cerevisiae strain specifically lacking the sterol C-5 desaturase gene ERG3, exhibits striking enhanced tolerance to escin treatment. Transcriptome analyses, as well as pre-mixing of escin with sterols, support the hypothesis that escin interacts directly with ergosterol, but not as strongly with the altered sterols present in erg3Δ. A diverse range of saponins are of commercial interest, and this research highlights the value of screening lipidome mutants to identify appropriate hosts for engineering the industrial production of saponins.
Identifiants
pubmed: 37604855
doi: 10.1038/s41598-023-40308-0
pii: 10.1038/s41598-023-40308-0
pmc: PMC10442444
doi:
Substances chimiques
Escin
6805-41-0
Saponins
0
Sterols
0
Anti-Inflammatory Agents
0
Fatty Acid Desaturases
EC 1.14.19.-
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
13617Subventions
Organisme : Biotechnology and Biological Sciences Research Council
ID : BB/S017712/1
Pays : United Kingdom
Informations de copyright
© 2023. Springer Nature Limited.
Références
Front Plant Sci. 2021 Oct 07;12:727882
pubmed: 34691107
Annu Rev Plant Biol. 2014;65:225-57
pubmed: 24498976
Plant Physiol. 2012 Dec;160(4):1881-95
pubmed: 23027665
Nat Commun. 2016 Nov 07;7:13324
pubmed: 27819292
Int J Pharm. 2021 Jun 15;603:120709
pubmed: 33992714
Front Plant Sci. 2019 Nov 28;10:1448
pubmed: 31850004
Sci Signal. 2014 Sep 16;7(343):re7
pubmed: 25227612
Pharmacol Res. 2001 Sep;44(3):183-93
pubmed: 11529685
Nat Prod Chem Res. 2016 Apr;3(4):
pubmed: 27213168
Molecules. 2019 Dec 27;25(1):
pubmed: 31892278
Antimicrob Agents Chemother. 2006 Aug;50(8):2732-40
pubmed: 16870766
Drug Des Devel Ther. 2019 Sep 27;13:3425-3437
pubmed: 31631970
J Chem Biol. 2008 Nov;1(1-4):63-77
pubmed: 19568799
Proc Natl Acad Sci U S A. 2010 Mar 2;107(9):4170-5
pubmed: 20150508
Mol Biol Cell. 2009 Apr;20(7):2083-95
pubmed: 19225153
Biochem Biophys Res Commun. 1995 Feb 27;207(3):910-5
pubmed: 7864896
Nature. 2002 Aug 22;418(6900):889-92
pubmed: 12192413
Sci Rep. 2019 Apr 3;9(1):5542
pubmed: 30944386
EMBO J. 2001 Aug 1;20(15):4035-40
pubmed: 11483507
Biochim Biophys Acta. 2007 Aug;1773(8):1311-40
pubmed: 17604854
Phytochemistry. 2019 May;161:41-74
pubmed: 30818173
Mol Biol Cell. 1999 Nov;10(11):3943-57
pubmed: 10564282
Yeast. 2013 Apr;30(4):129-44
pubmed: 23447388
FEMS Microbiol Rev. 2010 Nov;34(6):952-85
pubmed: 20412306
Biochem Soc Trans. 2002 Aug;30(4):698-702
pubmed: 12196168
J Fungi (Basel). 2017 Dec 21;4(1):
pubmed: 29371494
Biophys J. 2004 Nov;87(5):3264-76
pubmed: 15315952
Sci Rep. 2018 Oct 11;8(1):15168
pubmed: 30310096
PLoS One. 2016 Oct 11;11(10):e0164365
pubmed: 27727329
Curr Genet. 2015 Nov;61(4):503-11
pubmed: 25957506
J Bacteriol. 1993 May;175(10):2853-8
pubmed: 8491706
Mol Cell Biol. 2005 Aug;25(16):7375-85
pubmed: 16055745
Proc Natl Acad Sci U S A. 1999 Oct 26;96(22):12923-8
pubmed: 10536024
J Cell Biol. 2008 Feb 25;180(4):813-26
pubmed: 18299351
Biomolecules. 2020 Oct 23;10(11):
pubmed: 33114062
Mol Biol Cell. 2008 Aug;19(8):3323-33
pubmed: 18508925
Plants (Basel). 2021 Oct 15;10(10):
pubmed: 34685998
Nat Chem Biol. 2014 May;10(5):400-6
pubmed: 24681535
J Bacteriol. 2002 Jan;184(1):250-65
pubmed: 11741867
J Fungi (Basel). 2021 Oct 25;7(11):
pubmed: 34829190
Mol Biotechnol. 2010 Sep;46(1):94-104
pubmed: 20204713
Biochim Biophys Acta. 1992 Oct 5;1110(2):127-36
pubmed: 1390841
J Biol Chem. 2010 May 7;285(19):14823-33
pubmed: 20231268
Front Pharmacol. 2019 Mar 22;10:280
pubmed: 30967782
Mol Biol Cell. 2000 Dec;11(12):4241-57
pubmed: 11102521
FEMS Microbiol Rev. 2001 Jan;25(1):125-45
pubmed: 11152943
Science. 1999 Aug 6;285(5429):901-6
pubmed: 10436161
J Mol Biol. 2017 Feb 17;429(4):531-542
pubmed: 28077284
Cancer Lett. 2018 May 28;422:1-8
pubmed: 29474858
Crit Rev Biochem Mol Biol. 2014 Nov-Dec;49(6):439-62
pubmed: 25286183
Langmuir. 2017 Oct 31;33(43):12351-12361
pubmed: 28985678
Free Radic Biol Med. 2009 Sep 15;47(6):880-9
pubmed: 19559787
Microb Cell Fact. 2005 Nov 10;4:31
pubmed: 16283927
Mol Biol Cell. 2002 Aug;13(8):2664-80
pubmed: 12181337
Phytochemistry. 2007 Feb;68(3):275-97
pubmed: 17141815
Int Immunopharmacol. 2016 Feb;31:257-65
pubmed: 26773773
Mol Cell Biol. 2001 Oct;21(19):6395-405
pubmed: 11533229
Trends Biochem Sci. 1999 Nov;24(11):437-40
pubmed: 10542411
Appl Microbiol Biotechnol. 2006 Feb;69(6):704-10
pubmed: 16010571
Med Mycol. 2015 Nov;53(8):845-51
pubmed: 26092104
Mol Biol Cell. 2001 Feb;12(2):323-37
pubmed: 11179418
Phys Chem Chem Phys. 2018 Apr 4;20(14):9070-9083
pubmed: 29505043
Proc Natl Acad Sci U S A. 2009 Feb 17;106(7):2136-41
pubmed: 19174513
Nucleic Acids Res. 2018 Jan 4;46(D1):D586-D594
pubmed: 29045755
Genes (Basel). 2020 Jul 15;11(7):
pubmed: 32679672
Phytochemistry. 2011 Apr;72(6):435-57
pubmed: 21333312
J Agric Food Chem. 2022 Jun 8;70(22):6573-6590
pubmed: 35621387
Yeast. 2020 Jan;37(1):27-44
pubmed: 31800968