Enhanced production of N-acetyl-glucosaminidase by marine Aeromonas caviae CHZ306 in bioreactor.
Aeromonas caviae
Bioproduction
Bioreactor
Chitinase
N-Acetyl-glucosaminidase
Journal
Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology]
ISSN: 1678-4405
Titre abrégé: Braz J Microbiol
Pays: Brazil
ID NLM: 101095924
Informations de publication
Date de publication:
Sep 2023
Sep 2023
Historique:
received:
31
05
2022
accepted:
31
07
2023
pmc-release:
23
08
2024
medline:
11
9
2023
pubmed:
23
8
2023
entrez:
23
8
2023
Statut:
ppublish
Résumé
N-Acetyl-glucosaminidases (GlcNAcases) are exoenzymes found in a wide range of living organisms, which have gained great attention in the treatment of disorders related to diabetes, Alzheimer's, Tay-Sachs', and Sandhoff's diseases; the control of phytopathogens; and the synthesis of bioactive GlcNAc-containing products. Aiming at future industrial applications, in this study, GlcNAcase production by marine Aeromonas caviae CHZ306 was enhanced first in shake flasks in terms of medium composition and then in bench-scale stirred-tank bioreactor in terms of physicochemical conditions. Stoichiometric balance between the bioavailability of carbon and nitrogen in the formulated culture medium, as well as the use of additional carbon and nitrogen sources, played a central role in improving the bioprocess, considerably increasing the enzyme productivity. The optimal cultivation medium was composed of colloidal α-chitin, corn steep liquor, peptone A, and mineral salts, in a 5.2 C:N ratio. Optimization of pH, temperature, colloidal α-chitin concentration, and k
Identifiants
pubmed: 37610567
doi: 10.1007/s42770-023-01088-x
pii: 10.1007/s42770-023-01088-x
pmc: PMC10485184
doi:
Substances chimiques
Carbon
7440-44-0
Chitin
1398-61-4
Hexosaminidases
EC 3.2.1.-
Nitrogen
N762921K75
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
1533-1545Subventions
Organisme : Fundação de Amparo à Pesquisa do Estado de São Paulo
ID : 2012/16824-0
Organisme : Fundação de Amparo à Pesquisa do Estado de São Paulo
ID : 2013/18773-6
Informations de copyright
© 2023. The Author(s) under exclusive licence to Sociedade Brasileira de Microbiologia.
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