Chromosome-level genome assembly of Babesia caballi reveals diversity of multigene families among Babesia species.

Animals Horses Babesia / genetics Phylogeny Multigene Family Open Reading Frames Chromosomes
Babesia caballi Comparative genomics Equine babesiosis Multigene expansion

Journal

BMC genomics
ISSN: 1471-2164
Titre abrégé: BMC Genomics
Pays: England
ID NLM: 100965258

Informations de publication

Date de publication:
24 Aug 2023
Historique:
received: 14 01 2023
accepted: 27 07 2023
medline: 28 8 2023
pubmed: 25 8 2023
entrez: 24 8 2023
Statut: epublish

Résumé

Babesia caballi is an intraerythrocytic parasite from the phylum Apicomplexa, capable of infecting equids and causing equine piroplasmosis. However, since there is limited genome information available on B. caballi, molecular mechanisms involved in host specificity and pathogenicity of this species have not been fully elucidated yet. Genomic DNA from a B. caballi subclone was purified and sequenced using both Illumina and Nanopore technologies. The resulting assembled sequence consisted of nine contigs with a size of 12.9 Mbp, rendering a total of 5,910 protein-coding genes. The phylogenetic tree of Apicomplexan species was reconstructed using 263 orthologous genes. We identified 481 ves1-like genes and named "ves1c". In contrast, expansion of the major facilitator superfamily (mfs) observed in closely related B. bigemina and B. ovata species was not found in B. caballi. A set of repetitive units containing an open reading frame with a size of 297 bp was also identified. We present a chromosome-level genome assembly of B. caballi. Our genomic data may contribute to estimating gene expansion events involving multigene families and exploring the evolution of species from this genus.

Sections du résumé

BACKGROUND BACKGROUND
Babesia caballi is an intraerythrocytic parasite from the phylum Apicomplexa, capable of infecting equids and causing equine piroplasmosis. However, since there is limited genome information available on B. caballi, molecular mechanisms involved in host specificity and pathogenicity of this species have not been fully elucidated yet.
RESULTS RESULTS
Genomic DNA from a B. caballi subclone was purified and sequenced using both Illumina and Nanopore technologies. The resulting assembled sequence consisted of nine contigs with a size of 12.9 Mbp, rendering a total of 5,910 protein-coding genes. The phylogenetic tree of Apicomplexan species was reconstructed using 263 orthologous genes. We identified 481 ves1-like genes and named "ves1c". In contrast, expansion of the major facilitator superfamily (mfs) observed in closely related B. bigemina and B. ovata species was not found in B. caballi. A set of repetitive units containing an open reading frame with a size of 297 bp was also identified.
CONCLUSIONS CONCLUSIONS
We present a chromosome-level genome assembly of B. caballi. Our genomic data may contribute to estimating gene expansion events involving multigene families and exploring the evolution of species from this genus.

Identifiants

DOI: 10.1186/s12864-023-09540-w PMID: 37620766 PMC: PMC10463595
pubmed: 37620766
doi: 10.1186/s12864-023-09540-w
pii: 10.1186/s12864-023-09540-w
pmc: PMC10463595
doi:

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

483

Subventions

Organisme : the Japan Racing Association
ID : 2-3272

Informations de copyright

© 2023. BioMed Central Ltd., part of Springer Nature.

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Auteurs

Akihiro Ochi (A)

Equine Research Institute, Japan Racing Association, Shimotsuke, Tochigi, Japan.

Taishi Kidaka (T)

International Institute for Zoonosis Control, Hokkaido University, Sapporo, Hokkaido, Japan.

Hassan Hakimi (H)

National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido, Japan.
Department of Veterinary Pathobiology, School of Veterinary Medicine and Biomedical Sciences, Texas A&M University, College Station, Texas, USA.

Masahito Asada (M)

National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido, Japan.

Junya Yamagishi (J)

International Institute for Zoonosis Control, Hokkaido University, Sapporo, Hokkaido, Japan. junya@czc.hokudai.ac.jp.
Global Station for Zoonosis Control, GI-CoRE, Hokkaido University, Sapporo, Hokkaido, Japan. junya@czc.hokudai.ac.jp.

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Classifications MeSH

questionsmedicales.fr › Eucaryotes › Animaux › Chromosome-level genome assembly of Babesia...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Perissodactyla › Equidae › Equus caballus › Chromosome-level genome assembly of Babesia...
questionsmedicales.fr › Eucaryotes › Alveolata › Apicomplexa › Piroplasmia › Piroplasmida › Babesia › Chromosome-level genome assembly of Babesia...
questionsmedicales.fr › Sciences de l'information › Phylogenèse › Chromosome-level genome assembly of Babesia...
questionsmedicales.fr › Phénomènes génétiques › Structures génétiques › Génome › Composants de génome › Gènes › Famille multigénique › Chromosome-level genome assembly of Babesia...
questionsmedicales.fr › Phénomènes génétiques › Structures génétiques › Génome › Composants de génome › Gènes › Composants de gène › Cadres ouverts de lecture › Chromosome-level genome assembly of Babesia...
questionsmedicales.fr › Phénomènes génétiques › Structures génétiques › Chromosomes › Chromosome-level genome assembly of Babesia...