A novel binding site between the voltage-dependent calcium channel Ca
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
26 08 2023
26 08 2023
Historique:
received:
24
04
2023
accepted:
23
08
2023
medline:
28
8
2023
pubmed:
27
8
2023
entrez:
26
8
2023
Statut:
epublish
Résumé
We developed a new method to analyze protein-protein interactions using a dual-inducible prokaryotic expression system. To evaluate protein-protein binding, a chimeric fusion toxin gene was constructed using a DNase-treated short DNA fragment (epitope library) and CcdB, which encodes a DNA topoisomerase II toxin. Protein-protein interactions would affect toxin activity, resulting in colony formation. Using this novel system, we found a new binding site in the voltage-dependent calcium channel α1 subunit (Ca
Identifiants
pubmed: 37634019
doi: 10.1038/s41598-023-41168-4
pii: 10.1038/s41598-023-41168-4
pmc: PMC10460381
doi:
Substances chimiques
Epitopes
0
Calcium Channels
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
13986Informations de copyright
© 2023. Springer Nature Limited.
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