Soft rot of postharvest pepper: bacterial pathogen, pathogenicity and its biological control using Lactobacillus farciminis LJLAB1.
Lactobacillus farciminis
disease control
pathogenicity
pepper
soft rot
Journal
Journal of the science of food and agriculture
ISSN: 1097-0010
Titre abrégé: J Sci Food Agric
Pays: England
ID NLM: 0376334
Informations de publication
Date de publication:
15 Jan 2024
15 Jan 2024
Historique:
revised:
14
08
2023
received:
25
03
2023
accepted:
28
08
2023
medline:
20
11
2023
pubmed:
28
8
2023
entrez:
28
8
2023
Statut:
ppublish
Résumé
Soft rot is the most important bacterial disease of postharvest pepper during storage and transportation. The main objectives of this study were to investigate the bacterial pathogen species causing pepper soft rot and seek for an antagonistic bacterium to control this disease. Pathogens Pectobacterium carotovorum, Enterobacter sp., Klebsiella sp., Pseudomonas sp. and Bacillus sp. were verified to be the causes of soft rot which were isolated from rotten peppers. Among them, P. carotovorum had the highest prevalence, including P. carotovorum subsp. carotovorum (Pcc) and P. carotovorum subsp. brasilisesis (Pcb). The result of pathogenicity analysis showed that Pcb Jm2 had strong pathogenicity at 25 °C even at a cell concentration of 10 L. farciminis LJLAB1 can be an effective biological control agent to control pepper soft rot caused by Pcb. © 2023 Society of Chemical Industry.
Sections du résumé
BACKGROUND
BACKGROUND
Soft rot is the most important bacterial disease of postharvest pepper during storage and transportation. The main objectives of this study were to investigate the bacterial pathogen species causing pepper soft rot and seek for an antagonistic bacterium to control this disease.
RESULTS
RESULTS
Pathogens Pectobacterium carotovorum, Enterobacter sp., Klebsiella sp., Pseudomonas sp. and Bacillus sp. were verified to be the causes of soft rot which were isolated from rotten peppers. Among them, P. carotovorum had the highest prevalence, including P. carotovorum subsp. carotovorum (Pcc) and P. carotovorum subsp. brasilisesis (Pcb). The result of pathogenicity analysis showed that Pcb Jm2 had strong pathogenicity at 25 °C even at a cell concentration of 10
CONCLUSION
CONCLUSIONS
L. farciminis LJLAB1 can be an effective biological control agent to control pepper soft rot caused by Pcb. © 2023 Society of Chemical Industry.
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
443-455Subventions
Organisme : This research was supported by the Project of Sichuan Science and Technology Plan, China (2021YFQ0071), the Project of Chongqing Science and Technology Bureau, China (cstc2021jscx-cylhX0015), the Fundamental Research Funds for the Central Universities (SWU-KT22047) and National Natural Science Foundation of China (No. 32102032)
Informations de copyright
© 2023 Society of Chemical Industry.
Références
Ling L, Feng S, Tu Y, Yang C, Jiang K, Ma W et al., Preservation activity of Artemisia essential oils and a monomer in treating pepper bacterium and fungus diseases. J Food Processing Preserv 45:e15501 (2021).
Muangkote S, Vichitsoonthonkul T, Srilaong V, Wongs-Aree C and Photchanachai S, Influence of roasting on chemical profile, antioxidant and antibacterial activities of dried chili. Food Sci Biotechnol 28:303-310 (2019).
Abd-El-Khair H, Abdel-Gaied TG, Mikhail MS, Abdel-Alim AI and El-Nasr H, Biological control of Pectobacterium carotovorum subsp. carotovorum, the causal agent of bacterial soft rot in vegetables, in vitro and in vivo tests. Bull Natl Res Centre 45:37 (2021).
Jeon HS, Lokos L, Han KS, Ryu JA and Hur JS, Isolation of lichen-forming fungi from Hungarian lichens and their antifungal activity against fungal pathogens of hot pepper anthracnose. Plant Pathol J 25:38-46 (2009).
Gurung S, Screening of Endophytes for the Control of Root Rot Pathogens of Pepper (Capsicum annum). State University, Academic Information Services, Nashville (2016) Available: http://digitalscholarship.tnstate.edu/dissertations/AAI10241419.
Waleron M, Waleron K and Lojkowska E, Characterization of Pectobacterium carotovorum subsp. odoriferum causing soft rot of stored vegetables. Eur J Plant Pathol 139:457-469 (2014).
Glasner J, Marquez-Villavicencio M, Kim H-S, Jahn C, Ma B, Biehl B et al., Niche-specificity and the variable fraction of the Pectobacterium pan-genome. Mol Plant Microbe Interact 21:1549-1560 (2008).
Mashavha ML, Characterisation of Pectobacterium carotovorum subsp. brasiliense isolates causing blackleg and soft rot diseases of potato in South Africa. University of Pretoria, Theses and Dissertations (2013). Aviliable: http://hdl.handle.net/2263/41108.
Raybaudi Massilia RM, Mosqueda MJ and Martin BO, Antimicrobial activity of essential oils on Salmonella enteritidis, Escherichia coli, and Listeria innocua in fruit juices. J Food Prot 69:1579-1586 (2006).
Sharma M, Patel JR, Conway WS, Ferguson S and Sulakvelidze A, Effectiveness of bacteriophages in reducing Escherichia coli O157:H7 on fresh-cut cantaloupes and lettuces. J Food Prot 72:1481-1485 (2009).
Pinto G, Almeida C and Azeredo J, Bacteriophages to control Shiga toxin-producing E. coli: safety and regulatory challenges. Crit Rev Biotechnol 40:1081-1097 (2020).
Zhang X, Li B, Zhang Z, Chen Y and Tian S, Antagonistic yeasts: a promising alternative to chemical fungicides for controlling postharvest decay of fruit. J Fungi-Open Access Mycology J 6:158 (2020).
Chen PH, Chen RY and Chou JY, Screening and evaluation of yeast antagonists for biological control of Botrytis cinerea on strawberry fruits. Mycobiology 46:33-46 (2018).
Ghanbari M, Jami M, Domig KJ and Kneifel W, Seafood biopreservation by lactic acid bacteria: a review. LWT Food Sci Technol 54:315-324 (2013).
Qi T, Wang S, Deng L, Yi L and Zeng K, Controlling pepper soft rot by Lactobacillus paracasei WX322 and identification of multiple bacteriocins by complete genome sequencing. Food Control 121:107629 (2021).
Voidarou C, Alexopoulos A, Tsinas A, Rozos G, Tzora A, Skoufos I et al., Effectiveness of bacteriocin-producing lactic acid bacteria and Bifidobacterium isolated from honeycombs against spoilage microorganisms and pathogens isolated from fruits and vegetables. Appl Sci 10:7309 (2020).
Yi L, Qi T, Ma J and Zeng K, Genome and metabolites analysis reveal insights into control of foodborne pathogens in fresh-cut fruits by Lactobacillus pentosus MS031 isolated from Chinese Sichuan Paocai. Postharvest Biol Technol 164:111150 (2020).
Matei GM, Matei S, Matei A, Cornea CP and Jerca IO, Bioprotection of fresh food products against blue mold using lactic acid bacteria with antifungal properties. 21:11201-11208 (2016).
Reuter G, Lactobacillus alimentarius sp. nov., nom rev. and Lactobacillus farciminis sp. nov., nom. rev. Syst Appl Microbiol 4:277-279 (1983).
Maes E, Sadovskaya I, Lévêque M, Elass-Rochard E, Payré B, Grard T et al., Structure and biological activities of a hexosamine-rich cell wall polysaccharide isolated from the probiotic Lactobacillus farciminis. Glycoconj J 36:39-55 (2019).
Ait-Belgnaoui A, Han W, Lamine F, Eutamene H, Fioramonti J, Bueno L et al., Lactobacillus farciminis treatment suppresses stress induced visceral hypersensitivity: a possible action through interaction with epithelial cell cytoskeleton contraction. Gut 55:1090-1094 (2006).
Chiou TY, Oshima K, Suda W, Hattori M and Takahashi T, Draft genome sequence of Lactobacillus farciminis NBRC 111452, isolated from Kôso, a Japanese sugar-vegetable fermented beverage. Genome Announc 4:e01514-e01515 (2015).
Bampidis V, Azimonti G, Bastos M, Christensen H, Usemund D, Durjava MK et al., Safety and efficacy of Sorbiflore ADVANCE (Lactobacillus rhamnosus CNCM I-3698 and Lactobacillus farciminis CNCM I-3699) as a feed additive for weaned piglets. EFSA J 18:e06081 (2020).
Authority E, Safety and efficacy of the product Sorbiflore, a preparation of Lactobacillus rhamnosus and Lactobacillus farciminis, as feed additive for piglets: scientific opinion of the panel on additives and products or substances used in animal feed. EFSA J 771:1-3 (2008).
Bin L, Wei-wen X, Ze-li G, Si-yu L, Qiu-nan Z, Xue-qun P et al., Water loss and pericarp browning of litchi (Litchi chinensis) and longan (Dimocarpus longan) fruit maintain seed vigor. Sci Hortic 290:110519 (2021).
Li X, Fu L, Chen C, Sun W, Tian Y and Xie H, Characteristics and rapid diagnosis of Pectobacterium carotovorum ssp. associated with bacterial soft rot of vegetables in China. Plant Disease 104:1158-1166 (2020).
Yi L, Qi T, Hong Y, Deng L and Zeng K, Screening of bacteriocin-producing lactic acid bacteria in Chinese homemade pickle and dry-cured meat, and bacteriocin identification by genome sequencing. LWT Food Sci Technol 125:109177 (2020).
Lü X, Yi L, Dang J, Dang Y and Liu B, Purification of novel bacteriocin produced by Lactobacillus coryniformis MXJ 32 for inhibiting bacterial foodborne pathogens including antibiotic-resistant microorganisms. Food Control 46:264-271 (2014).
Lahiri D, Chakraborti S, Jasu A, Nag M, Dutta B, Dash S et al., Production and purification of bacteriocin from Leuconostoc lactis SM 2 strain. Biocatal Agric Biotechnol 30:101845 (2020).
Yi L, Zeng P, Liu J, Wong KY and Chen S, Antimicrobial peptide zp37 inhibits Escherichia coli O157:H7 in alfalfa sprouts by inflicting damage in cell membrane and binding to DNA. LWT Food Sci Technol 146:111392 (2021).
Shu P, Min D, Ai W, Li J and Guo Y, L-arginine treatment attenuates postharvest decay and maintains quality of strawberry fruit by promoting nitric oxide synthase pathway. Postharvest Biol Technol 168:111253 (2020).
Li L, Yuan L, Shi Y, Xie X, Chai A, Wang Q et al., Comparative genomic analysis of Pectobacterium carotovorum subsp. brasiliense SX309 provides novel insights into its genetic and phenotypic features. BMC Genomics 20:1-17 (2019).
Chatkaew A and Kim J, Correlations among disease severity, firmness, minerals, and cell wall composition in radish (Raphanus sativus L.) and baemoochae (×Brassicoraphanus) roots in relation to tissue maceration by Pectobacterium carotovorum. Hortic Environ Biotechnol 54:346-356 (2013).
Hua W, The membrane-lipid peroxidation in the process of development and senescence of freesia flowers. Acta Univ Agric Boreali-Occidentalis 2:72-74 (1994).
Martínez-Castellanos G, Pelayo-Zaldívar C, Pérez-Flores L, López-Luna A, Gimeno M, Bárzana E et al., Postharvest litchi (Litchi chinensis Sonn.) quality preservation by Lactobacillus plantarum. Postharvest Biol Technol 59:172-178 (2011).
Davidsson PR, Tarja K, Outi N and Palva ET, Pathogenicity of and plant immunity to soft rot pectobacteria. Front Plant Sci 4:191 (2013).
Nishijima K, Keith RC, Fitch MM, Wall MM, Sugiyama LS and Nishijima WT, Production of internal yellowing symptoms on resistant and susceptible papaya cultivars by Enterobacter cloacae at varying inoculum concentrations. Phytopathology 97:S84 (2007).
Wu J, Ding Z, Ying D and Hu Z, First report on Enterobacter sp. causing soft rot of Amorphophallus konjac in China. J General Plant Pathol 77:312-314 (2011).
Elhafeez EA, Alkhazindar M and Sayed E, Isolation and characterization of Enterobacter strains causing potato soft rot disease in Egypt. Minia Sci Bull Botany Sect 29:1-3 (2017).
Fan HC, Zeng L, Yang PW, Guo ZX and Bai TT, First report of banana soft rot caused by Klebsiella variicola in China. Plant Disease 100:517 (2016).
Chandrashekar BS, Prasannakumar MK, Puneeth ME, Teli K and Desai RU, First report of bacterial soft rot of carrot caused by Klebsiella variicola in India. New Disease Rep 37:21 (2018).
Yi YK and Hyeoksoo S, Soft rot of red-pepper fruit caused by Pseudomonas fluorescens biovar II. Plant Disease Res 6:1-5 (2000).
Godfrey SAC and Marshall JW, Identification of cold-tolerant Pseudomonas viridiflava and P. marginalis causing severe carrot postharvest bacterial soft rot during refrigerated export from New Zealand. Plant Pathol 51:155-162 (2010).
Zhang C, Lin T, Li J, Ma G, Wang Y, Zhu P et al., First report of the melon stem rot disease in protected cultivation caused by Pseudomonas fluorescens. J Plant Diseases Prot 123:247-255 (2016).
Elbanna K, Elnaggar S and Bakeer A, Characterization of Bacillus altitudinis as a new causative agent of bacterial soft rot. J Phytopathol 162:712-722 (2014).
Peng Q, Yuan Y and Gao M, Bacillus pumilus, a novel ginger rhizome rot pathogen in China. Plant Disease 97:1308-1315 (2013).
Vidal E, Montesano, Denecke and Palva, cell wall-degrading enzymes from Erwinia carotovora cooperate in the salicylic acid-independent induction of a plant defense response. Mol Plant Microbe Interact 11:23-32 (1998).
Lee DH, Lim JA, Lee J, Roh E, Jung K, Choi M et al., Characterization of genes required for the pathogenicity of Pectobacterium carotovorum subsp. carotovorum Pcc21 in Chinese cabbage. Microbiology 159:1487-1496 (2013).
Yang Z, Cramer C and Lacy G, Erwinia carotovora subsp. carotovora pectic enzymes: in planta gene activation and roles in soft-rot pathogenesis. Mol Plant Microbe Interact 5:104-112 (1992).
Perombelon M and Kelman A, Ecology of the soft rot Erwinias. Annu Rev Phytopathol 18:361-387 (1980).
Barras F, Extracellular enzymes and pathogenesis of soft-rot Erwinia. Annu Rev Phytopathol 32:201-234 (1994).
Rafiqi M, Ellis JG, Ludowici VA, Hardham AR and Dodds PN, Challenges and progress towards understanding the role of effectors in plant-fungal interactions. Curr Opin Plant Biol 15:477-482 (2012).
Huang C, Hou J, Huang M, Hu M, Deng L, Zeng K et al., A comprehensive review of segment drying (vesicle granulation and collapse) in citrus fruit: current state and future directions. Sci Hortic 309:111683 (2023).