Application of one-step inner-recycling counter-current chromatography for the preparative separation and purification of chemical constituents from the rhizome of Bergenia ciliate (haw.) Sternb.
counter-current chromatography
one-step inner-recycling mode
rhizome of Bergenia ciliate (haw.) sternb
separation method
similar partition coefficient compounds
Journal
Journal of separation science
ISSN: 1615-9314
Titre abrégé: J Sep Sci
Pays: Germany
ID NLM: 101088554
Informations de publication
Date de publication:
Oct 2023
Oct 2023
Historique:
revised:
04
08
2023
received:
03
05
2023
accepted:
10
08
2023
medline:
30
10
2023
pubmed:
1
9
2023
entrez:
1
9
2023
Statut:
ppublish
Résumé
Bergenia ciliata (haw.) Sternb, the renowned pharmaceutical plant in Jammu and Kashmir of Pakistan, is widely applied in treating different illnesses including diabetes, diarrhea, and vomiting. This work employed an efficient one-step inner-recycling counter-current chromatography for preparative separating and purifying compounds with similar partition coefficients from the rhizome of Bergenia ciliate (haw.). Five compounds, including quercetin rhamnodiglucoside (1), quercetin-3-O-rutinoside (2), bergenine (3), kaempferol (4), and palmatic acid (5), were successfully separated using the optimized biphasic solvent system that contained ter-butylmetylether/n-butanol/acetonitrile/water (2:2:1:5, v/v) with the purities over 98%. Mass spectrometry and nuclear magnetic resonance were conducted for structural identification. As a result, our proposed strategy might be applied in separating compounds with similar partition coefficients, which was advantageous with regard to the less solvent and time consumption, and the increased number of theoretical plates.
Identifiants
pubmed: 37654052
doi: 10.1002/jssc.202300306
doi:
Substances chimiques
Plant Extracts
0
Solvents
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
e2300306Subventions
Organisme : Natural Science Foundation of Shandong Province
ID : ZR2020MH396
Organisme : post-doctoral fellowship and Science, Education, Industry Integration Innovation Pilot Project
ID : 2022JBZ02-06
Organisme : post-doctoral fellowship and Science, Education, Industry Integration Innovation Pilot Project
ID : 2022PX029
Organisme : University-Local IUR Collaborative Innovation Fund Project
ID : 2020-CXY16
Organisme : Qilu University of Technology (Shandong Academy of Science)
Organisme : National Talented Young Scientists Programme (TYSP)
Organisme : National Natural Science Foundation of China
ID : 32200311
Informations de copyright
© 2023 Wiley-VCH GmbH.
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