Countercurrent chromatography separation of vitamin E isomers in a co-current mode.
co-current mode
countercurrent chromatography
tocopherol
tocotrienol
vitamin E
Journal
Journal of separation science
ISSN: 1615-9314
Titre abrégé: J Sep Sci
Pays: Germany
ID NLM: 101088554
Informations de publication
Date de publication:
Nov 2023
Nov 2023
Historique:
revised:
18
07
2023
received:
26
04
2023
accepted:
15
08
2023
medline:
9
11
2023
pubmed:
1
9
2023
entrez:
1
9
2023
Statut:
ppublish
Résumé
Vitamin E represents a group of lipophilic phenolic compounds, including α-tocopherol, β-tocopherol, γ-tocopherol, and δ-tocopherol, and α-tocotrienol, β-tocotrienol, γ-tocotrienol, and δ-tocotrienol isomers. Different forms of vitamin E have been proven to exhibit varying biological activities. However, due to their structural similarities, the separation of vitamin E isomers is a challenging task. Therefore, it is crucial to establish an efficient method for isolating individual isomers. In this study, co-current countercurrent chromatography was employed to isolate vitamin E isomers from commercial capsules using a n-heptane-methanol-water (10:9.5:0.5, v/v) solvent system. The partition coefficients of the main constituents in the capsules ranged from 0.94 to 6.23, requiring over 450 min for a complete separation. To improve separation efficiency, a co-current elution mode was implemented and the flow rates of the two liquid phases as well as sample amount were examined. The results suggested that increasing the flow rate of the stationary phase and sample size could result in more effective separation, shorter separation time, and higher yield. It proved that co-current countercurrent chromatography was an effective method for the separation of vitamin E isomers.
Identifiants
pubmed: 37654055
doi: 10.1002/jssc.202300285
doi:
Substances chimiques
Vitamin E
1406-18-4
Solvents
0
Methanol
Y4S76JWI15
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
e2300285Subventions
Organisme : National Natural Science Foundation of China
ID : 31800289
Organisme : Fundamental Research Fund for the Central Universities
ID : Z109021803
Informations de copyright
© 2023 Wiley-VCH GmbH.
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