Activation of oxidoreductases by the formation of enzyme assembly.
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
01 09 2023
01 09 2023
Historique:
received:
03
07
2023
accepted:
31
08
2023
medline:
4
9
2023
pubmed:
2
9
2023
entrez:
1
9
2023
Statut:
epublish
Résumé
Biological properties of protein molecules depend on their interaction with other molecules, and enzymes are no exception. Enzyme activities are controlled by their interaction with other molecules in living cells. Enzyme activation and their catalytic properties in the presence of different types of polymers have been studied in vitro, although these studies are restricted to only a few enzymes. In this study, we show that addition of poly-l-lysine (PLL) can increase the enzymatic activity of multiple oxidoreductases through formation of enzyme assemblies. Oxidoreductases with an overall negative charge, such as l-lactate oxidase, d-lactate dehydrogenase, pyruvate oxidase, and acetaldehyde dehydrogenase, each formed assemblies with the positively charged PLL via electrostatic interactions. The enzyme activities of these oxidoreductases in the enzyme assemblies were several-folds higher than those of the enzyme in their natural dispersed state. In the presence of PLL, the turnover number (k
Identifiants
pubmed: 37658129
doi: 10.1038/s41598-023-41789-9
pii: 10.1038/s41598-023-41789-9
pmc: PMC10474089
doi:
Substances chimiques
L-Lactate Dehydrogenase
EC 1.1.1.27
Lysine
K3Z4F929H6
Poly A
24937-83-5
Polymers
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
14381Informations de copyright
© 2023. Springer Nature Limited.
Références
Microorganisms. 2022 Jan 21;10(2):
pubmed: 35208686
Mol Cell. 2015 Mar 5;57(5):936-947
pubmed: 25747659
Chem Commun (Camb). 2022 Dec 1;58(96):13317-13320
pubmed: 36342014
Proc Natl Acad Sci U S A. 2016 May 24;113(21):5958-63
pubmed: 27166422
Biotechnol Adv. 2021 Dec;53:107868
pubmed: 34774927
Proc Natl Acad Sci U S A. 2021 Aug 10;118(32):
pubmed: 34341111
Elife. 2020 Apr 16;9:
pubmed: 32298230
Curr Opin Struct Biol. 2017 Dec;47:140-150
pubmed: 29035814
Biophys J. 2021 Apr 6;120(7):1170-1186
pubmed: 32853565
Nat Chem Biol. 2021 Jun;17(6):693-702
pubmed: 34035521
Mol Cell. 2022 Oct 6;82(19):3693-3711.e10
pubmed: 36108633
Curr Opin Biotechnol. 2002 Aug;13(4):345-51
pubmed: 12323357
Biotechnol Adv. 2015 Nov 15;33(7):1443-54
pubmed: 25747291
Org Biomol Chem. 2023 Jan 4;21(2):306-314
pubmed: 36342388
Biochim Biophys Acta. 1964 Apr 4;86:194-7
pubmed: 14166862
Appl Environ Microbiol. 2015 Jun 15;81(12):4098-110
pubmed: 25862219
Langmuir. 2014 Apr 8;30(13):3826-31
pubmed: 24635224
Mol Biol Cell. 2020 Jun 1;31(12):1201-1205
pubmed: 32463766
Plant J. 2020 Sep;103(6):2168-2177
pubmed: 32656814
Nat Commun. 2021 Nov 1;12(1):6293
pubmed: 34725341
Nat Commun. 2020 May 8;11(1):2284
pubmed: 32385250
J Biol Chem. 2017 Sep 22;292(38):15838-15848
pubmed: 28784664
ACS Sens. 2023 Jun 23;8(6):2368-2374
pubmed: 37216270
Biochemistry. 2005 Apr 5;44(13):5086-94
pubmed: 15794646
Sci Rep. 2023 Jan 25;13(1):1435
pubmed: 36697449
J Biol Chem. 1986 Oct 25;261(30):14178-83
pubmed: 3771528
Trends Endocrinol Metab. 2015 Dec;26(12):746-757
pubmed: 26520658