Condensation of STM is critical for shoot meristem maintenance and salt tolerance in Arabidopsis.

Salt Tolerance / genetics Arabidopsis / genetics Meristem / genetics Salt Stress Cell Differentiation Homeodomain Proteins Arabidopsis Proteins / genetics
KNOX1 axillary meristem biomolecular condensate intrinsically disordered proteins salt stress shoot apical meristem

Journal

Molecular plant
ISSN: 1752-9867
Titre abrégé: Mol Plant
Pays: England
ID NLM: 101465514

Informations de publication

Date de publication:
04 09 2023
Historique:
received: 11 01 2023
revised: 20 05 2023
accepted: 04 09 2023
medline: 21 9 2023
pubmed: 7 9 2023
entrez: 7 9 2023
Statut: ppublish

Résumé

The shoot meristem generates the entire shoot system and is precisely maintained throughout the life cycle under various environmental challenges. In this study, we identified a prion-like domain (PrD) in the key shoot meristem regulator SHOOT MERISTEMLESS (STM), which distinguishes STM from other related KNOX1 proteins. We demonstrated that PrD stimulates STM to form nuclear condensates, which are required for maintaining the shoot meristem. STM nuclear condensate formation is stabilized by selected PrD-containing STM-interacting BELL proteins in vitro and in vivo. Moreover, condensation of STM promotes its interaction with the Mediator complex subunit MED8 and thereby enhances its transcriptional activity. Thus, condensate formation emerges as a novel regulatory mechanism of shoot meristem functions. Furthermore, we found that the formation of STM condensates is enhanced upon salt stress, which allows enhanced salt tolerance and increased shoot branching. Our findings highlight that the transcription factor partitioning plays an important role in cell fate determination and might also act as a tunable environmental acclimation mechanism.

Identifiants

DOI: 10.1016/j.molp.2023.09.005 PMID: 37674313
pubmed: 37674313
pii: S1674-2052(23)00257-5
doi: 10.1016/j.molp.2023.09.005
pii:
doi:

Substances chimiques

STM protein, Arabidopsis 0
Homeodomain Proteins 0
Arabidopsis Proteins 0

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

1445-1459

Informations de copyright

Copyright © 2023 The Author. Published by Elsevier Inc. All rights reserved.

Auteurs

Xiuwei Cao (X)

State Key Laboratory of Plant Genomics and National Center for Plant Gene Research (Beijing), Institute of Genetics and Developmental Biology, The Innovative Academy of Seed Design, Chinese Academy of Sciences, Beijing 100101, China.

Qingwei Du (Q)

State Key Laboratory of Plant Genomics and National Center for Plant Gene Research (Beijing), Institute of Genetics and Developmental Biology, The Innovative Academy of Seed Design, Chinese Academy of Sciences, Beijing 100101, China; College of Life Sciences, University of Chinese Academy of Sciences, Beijing 100049, China; Beijing Agro-Biotechnology Research Center, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, China.

Yahe Guo (Y)

State Key Laboratory of Protein and Plant Gene Research, School of Life Sciences, Peking University, Beijing 100871, China.

Ying Wang (Y)

College of Life Sciences, University of Chinese Academy of Sciences, Beijing 100049, China. Electronic address: yingwang@ucas.ac.cn.

Yuling Jiao (Y)

State Key Laboratory of Plant Genomics and National Center for Plant Gene Research (Beijing), Institute of Genetics and Developmental Biology, The Innovative Academy of Seed Design, Chinese Academy of Sciences, Beijing 100101, China; State Key Laboratory of Protein and Plant Gene Research, School of Life Sciences, Peking University, Beijing 100871, China; Peking-Tsinghua Center for Life Sciences, Center for Quantitative Biology, Academy for Advanced Interdisciplinary Studies, Peking University, Beijing 100871, China; Peking University Institute of Advanced Agricultural Sciences, Shandong Laboratory of Advanced Agricultural Sciences in Weifang, Weifang, Shandong 261325, China; College of Advanced Agricultural Sciences, University of Chinese Academy of Sciences, Beijing 100049, China. Electronic address: yuling.jiao@pku.edu.cn.

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Classifications MeSH

questionsmedicales.fr › Phénomènes biologiques › Adaptation biologique › Adaptation physiologique › Acclimatation › Tolérance au sel › Condensation of STM is critical for shoot...
questionsmedicales.fr › Eucaryotes › Viridiplantae › Streptophyta › Embryophyta › Tracheobionta › Magnoliopsida › Brassicaceae › Arabidopsis › Condensation of STM is critical for shoot...
questionsmedicales.fr › Structures de plante › Racines de plante › Méristème › Condensation of STM is critical for shoot...
questionsmedicales.fr › Phénomènes physiologiques › Stress physiologique › Stress salin › Condensation of STM is critical for shoot...
questionsmedicales.fr › Phénomènes physiologiques cellulaires › Différenciation cellulaire › Condensation of STM is critical for shoot...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines de liaison à l'ADN › Protéines à homéodomaine › Condensation of STM is critical for shoot...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines végétales › Protéines d'Arabidopsis › Condensation of STM is critical for shoot...