Functionally discrete fine roots differ in microbial assembly, microbial functional potential, and produced metabolites.
microbial functions
rhizoplane
rhizosphere
root microbiome
root order
root physiology
spatial colonization
Journal
Plant, cell & environment
ISSN: 1365-3040
Titre abrégé: Plant Cell Environ
Pays: United States
ID NLM: 9309004
Informations de publication
Date de publication:
Dec 2023
Dec 2023
Historique:
revised:
12
07
2023
received:
19
01
2023
accepted:
18
08
2023
medline:
3
11
2023
pubmed:
7
9
2023
entrez:
7
9
2023
Statut:
ppublish
Résumé
Traditionally, fine roots were grouped using arbitrary size categories, rarely capturing the heterogeneity in physiology, morphology and functionality among different fine root orders. Fine roots with different functional roles are rarely separated in microbiome-focused studies and may result in confounding microbial signals and host-filtering across different root microbiome compartments. Using a 26-year-old common garden, we sampled fine roots from four temperate tree species that varied in root morphology and sorted them into absorptive and transportive fine roots. The rhizoplane and rhizosphere were characterized using 16S rRNA gene and internal transcribed spacer region amplicon sequencing and shotgun metagenomics for the rhizoplane to identify potential microbial functions. Fine roots were subject to metabolomics to spatially characterize resource availability. Both fungi and bacteria differed according to root functional type. We observed additional differences between the bacterial rhizoplane and rhizosphere compartments for absorptive but not transportive fine roots. Rhizoplane bacteria, as well as the root metabolome and potential microbial functions, differed between absorptive and transportive fine roots, but not the rhizosphere bacteria. Functional differences were driven by sugar transport, peptidases and urea transport. Our data highlights the importance of root function when examining root-microbial relationships, emphasizing different host selective pressures imparted on different root microbiome compartments.
Substances chimiques
RNA, Ribosomal, 16S
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
3919-3932Subventions
Organisme : USDA National Institute of Food and Agriculture
Organisme : DOE Biological and Environmental Research program
Organisme : DOE Environmental Molecular Sciences Laboratory
Organisme : NSF Centre for Research on Programmable Plant Systems
Informations de copyright
© 2023 The Authors. Plant, Cell & Environment published by John Wiley & Sons Ltd.
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