Plant immunity suppression by an exo-β-1,3-glucanase and an elongation factor 1α of the rice blast fungus.


Journal

Nature communications
ISSN: 2041-1723
Titre abrégé: Nat Commun
Pays: England
ID NLM: 101528555

Informations de publication

Date de publication:
07 09 2023
Historique:
received: 27 05 2021
accepted: 23 08 2023
medline: 11 9 2023
pubmed: 8 9 2023
entrez: 7 9 2023
Statut: epublish

Résumé

Fungal cell walls undergo continual remodeling that generates β-1,3-glucan fragments as products of endo-glycosyl hydrolases (GHs), which can be recognized as pathogen-associated molecular patterns (PAMPs) and trigger plant immune responses. How fungal pathogens suppress those responses is often poorly understood. Here, we study mechanisms underlying the suppression of β-1,3-glucan-triggered plant immunity by the blast fungus Magnaporthe oryzae. We show that an exo-β-1,3-glucanase of the GH17 family, named Ebg1, is important for fungal cell wall integrity and virulence of M. oryzae. Ebg1 can hydrolyze β-1,3-glucan and laminarin into glucose, thus suppressing β-1,3-glucan-triggered plant immunity. However, in addition, Ebg1 seems to act as a PAMP, independent of its hydrolase activity. This Ebg1-induced immunity appears to be dampened by the secretion of an elongation factor 1 alpha protein (EF1α), which interacts and co-localizes with Ebg1 in the apoplast. Future work is needed to understand the mechanisms behind Ebg1-induced immunity and its suppression by EF1α.

Identifiants

pubmed: 37679340
doi: 10.1038/s41467-023-41175-z
pii: 10.1038/s41467-023-41175-z
pmc: PMC10484928
doi:

Substances chimiques

Peptide Elongation Factor 1 0

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

5491

Informations de copyright

© 2023. Springer Nature Limited.

Références

Eukaryot Cell. 2015 Sep;14(9):846-57
pubmed: 25934689
Planta. 2005 Sep;222(1):107-17
pubmed: 15968510
Annu Rev Phytopathol. 2007;45:437-56
pubmed: 17489691
Proc Natl Acad Sci U S A. 2009 Dec 29;106(52):22522-7
pubmed: 20007779
Nature. 2021 Aug;596(7873):583-589
pubmed: 34265844
Proc Natl Acad Sci U S A. 2007 Jul 17;104(29):12217-22
pubmed: 17626179
Clin Rev Allergy Immunol. 2018 Apr;54(2):213-223
pubmed: 28251581
Plant Cell. 2000 May;12(5):817-26
pubmed: 10810152
Eukaryot Cell. 2010 Aug;9(8):1294-8
pubmed: 20543062
Plant Cell. 2019 Jan;31(1):172-188
pubmed: 30610168
Mol Microbiol. 2009 Aug;73(4):553-70
pubmed: 19602150
PLoS One. 2015 Aug 11;10(8):e0135239
pubmed: 26263509
Fungal Biol. 2013 Oct;117(10):673-81
pubmed: 24119405
Appl Environ Microbiol. 2010 Oct;76(19):6583-90
pubmed: 20709852
Curr Opin Plant Biol. 2009 Aug;12(4):406-13
pubmed: 19616468
Trends Microbiol. 2017 Dec;25(12):957-967
pubmed: 28641930
J Bacteriol. 1979 Sep;139(3):924-31
pubmed: 113390
J Gen Microbiol. 1993 Feb;139(2):325-34
pubmed: 8436950
Nat Plants. 2019 Nov;5(11):1167-1176
pubmed: 31636399
Mol Plant Pathol. 2019 Dec;20(12):1710-1721
pubmed: 31603622
FEMS Microbiol Lett. 2002 Apr 23;210(1):81-5
pubmed: 12023081
Gene. 1997 Oct 1;198(1-2):289-96
pubmed: 9370294
Nucleic Acids Res. 2022 Jan 7;50(D1):D1522-D1527
pubmed: 34871441
Mol Plant Microbe Interact. 2006 Dec;19(12):1420-30
pubmed: 17153926
Biosci Biotechnol Biochem. 2001 Jun;65(6):1310-4
pubmed: 11471729
Nucleic Acids Res. 2019 Jan 8;47(D1):D1211-D1217
pubmed: 30252093
Gene. 1991 Jan 15;97(2):173-82
pubmed: 1900250
Plant Physiol. 1989 Jan;89(1):138-43
pubmed: 16666504
PLoS Genet. 2012;8(8):e1002869
pubmed: 22876203
Proc Natl Acad Sci U S A. 2006 Jul 18;103(29):11086-91
pubmed: 16829581
Plant Physiol. 2019 Apr;179(4):1416-1430
pubmed: 30696749
Mol Microbiol. 2005 Jun;56(6):1675-88
pubmed: 15916615
Plant Cell. 2015 Jul;27(7):2057-72
pubmed: 26163574
Genomics Proteomics Bioinformatics. 2022 Aug;20(4):728-746
pubmed: 34284133
Microbiology (Reading). 1998 Nov;144 ( Pt 11):3079-3086
pubmed: 9846753
Plant J. 2020 Jun;102(6):1142-1156
pubmed: 31925978
Glycobiology. 2014 May;24(5):418-27
pubmed: 24429506
Nature. 2007 May 3;447(7140):92-6
pubmed: 17450126
Mol Cells. 2011 Dec;32(6):561-9
pubmed: 22058019
Nature. 2005 Apr 21;434(7036):980-6
pubmed: 15846337
Immunity. 2003 Sep;19(3):311-5
pubmed: 14499107
Mol Microbiol. 2004 Sep;53(6):1695-707
pubmed: 15341648
J Bacteriol. 1998 Oct;180(19):5030-7
pubmed: 9748433
Plant Cell. 2004 Dec;16(12):3496-507
pubmed: 15548740
Plant Physiol. 1999 Oct;121(2):345-51
pubmed: 10517825
Microbiology (Reading). 2004 Oct;150(Pt 10):3197-208
pubmed: 15470100
Plant Cell. 2012 Jan;24(1):322-35
pubmed: 22267486
Cell Microbiol. 2017 Mar;19(3):
pubmed: 27568483
Cell. 2006 May 19;125(4):749-60
pubmed: 16713565
Plant J. 2010 Oct;64(2):204-14
pubmed: 21070404
Appl Microbiol Biotechnol. 2011 Aug;91(4):1073-82
pubmed: 21626020
Plant Cell. 2014 Mar;26(3):1360-76
pubmed: 24642938
Mol Plant. 2014 May;7(5):874-92
pubmed: 24482436
Fungal Genet Biol. 2016 May;90:53-60
pubmed: 26688467
Plant Physiol. 1990 Dec;94(4):1849-54
pubmed: 16667926
Proc Natl Acad Sci U S A. 2007 Dec 4;104(49):19613-8
pubmed: 18042724
Plant Cell. 1993 Nov;5(11):1575-90
pubmed: 8312740
Plant Physiol. 1994 Apr;104(4):1113-1118
pubmed: 12232152
Nature. 2001 Sep 6;413(6851):36-7
pubmed: 11544516
Curr Genet. 2016 May;62(2):243-54
pubmed: 26527115
Plant Cell. 2011 Jun;23(6):2064-86
pubmed: 21653195
Mol Plant Microbe Interact. 2014 Feb;27(2):113-24
pubmed: 24200076
Cell Microbiol. 2016 Sep;18(9):1285-93
pubmed: 27306610
PLoS Pathog. 2012 Feb;8(2):e1002526
pubmed: 22346755
Heliyon. 2020 Jun 19;6(6):e04237
pubmed: 32596527
Cell Microbiol. 2010 Jul;12(7):863-72
pubmed: 20482553

Auteurs

Hang Liu (H)

Ministry of Agriculture and Rural Affairs Key Laboratory for Crop Pest Monitoring and Green Control, China Agricultural University, Beijing, 100193, China.

Xunli Lu (X)

Ministry of Agriculture and Rural Affairs Key Laboratory for Crop Pest Monitoring and Green Control, China Agricultural University, Beijing, 100193, China.

Mengfei Li (M)

Ministry of Agriculture and Rural Affairs Key Laboratory for Crop Pest Monitoring and Green Control, China Agricultural University, Beijing, 100193, China.

Zhiqin Lun (Z)

Ministry of Agriculture and Rural Affairs Key Laboratory for Crop Pest Monitoring and Green Control, China Agricultural University, Beijing, 100193, China.

Xia Yan (X)

The Sainsbury Laboratory, University of East Anglia, Norwich Research Park, Norwich, NR4 7UH, UK.

Changfa Yin (C)

Ministry of Agriculture and Rural Affairs Key Laboratory for Crop Pest Monitoring and Green Control, China Agricultural University, Beijing, 100193, China.

Guixin Yuan (G)

Ministry of Agriculture and Rural Affairs Key Laboratory for Crop Pest Monitoring and Green Control, China Agricultural University, Beijing, 100193, China.

Xingbin Wang (X)

Ministry of Agriculture and Rural Affairs Key Laboratory for Crop Pest Monitoring and Green Control, China Agricultural University, Beijing, 100193, China.

Ning Liu (N)

Ministry of Agriculture and Rural Affairs Key Laboratory for Crop Pest Monitoring and Green Control, China Agricultural University, Beijing, 100193, China.

Di Liu (D)

Ministry of Agriculture and Rural Affairs Key Laboratory for Crop Pest Monitoring and Green Control, China Agricultural University, Beijing, 100193, China.

Mian Wu (M)

Ministry of Agriculture and Rural Affairs Key Laboratory for Crop Pest Monitoring and Green Control, China Agricultural University, Beijing, 100193, China.

Ziluolong Luo (Z)

Ministry of Agriculture and Rural Affairs Key Laboratory for Crop Pest Monitoring and Green Control, China Agricultural University, Beijing, 100193, China.

Yan Zhang (Y)

Ministry of Agriculture and Rural Affairs Key Laboratory for Crop Pest Monitoring and Green Control, China Agricultural University, Beijing, 100193, China.

Vijai Bhadauria (V)

Ministry of Agriculture and Rural Affairs Key Laboratory for Crop Pest Monitoring and Green Control, China Agricultural University, Beijing, 100193, China.

Jun Yang (J)

Ministry of Agriculture and Rural Affairs Key Laboratory for Crop Pest Monitoring and Green Control, China Agricultural University, Beijing, 100193, China.

Nicholas J Talbot (NJ)

The Sainsbury Laboratory, University of East Anglia, Norwich Research Park, Norwich, NR4 7UH, UK.

You-Liang Peng (YL)

Ministry of Agriculture and Rural Affairs Key Laboratory for Crop Pest Monitoring and Green Control, China Agricultural University, Beijing, 100193, China. pengyl@cau.edu.cn.

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Classifications MeSH