Assessment of structural behaviour of a new L-asparaginase and SAXS data-based evidence for catalytic activity in its monomeric form.

Asparaginase / chemistry Molecular Docking Simulation Scattering, Small Angle X-Ray Diffraction Asparagine / chemistry
L-Asparaginase Molecular docking Monomer Pseudomonas resinovorans SAXS

Journal

International journal of biological macromolecules
ISSN: 1879-0003
Titre abrégé: Int J Biol Macromol
Pays: Netherlands
ID NLM: 7909578

Informations de publication

Date de publication:
31 Dec 2023
Historique:
received: 12 05 2023
revised: 30 08 2023
accepted: 06 09 2023
medline: 27 11 2023
pubmed: 10 9 2023
entrez: 9 9 2023
Statut: ppublish

Résumé

The present study reports the structural and functional characterization of a new glutaminase-free recombinant L-asparaginase (PrASNase) from Pseudomonas resinovorans IGS-131. PrASNase showed substrate specificity to L-asparagine, and its kinetic parameters, K

Identifiants

DOI: 10.1016/j.ijbiomac.2023.126803 PMID: 37689286
pubmed: 37689286
pii: S0141-8130(23)03700-5
doi: 10.1016/j.ijbiomac.2023.126803
pii:
doi:

Substances chimiques

Asparaginase EC 3.5.1.1
Asparagine 7006-34-0

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

126803

Informations de copyright

Copyright © 2023. Published by Elsevier B.V.

Déclaration de conflit d'intérêts

Declaration of competing interest The authors have declared that they have no conflict of interest related to this work.

Auteurs

Kanti N Mihooliya (KN)

Biochemical Engineering Research and Process Development Centre, CSIR-Institute of Microbial Technology, Chandigarh, India.

Jitender Nandal (J)

Biochemical Engineering Research and Process Development Centre, CSIR-Institute of Microbial Technology, Chandigarh, India.

Nidhi Kalidas (N)

GNR Advanced Protein Centre, CSIR-Institute of Microbial Technology, Chandigarh 160036, India.
GNR Advanced Protein Centre, CSIR-Institute of Microbial Technology, Chandigarh 160036, India.

Subhash Chand (S)

National Institute of Biologicals, Ministry of Health & Family Welfare, NOIDA, Uttar Pradesh, India.

Dipesh K Verma (DK)

Structural Biology Laboratory, CSIR-Institute of Microbial Technology, Chandigarh, India.

Mani S Bhattacharyya (MS)

Biochemical Engineering Research and Process Development Centre, CSIR-Institute of Microbial Technology, Chandigarh, India.

Debendra K Sahoo (DK)

Biochemical Engineering Research and Process Development Centre, CSIR-Institute of Microbial Technology, Chandigarh, India. Electronic address: debendra.sahoo@alumni.iitd.ac.in.

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Classifications MeSH

questionsmedicales.fr › Enzymes et coenzymes › Enzymes › Hydrolases › Amidohydrolases › Asparaginase › Assessment of structural behaviour of a new...
questionsmedicales.fr › Sciences de l'information › Méthodologies informatiques › Simulation numérique › Simulation de docking moléculaire › Assessment of structural behaviour of a new...
questionsmedicales.fr › Phénomènes physiques › Diffusion de rayonnements › Diffusion aux petits angles › Assessment of structural behaviour of a new...
questionsmedicales.fr › Phénomènes chimiques › Diffraction des rayons X › Assessment of structural behaviour of a new...
questionsmedicales.fr › Acides aminés, peptides et protéines › Acides aminés › Acides aminés neutres › Asparagine › Assessment of structural behaviour of a new...