Flexible Allyl-Modified Gelatin Photoclick Resin Tailored for Volumetric Bioprinting of Matrices for Soft Tissue Engineering.
adipose-derived stromal cells
allyl-modified gelatin
photoclick resins
soft hydrogels
volumetric bioprinting
Journal
Advanced healthcare materials
ISSN: 2192-2659
Titre abrégé: Adv Healthc Mater
Pays: Germany
ID NLM: 101581613
Informations de publication
Date de publication:
12 2023
12 2023
Historique:
revised:
11
08
2023
received:
27
03
2023
medline:
5
12
2023
pubmed:
12
9
2023
entrez:
12
9
2023
Statut:
ppublish
Résumé
Volumetric bioprinting (VBP) is a light-based 3D printing platform, which recently prompted a paradigm shift for additive manufacturing (AM) techniques considering its capability to enable the fabrication of complex cell-laden geometries in tens of seconds with high spatiotemporal control and pattern accuracy. A flexible allyl-modified gelatin (gelAGE)-based photoclick resin is developed in this study to fabricate matrices with exceptionally soft polymer networks (0.2-1.0 kPa). The gelAGE-based resin formulations are designed to exploit the fast thiol-ene crosslinking in combination with a four-arm thiolated polyethylene glycol (PEG4SH) in the presence of a photoinitiator. The flexibility of the gelAGE biomaterial platform allows one to tailor its concentration spanning from 2.75% to 6% and to vary the allyl to thiol ratio without hampering the photocrosslinking efficiency. The thiol-ene crosslinking enables the production of viable cell-material constructs with a high throughput in tens of seconds. The suitability of the gelAGE-based resins is demonstrated by adipogenic differentiation of adipose-derived stromal cells (ASC) after VBP and by the printing of more fragile adipocytes as a proof-of-concept. Taken together, this study introduces a soft photoclick resin which paves the way for volumetric printing applications toward soft tissue engineering.
Identifiants
pubmed: 37699146
doi: 10.1002/adhm.202300977
doi:
Substances chimiques
Gelatin
9000-70-8
Hydrogels
0
Sulfhydryl Compounds
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e2300977Subventions
Organisme : Deutsche Forschungsgemeinschaft
ID : 326998133
Organisme : Horizon 2020
ID : 874827
Informations de copyright
© 2023 The Authors. Advanced Healthcare Materials published by Wiley-VCH GmbH.
Références
a) J. Groll, T. Boland, T. Blunk, J. A. Burdick, D.-W. Cho, P. D. Dalton, B. Derby, G. Forgacs, Q. Li, V. A. Mironov, L. Moroni, M. Nakamura, W. Shu, S. Takeuchi, G. Vozzi, T. B. F. Woodfield, T. Xu, J. J. Yoo, J. Malda, Biofabrication 2016, 8, 013001;
b) L. Moroni, T. Boland, J. A. Burdick, C. De Maria, B. Derby, G. Forgacs, J. Groll, Q. Li, J. Malda, V. A. Mironov, C. Mota, M. Nakamura, W. Shu, S. Takeuchi, T. B. F. Woodfield, T. Xu, J. J. Yoo, G. Vozzi, Trends Biotechnol. 2018, 36, 384;
c) L. Moroni, J. A. Burdick, C. Highley, S. J. Lee, Y. Morimoto, S. Takeuchi, J. J. Yoo, Nat. Rev. Mater. 2018, 3, 21.
M. Shusteff, A. E. M. Browar, B. E. Kelly, J. Henriksson, T. H. Weisgraber, R. M. Panas, N. X. Fang, C. M. Spadaccini, Sci. Adv. 2017, 3, eaao5496.
B. E. Kelly, I. Bhattacharya, H. Heidari, M. Shusteff, C. M. Spadaccini, H. K. Taylor, Science 2019, 363, 1075.
P. N. Bernal, P. Delrot, D. Loterie, Y. Li, J. Malda, C. Moser, R. Levato, Adv. Mater. 2019, 31, 1904209.
a) K. Yue, G. Trujillo-De Santiago, M. M. Alvarez, A. Tamayol, N. Annabi, A. Khademhosseini, Biomaterials 2015, 73, 254;
b) B. J. Klotz, D. Gawlitta, A. J. W. P. Rosenberg, J. Malda, F. P. W. Melchels, Trends Biotechnol. 2016, 34, 394.
a) Z. Munoz, H. Shih, C.-C. Lin, Biomater. Sci. 2014, 2, 1063;
b) M. J. Kade, D. J. Burke, C. J. Hawker, J. Polym. Sci., Part A: Polym. Chem. 2010, 48, 743;
c) C. E. Hoyle, A. B. Lowe, C. N. Bowman, Chem. Soc. Rev. 2010, 39, 1355;
d) J. J. Roberts, S. J. Bryant, Biomaterials 2013, 34, 9969;
e) O. Llorente, A. Agirre, I. Calvo, M. Olaso, R. Tomovska, H. Sardon, Polym. J. 2021, 53, 1195.
R. Rizzo, D. Ruetsche, H. Liu, M. Zenobi-Wong, Adv. Mater. 2021, 33, 2102900.
S. Bertlein, G. Brown, K. S. Lim, T. Jungst, T. Boeck, T. Blunk, J. Tessmar, G. J. Hooper, T. B. F. Woodfield, J. Groll, Adv. Mater. 2017, 29, 1703404.
B. G. Soliman, G. C. J. Lindberg, T. Jungst, G. J. Hooper, J. Groll, T. B. F. Woodfield, K. S. Lim, Adv. Healthcare Mater. 2020, 9, e1901544.
D. Loterie, P. Delrot, C. Moser, Nat. Commun. 2020, 11, 852.
a) G. S. Major, J. W. Simcock, T. B. F. Woodfield, K. S. Lim, Trends Biotechnol. 2022, 40, 77;
b) C. S. Murphy, L. Liaw, M. R. Reagan, BMC Biomed. Eng. 2019, 1, 27.
a) I. Henriksson, P. Gatenholm, D. A. Hägg, Biofabrication 2017, 9, 015022;
b) F. B. Albrecht, V. Dolderer, S. Nellinger, F. F. Schmidt, P. J. Kluger, Gels 2022, 8, 420.
H. Horder, M. Guaza Lasheras, N. Grummel, A. Nadernezhad, J. Herbig, S. Ergün, J. Teßmar, J. Groll, B. Fabry, P. Bauer-Kreisel, T. Blunk, Cells 2021, 10, 803.
N. Contessi Negrini, N. Celikkin, P. Tarsini, S. Farè, W. Swieszkowski, Biofabrication 2020, 12, 025001.
J. Colle, P. Blondeel, A. De Bruyne, S. Bochar, L. Tytgat, C. Vercruysse, S. Van Vlierberghe, P. Dubruel, H. Declercq, J. Mater. Sci. Mater. Med. 2020, 31, 36.
L. Tytgat, L. Van Damme, M. D. P. O. Arevalo, H. Declercq, H. Thienpont, H. Otteveare, P. Blondeel, P. Dubruel, S. Van Vlierberghe, Int. J. Biol. Macromol. 2019, 140, 929.
J. K. Placone, A. J. Engler, Adv. Healthcare Mater. 2018, 7, 1701161.
H. Genç, A. Cianciosi, R. Lohse, P. Stahlhut, J. Groll, C. Alexiou, I. Cicha, T. Jüngst, Biomacromolecules 2023, 24, 1497.
X. Li, S. Chen, J. Li, X. Wang, J. Zhang, N. Kawazoe, G. Chen, Polymers 2016, 8, 269.
T. R. Cox, J. T. Erler, Dis. Model. Mech. 2011, 4, 165.
a) T. Morita, Y. Horikiri, T. Suzuki, H. Yoshino, Int. J. Pharm. 2001, 219, 127;
b) H. Jizomoto, J. Pharm. Sci. 1984, 73, 879;
c) H. Jizomoto, J. Pharm. Sci. 1985, 74, 469;
d) T. Nezu, H. Maeda, Bull. Chem. Soc. Jpn. 1991, 64, 1618.
M. Yanagisawa, Y. Yamashita, S.-A. Mukai, M. Annaka, M. Tokita, J. Mol. Liq. 2014, 200, 2.
M. W. Tibbitt, K. S. Anseth, Biotechnol. Bioeng. 2009, 103, 655.
K. S. Lim, B. J. Klotz, G. C. J. Lindberg, F. P. W. Melchels, G. J. Hooper, J. Malda, D. Gawlitta, T. B. F. Woodfield, Macromol. Biosci. 2019, 19, e1900098.
B. G. Soliman, A. Longoni, M. Wang, W. Li, P. N. Bernal, A. Cianciosi, G. C. J. Lindberg, J. Malda, J. Groll, T. Jungst, R. Levato, J. Rnjak-Kovacina, T. B. F. Woodfield, Y. S. Zhang, K. S. Lim, Adv. Funct. Mater. 2023, 33, 2210521.
L. Bian, C. Hou, E. Tous, R. Rai, R. L. Mauck, J. A. Burdick, Biomaterials 2013, 34, 413.
S. Lee, X. Tong, F. Yang, Acta Biomater. 2014, 10, 4167.
X. Unamuno, J. Gómez-Ambrosi, S. Becerril, F. J. Álvarez-Cienfuegos, B. Ramírez, A. Rodríguez, S. Ezquerro, V. Valentí, R. Moncada, A. Mentxaka, M. Llorente, C. Silva, M. D. L. R. Elizalde, V. Catalán, G. Frühbeck, Acta Biomater. 2022, 141, 264.
K. Elkhoury, M. Morsink, Y. Tahri, C. Kahn, F. Cleymand, S. R. Shin, E. Arab-Tehrany, L. Sanchez-Gonzalez, L. Sanchez-Gonzalez, Int. J. Biol. Macromol. 2021, 183, 918.
R. Levato, W. R. Webb, I. A. Otto, A. Mensinga, Y. Zhang, M. Van Rijen, R. Van Weeren, I. M. Khan, J. Malda, Acta Biomater. 2017, 61, 41.
K. Comley, N. Fleck, Int. J. Impact Eng. 2012, 46, 1.
K. Comley, N. A. Fleck, Int. J. Solids Struct. 2010, 47, 2982.
P. N. Bernal, M. Bouwmeester, J. Madrid-Wolff, M. Falandt, S. Florczak, N. G. Rodriguez, Y. Li, G. Groessbacher, R.-A. Samsom, M. Van Wolferen, L. J. W. Van Der Laan, P. Delrot, D. Loterie, J. Malda, C. Moser, B. Spee, R. Levato, Adv. Mater. 2022, 34, 2110054.
M. Xie, L. Lian, X. Mu, Z. Luo, C. E. Garciamendez-Mijares, Z. Zhang, A. López, J. Manríquez, X. Kuang, J. Wu, J. K. Sahoo, F. Z. González, G. Li, G. Tang, S. Maharjan, J. Guo, D. L. Kaplan, Y. S. Zhang, Nat. Commun. 2023, 14, 210.
W. Borchard, B. Burg, in Proc. 1990 Progress in Colloid & Polymer Science, Steinkopff, Heidelberg 2007, p. 83.
A. Matsumoto, J. Chen, A. L. Collette, U.-J. Kim, G. H. Altman, P. Cebe, D. L. Kaplan, J. Phys. Chem. B 2006, 110, 21630.
A. I. Van Den Bulcke, B. Bogdanov, N. De Rooze, E. H. Schacht, M. Cornelissen, H. Berghmans, Biomacromolecules 2000, 1, 31.
a) C. Michon, G. Cuvelier, B. Launay, Rheol. Acta 1993, 32, 94;
b) S.-H. Hsu, A. M. Jamieson, Polymer 1993, 34, 2602.
a) J. Malda, J. Visser, F. P. Melchels, T. Jüngst, W. E. Hennink, W. J. A. Dhert, J. Groll, D. W. Hutmacher, Adv. Mater. 2013, 25, 5011;
b) R. Levato, T. Jungst, R. G. Scheuring, T. Blunk, J. Groll, J. Malda, Adv. Mater. 2020, 32, 1906423.
W. L. Ng, J. M. Lee, M. Zhou, Y.-W. Chen, K.-X. A. Lee, W. Y. Yeong, Y.-F. Shen, Biofabrication 2020, 12, 022001.
Y. Chen, Y. Liu, J. Zhang, H. Liu, J. Wang, Q. Liu, Y. Zhang, Mater. Des. 2021, 200, 109467.
F. Louis, M. Piantino, H. Liu, D.-H. Kang, Y. Sowa, S. Kitano, M. Matsusaki, Cyborg Bionic Syst. 2021, 2021, 1412542.
a) L. Hyväri, M. Ojansivu, M. Juntunen, K. Kartasalo, S. Miettinen, S. Vanhatupa, Stem Cells Int. 2018, 2018, 2190657;
b) J.-J. Li, D. Xie, Biochem. Biophys. Res. Commun. 2007, 357, 648.
a) N. J. Hogrebe, J. W. Reinhardt, N. K. Tram, A. C. Debski, G. Agarwal, M. A. Reilly, K. J. Gooch, Acta Biomater. 2018, 70, 110;
b) J.-H. Hwang, M. R. Byun, A. R. Kim, K. M. Kim, H. J. Cho, Y. H. Lee, J. Kim, M. G. Jeong, E. S. Hwang, J.-H. Hong, PLoS One 2015, 10, e0135519;
c) D. A. Young, Y. S. Choi, A. J. Engler, K. L. Christman, Biomaterials 2013, 34, 8581.
D. Ribezzi, M. Gueye, S. Florczak, F. Dusi, D. De Vos, F. Manente, A. Hierholzer, M. Fussenegger, M. Caiazzo, T. Blunk, J. Malda, R. Levato, Adv. Mater. 2023, 35, 2301673.
a) Y. Li, A. S. Mao, B. R. Seo, X. Zhao, S. K. Gupta, M. Chen, Y. L. Han, T.-Y. Shih, D. J. Mooney, M. Guo, Sci. Adv. 2020, 6, eaax5611;
b) V. Pellegrinelli, J. Heuvingh, O. Du Roure, C. Rouault, A. Devulder, C. Klein, M. Lacasa, E. Clément, D. Lacasa, K. Clément, J. Pathol. 2014, 233, 183.
F. B. Albrecht, F. F. Schmidt, A.-C. Volz, P. J. Kluger, Gels 2022, 8, 611.
D. Contador, F. Ezquer, M. Espinosa, M. Arango-Rodriguez, C. Puebla, L. Sobrevia, P. Conget, Exp. Biol. Med. 2015, 240, 1235.
a) K. S. Lim, B. S. Schon, N. V. Mekhileri, G. C. J. Brown, C. M. Chia, S. Prabakar, G. J. Hooper, T. B. F. Woodfield, ACS Biomater. Sci. Eng. 2016, 2, 1752;
b) B. G. Soliman, G. S. Major, P. Atienza-Roca, C. A. Murphy, A. Longoni, C. R. Alcala-Orozco, J. Rnjak-Kovacina, D. Gawlitta, T. B. F. Woodfield, K. S. Lim, Adv. Healthcare Mater. 2022, 11, 2101873.
K. J. Livak, T. D. Schmittgen, Methods 2001, 25, 402.