CRISPR-based engineering of RNA viruses.


Journal

Science advances
ISSN: 2375-2548
Titre abrégé: Sci Adv
Pays: United States
ID NLM: 101653440

Informations de publication

Date de publication:
15 09 2023
Historique:
medline: 15 9 2023
pubmed: 13 9 2023
entrez: 13 9 2023
Statut: ppublish

Résumé

CRISPR RNA-guided endonucleases have enabled precise editing of DNA. However, options for editing RNA remain limited. Here, we combine sequence-specific RNA cleavage by CRISPR ribonucleases with programmable RNA repair to make precise deletions and insertions in RNA. This work establishes a recombinant RNA technology with immediate applications for the facile engineering of RNA viruses.

Identifiants

pubmed: 37703376
doi: 10.1126/sciadv.adj8277
pmc: PMC10499312
doi:

Substances chimiques

Endonucleases EC 3.1.-
RNA 63231-63-0

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

eadj8277

Subventions

Organisme : NIAID NIH HHS
ID : K99 AI171893
Pays : United States
Organisme : NIGMS NIH HHS
ID : R35 GM134867
Pays : United States
Organisme : NCATS NIH HHS
ID : TL1 TR002318
Pays : United States

Commentaires et corrections

Type : UpdateOf

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Auteurs

Artem Nemudryi (A)

Department of Microbiology and Cell Biology, Montana State University, Bozeman, MT 59717, USA.

Anna Nemudraia (A)

Department of Microbiology and Cell Biology, Montana State University, Bozeman, MT 59717, USA.

Joseph E Nichols (JE)

Department of Microbiology and Cell Biology, Montana State University, Bozeman, MT 59717, USA.

Andrew M Scherffius (AM)

Department of Microbiology and Cell Biology, Montana State University, Bozeman, MT 59717, USA.

Trevor Zahl (T)

Department of Microbiology and Cell Biology, Montana State University, Bozeman, MT 59717, USA.

Blake Wiedenheft (B)

Department of Microbiology and Cell Biology, Montana State University, Bozeman, MT 59717, USA.

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