Chromosome constitution and genetic relationships of Morus spp. revealed by genomic in situ hybridization.
Chromosome constitution
Genetic relationships
Genomic in situ hybridization
Morus
Mulberry section
Journal
BMC plant biology
ISSN: 1471-2229
Titre abrégé: BMC Plant Biol
Pays: England
ID NLM: 100967807
Informations de publication
Date de publication:
15 Sep 2023
15 Sep 2023
Historique:
received:
11
04
2023
accepted:
07
09
2023
medline:
18
9
2023
pubmed:
15
9
2023
entrez:
14
9
2023
Statut:
epublish
Résumé
Mulberry (Morus spp.) is an economically important woody plant, which has been used for sericulture (silk farming) for thousands of years. The genetic background of mulberry is complex due to polyploidy and frequent hybridization events. Comparative genomic in situ hybridization (cGISH) and self-GISH were performed to illustrate the chromosome constitution and genetic relationships of 40 mulberry accessions belonging to 12 species and three varietas in the Morus genus and containing eight different ploidy levels. We identified six homozygous cGISH signal patterns and one heterozygous cGISH signal pattern using four genomic DNA probes. Using cGISH and self-GISH data, we defined five mulberry sections (Notabilis, Nigra, Wittiorum, and Cathayana, all contained only one species; and Alba, which contained seven closely related species and three varietas, was further divided into two subsections) and proposed the genetic relationships among them. Differential cGISH signal patterns detected in section Alba allowed us to refine the genetic relationships among the closely related members of this section. We propose that GISH is an efficient tool to investigate the chromosome constitution and genetic relationships in mulberry. The results obtained here can be used to guide outbreeding of heterozygous perennial crops like mulberry.
Sections du résumé
BACKGROUND
BACKGROUND
Mulberry (Morus spp.) is an economically important woody plant, which has been used for sericulture (silk farming) for thousands of years. The genetic background of mulberry is complex due to polyploidy and frequent hybridization events.
RESULTS
RESULTS
Comparative genomic in situ hybridization (cGISH) and self-GISH were performed to illustrate the chromosome constitution and genetic relationships of 40 mulberry accessions belonging to 12 species and three varietas in the Morus genus and containing eight different ploidy levels. We identified six homozygous cGISH signal patterns and one heterozygous cGISH signal pattern using four genomic DNA probes. Using cGISH and self-GISH data, we defined five mulberry sections (Notabilis, Nigra, Wittiorum, and Cathayana, all contained only one species; and Alba, which contained seven closely related species and three varietas, was further divided into two subsections) and proposed the genetic relationships among them. Differential cGISH signal patterns detected in section Alba allowed us to refine the genetic relationships among the closely related members of this section.
CONCLUSIONS
CONCLUSIONS
We propose that GISH is an efficient tool to investigate the chromosome constitution and genetic relationships in mulberry. The results obtained here can be used to guide outbreeding of heterozygous perennial crops like mulberry.
Identifiants
pubmed: 37710184
doi: 10.1186/s12870-023-04448-9
pii: 10.1186/s12870-023-04448-9
pmc: PMC10503058
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
428Subventions
Organisme : Supported by State Key Laboratory of Silkworm Genome Biology
ID : sklsgb161718-9
Organisme : Chongqing Postdoctoral Science Foundation
ID : cstc2021jcyj-bshX0196
Organisme : Chongqing Postdoctoral Science Special Foundation
ID : 2010010005241167
Organisme : Supported by Fundamental Research Funds for the Central Universities
ID : SWU120028
Organisme : National Key Research and Development Program of China
ID : 2022YFD1201602
Organisme : Science and Technology Pilot Project - Key Research and Development Project
ID : SWU-XDZD22008
Informations de copyright
© 2023. BioMed Central Ltd., part of Springer Nature.
Références
PeerJ. 2019 Dec 12;7:e8158
pubmed: 31844573
PLoS One. 2015 Aug 12;10(8):e0135411
pubmed: 26266951
Ann Bot. 2011 Jul;108(1):113-21
pubmed: 21576078
Protoplasma. 2022 Jul;259(4):999-1011
pubmed: 34709474
Front Plant Sci. 2022 Jun 06;13:906168
pubmed: 35734244
Genome. 2013 Nov;56(11):641-9
pubmed: 24299103
PLoS One. 2016 Apr 05;11(4):e0153080
pubmed: 27046026
Proc Natl Acad Sci U S A. 2004 Sep 14;101(37):13554-9
pubmed: 15342909
Hortic Res. 2022 Jan 5;9:
pubmed: 35043186
Genomics Proteomics Bioinformatics. 2022 Dec;20(6):1119-1137
pubmed: 36055564
Mol Ecol. 2021 Nov;30(22):5780-5795
pubmed: 34487579
Genome. 2006 Sep;49(9):1057-68
pubmed: 17110986
J Agric Food Chem. 2020 Feb 5;68(5):1494-1504
pubmed: 31917553
Gene. 2020 Feb 5;726:144162
pubmed: 31639429
Sci Rep. 2017 Aug 29;7(1):9573
pubmed: 28852033
J Genet Genomics. 2007 May;34(5):437-48
pubmed: 17560530
BMC Plant Biol. 2001;1:3
pubmed: 11801190
Genet Mol Res. 2013 Aug 12;12(3):2953-65
pubmed: 24065651
Mol Cytogenet. 2018 Jun 07;11:35
pubmed: 29977338
Theor Appl Genet. 2021 Oct;134(10):3209-3224
pubmed: 34160642
Methods Mol Biol. 2014;1115:325-36
pubmed: 24415482
Genomics. 2021 May;113(3):1180-1192
pubmed: 33677055
Nat Commun. 2013;4:2445
pubmed: 24048436
Mol Plant. 2020 Jul 6;13(7):1001-1012
pubmed: 32422187
Genome. 2004 Jun;47(3):439-48
pubmed: 15190361
Plants (Basel). 2022 May 24;11(11):
pubmed: 35684163
Methods Mol Biol. 2016;1429:151-60
pubmed: 27511173
Mol Biol Rep. 2019 Apr;46(2):1809-1817
pubmed: 30694457