Identification of enzymatic functions of osmo-regulated periplasmic glucan biosynthesis proteins from Escherichia coli reveals a novel glycoside hydrolase family.
Journal
Communications biology
ISSN: 2399-3642
Titre abrégé: Commun Biol
Pays: England
ID NLM: 101719179
Informations de publication
Date de publication:
21 09 2023
21 09 2023
Historique:
received:
04
05
2023
accepted:
08
09
2023
medline:
25
9
2023
pubmed:
22
9
2023
entrez:
22
9
2023
Statut:
epublish
Résumé
Most Gram-negative bacteria synthesize osmo-regulated periplasmic glucans (OPG) in the periplasm or extracellular space. Pathogenicity of many pathogens is lost by knocking out opgG, an OPG-related gene indispensable for OPG synthesis. However, the biochemical functions of OpgG and OpgD, a paralog of OpgG, have not been elucidated. In this study, structural and functional analyses of OpgG and OpgD from Escherichia coli revealed that these proteins are β-1,2-glucanases with remarkably different activity from each other, establishing a new glycoside hydrolase family, GH186. Furthermore, a reaction mechanism with an unprecedentedly long proton transfer pathway among glycoside hydrolase families is proposed for OpgD. The conformation of the region that forms the reaction pathway differs noticeably between OpgG and OpgD, which explains the observed low activity of OpgG. The findings enhance our understanding of OPG biosynthesis and provide insights into functional diversity for this novel enzyme family.
Identifiants
pubmed: 37735577
doi: 10.1038/s42003-023-05336-6
pii: 10.1038/s42003-023-05336-6
pmc: PMC10514313
doi:
Substances chimiques
Glycoside Hydrolases
EC 3.2.1.-
Glucans
0
Periplasmic Proteins
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
961Informations de copyright
© 2023. Springer Nature Limited.
Références
Carbohydr Res. 1995 Dec 20;278(2):205-25
pubmed: 8590443
J Mol Biol. 2004 Sep 3;342(1):195-205
pubmed: 15313617
J Bacteriol. 1999 Jun;181(12):3626-31
pubmed: 10368134
BMC Biotechnol. 2015 Jun 03;15:47
pubmed: 26037246
J Mol Biol. 2007 Sep 21;372(3):774-97
pubmed: 17681537
Acta Crystallogr D Biol Crystallogr. 2004 Dec;60(Pt 12 Pt 1):2126-32
pubmed: 15572765
J Bacteriol. 2004 Jun;186(12):3695-702
pubmed: 15175282
J Biol Chem. 1984 Jul 10;259(13):8388-93
pubmed: 6330111
Protein Sci. 2020 Jan;29(1):128-140
pubmed: 31606894
Acta Crystallogr D Biol Crystallogr. 2010 Jan;66(Pt 1):22-5
pubmed: 20057045
PLoS One. 2016 Feb 17;11(2):e0148870
pubmed: 26886583
Nucleic Acids Res. 2016 Jul 8;44(W1):W344-50
pubmed: 27166375
Microbiology (Reading). 2008 Feb;154(Pt 2):476-483
pubmed: 18227251
Proc Natl Acad Sci U S A. 1986 Oct;83(19):7236-40
pubmed: 2945202
J Biol Chem. 2015 Oct 16;290(42):25657-69
pubmed: 26304114
Eur J Biochem. 2002 May;269(10):2473-84
pubmed: 12027885
FEBS Lett. 2017 Dec;591(23):3926-3936
pubmed: 29131329
J Biol Chem. 2019 May 10;294(19):7942-7965
pubmed: 30926603
FEMS Microbiol Lett. 1993 Dec 1;114(2):139-44
pubmed: 8282182
J Biol Chem. 1983 Feb 25;258(4):2394-8
pubmed: 6296144
Acta Crystallogr D Biol Crystallogr. 1997 May 1;53(Pt 3):240-55
pubmed: 15299926
Proc Natl Acad Sci U S A. 1986 Jun;83(12):4403-7
pubmed: 16593714
J Bacteriol. 1996 Aug;178(15):4635-42
pubmed: 8755895
J Bacteriol. 1996 Apr;178(8):2263-71
pubmed: 8636027
J Comput Chem. 2009 Dec;30(16):2785-91
pubmed: 19399780
J Bacteriol. 2022 Apr 19;204(4):e0061221
pubmed: 35311558
Microbiology (Reading). 1997 Apr;143 ( Pt 4):1115-1124
pubmed: 9141674
Nucleic Acids Res. 2005 Jul 1;33(Web Server issue):W299-302
pubmed: 15980475
Microbiol Rev. 1994 Jun;58(2):145-61
pubmed: 8078434
FEBS J. 2013 Jan;280(1):184-98
pubmed: 23137336
J Chem Inf Model. 2013 Aug 26;53(8):1893-904
pubmed: 23379370
Nat Struct Mol Biol. 2022 Dec;29(12):1170-1177
pubmed: 36456825
FEBS J. 2012 Sep;279(17):3185-91
pubmed: 22776355
Biomed Res Int. 2013;2013:371429
pubmed: 24228245
J Bacteriol. 1984 Dec;160(3):976-81
pubmed: 6094515
Nat Biotechnol. 2019 Apr;37(4):420-423
pubmed: 30778233
Mol Microbiol. 1991 Jul;5(7):1745-53
pubmed: 1834913
Anal Biochem. 2021 Nov 1;632:114366
pubmed: 34509443
Acta Crystallogr D Biol Crystallogr. 2006 Sep;62(Pt 9):1002-11
pubmed: 16929101
J Bacteriol. 2021 May 20;203(12):e0051520
pubmed: 33846116
Nucleic Acids Res. 2022 Jan 7;50(D1):D571-D577
pubmed: 34850161
Acta Crystallogr D Biol Crystallogr. 2010 Feb;66(Pt 2):125-32
pubmed: 20124692
J Mol Biol. 2013 Nov 15;425(22):4468-78
pubmed: 23954514
Mol Biol Evol. 2021 Jun 25;38(7):3022-3027
pubmed: 33892491
J Am Chem Soc. 2002 Aug 28;124(34):10015-24
pubmed: 12188666
Environ Microbiol Rep. 2015 Oct;7(5):690-7
pubmed: 26265506
Nucleic Acids Res. 2023 Jan 6;51(D1):D418-D427
pubmed: 36350672
Can J Microbiol. 2002 Nov;48(11):1008-16
pubmed: 12556128
Eur J Biochem. 2002 May;269(10):2464-72
pubmed: 12027884
Cell Chem Biol. 2017 Apr 20;24(4):515-524.e5
pubmed: 28392148
Nucleic Acids Res. 2003 Jul 1;31(13):3320-3
pubmed: 12824317
Acta Biochim Pol. 2003;50(4):1273-81
pubmed: 14740013
PLoS One. 2014 Mar 19;9(3):e92353
pubmed: 24647662
Anal Biochem. 2018 Nov 1;560:1-6
pubmed: 30149026
J Biol Chem. 2017 May 5;292(18):7487-7506
pubmed: 28270506
Glycobiology. 2016 Nov;26(11):1157-1170
pubmed: 27550196
J Chem Theory Comput. 2011 Feb 8;7(2):525-37
pubmed: 26596171
FEBS Lett. 2005 Jan 17;579(2):302-12
pubmed: 15642336
EcoSal Plus. 2017 Jun;7(2):
pubmed: 28593831
Protein Sci. 1995 Nov;4(11):2411-23
pubmed: 8563639
Nucleic Acids Res. 2008 Apr;36(7):2295-300
pubmed: 18287115